Date published: 2025-11-20

1-800-457-3801

SCBT Portrait Logo
Seach Input

20S Proteasome α5 Antibody (D-9): sc-271378

4.3(3)
Write a reviewAsk a question

Datasheets
  • 20S Proteasome α5 Antibody (D-9) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 41-93 mapping near the N-terminus of 20S Proteasome α5 of human origin
  • recommended for detection of 20S Proteasome α5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 20S Proteasome α5 Antibody (D-9) for WB applications. These reagents are now offered in bundles with 20S Proteasome α5 Antibody (D-9) (see ordering information below).

QUICK LINKS

SEE ALSO...

20S Proteasome α5 Antibody (D-9) is a mouse monoclonal IgG1 antibody that detects PSMA5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PSMA5 is a crucial component of the 26S proteasome, which plays a vital role in maintaining cellular homeostasis by degrading unnecessary or damaged proteins through proteolysis. This process is essential for regulating various cellular functions, including cell cycle, signal transduction, and response to oxidative stress. The 20S core particle, where actual degradation occurs, is composed of multiple subunits that form a cylindrical structure, allowing for selective entry of target proteins while preventing unregulated access. PSMA5 contributes to structural integrity of the proteasome and is involved in assembly of the complex, ensuring efficient protein turnover. Anti-PSMA5 antibody (D-9) enables researchers to gain insights into proteasome activity and implications in various diseases, including cancer and neurodegenerative disorders, where proteasome function is often compromised.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

20S Proteasome α5 Antibody (D-9)

sc-271378
200 µg/ml
$316.00

20S Proteasome α5 Antibody (D-9): m-IgG Fc BP-HRP Bundle

sc-537625
200 µg Ab; 10 µg BP
$354.00

20S Proteasome α5 Antibody (D-9): m-IgGκ BP-HRP Bundle

sc-534944
200 µg Ab; 40 µg BP
$354.00

20S Proteasome α5 Antibody (D-9): m-IgG1 BP-HRP Bundle

sc-545265
200 µg Ab; 20 µg BP
$354.00

What is the recommended fixation method for immunofluorescence staining with 20S Proteasome α5 (D-9): sc-271378 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_11, d_19, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_271378, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 115ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic stainingSuperb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-09
Rated 4 out of 5 by from Strong band seen in WB using HeLa whole cellStrong band seen in WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-09
  • y_2025, m_11, d_19, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_271378, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 41ms
  • REVIEWS, PRODUCT
20S Proteasome α5 Antibody (D-9) is rated 4.3 out of 5 by 3.
  • y_2025, m_11, d_19, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_271378, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 128ms
  • REVIEWS, PRODUCT