20S Proteasome α5 Antibody (B-9): sc-137240

20S Proteasome α5 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects 20S Proteasome α5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-20S Proteasome α5 antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 20S proteasome plays a crucial role in cellular protein homeostasis by degrading unnecessary or damaged proteins, a process vital for maintaining cellular function and regulating various biological pathways. Located in the cytoplasm and nucleus, the 20S proteasome is a core component of the ubiquitin-proteasome system, which tags proteins for degradation through ubiquitination. This system is essential for controlling the concentration of specific proteins, thus influencing processes such as cell cycle progression, apoptosis, and responses to oxidative stress. The structural integrity of the 20S proteasome, composed of a hollow core formed by 28 subunits arranged in a cylindrical shape, allows for the efficient entry and degradation of substrates, underscoring its importance in cellular regulation and signaling.

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20S Proteasome α5 Antibody (B-9) References:

1. Primary structural features of the 20S proteasome subunits of rice (Oryza sativa).  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
2. Induction of protein catabolism and the ubiquitin-proteasome pathway by mild oxidative stress.  |  Gomes-Marcondes, MC. and Tisdale, MJ. 2002. Cancer Lett. 180: 69-74. PMID: 11911972
3. Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal.  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
4. Comprehensive mass spectrometric analysis of the 20S proteasome complex.  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
5. Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors.  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
6. Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus.  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253
7. cDNA cloning, characterization, and developmental expression of the 20S proteasome alpha5 subunit in the Mediterranean fruit fly Ceratitis capitata.  |  Verras, M., et al. 2008. Arch Insect Biochem Physiol. 67: 120-9. PMID: 18163525
8. 20S proteasome activity is modified via S-glutathionylation based on intracellular redox status of the yeast Saccharomyces cerevisiae: implications for the degradation of oxidized proteins.  |  Demasi, M., et al. 2014. Arch Biochem Biophys. 557: 65-71. PMID: 24813691
9. Ordered structure of the crystallized bovine 20S proteasome.  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
10. Conformational constraints in protein degradation by the 20S proteasome.  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
11. Human proteasome subunits from 2-dimensional gels identified by partial sequencing.  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
12. Structure and structure formation of the 20S proteasome.  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-20S Proteasome α5 Antibody (B-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-20S Proteasome α5 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

20S Proteasome α5 Antibody (B-9) Specifications

Alternate Product Name: 20S Proteasome α5

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 195-232

Epitope Target: 20S Proteasome α5

Epitope Species: human

Product Reactivity/Detection: 20S Proteasome α5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for 20S Proteasome α5 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin