17β-HSD7 Antibody (F-4): sc-393936
- 17β-HSD7 Antibody (F-4) is a mouse monoclonal IgG2a κ 17β-HSD7 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 121-195 mapping within an internal region of 17β-HSD7 of human origin
- 17 beta-HSD7 Antibody (F-4) is recommended for detection of 17β-HSD7 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-17 beta-HSD7 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for 17β-HSD7 Antibody (F-4) for WB applications. These reagents are now offered in bundles with 17β-HSD7 Antibody (F-4) (see ordering information below).
17β-HSD7 Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects 17β-HSD7 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). 17β-HSD7 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 17β-HSD7, also known as 3-keto-steroid reductase, is a crucial enzyme in the 17β-hydroxysteroid dehydrogenase family, which plays a significant role in regulating steroid availability across different tissues. This 341 amino acid protein is primarily responsible for converting estrone to estradiol, a vital process for maintaining hormonal balance and supporting reproductive health. 17β-HSD7 is involved in cholesterol biosynthesis, highlighting its importance in lipid metabolism. 17β-HSD7 is predominantly expressed in the adrenal gland, liver, lung, and thymus, and plays a critical role in the corpus luteum, where 17β-HSD7 is believed to contribute to fetal development. Single nucleotide polymorphisms in the gene encoding 17β-HSD7 can influence transcription levels in LNCaP and DU145 cells, potentially modulating adverse reactions induced by estramustine phosphate sodium, making 17β-HSD7 a significant target for research in steroid metabolism and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
17β-HSD7 Antibody (F-4) References:
- Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis. | Marijanovic, Z., et al. 2003. Mol Endocrinol. 17: 1715-25. PMID: 12829805
- Cloning and characterization of a 5' regulatory region of the prolactin receptor-associated protein/17{beta} hydroxysteroid dehydrogenase 7 gene. | Risk, M., et al. 2005. Endocrinology. 146: 2807-16. PMID: 15731358
- 17Beta-hydroxysteroid dehydrogenase type 7 (Hsd17b7) reverts cholesterol auxotrophy in NS0 cells. | Seth, G., et al. 2006. J Biotechnol. 121: 241-52. PMID: 16126295
- Analysis of the 5' flanking regions of human and murine HSD17B7: identification of a cholesterol dependent enhancer region. | Ohnesorg, T. and Adamski, J. 2006. Mol Cell Endocrinol. 248: 164-7. PMID: 16356630
- Transcriptional regulation of human and murine 17beta-hydroxysteroid dehydrogenase type-7 confers its participation in cholesterol biosynthesis. | Ohnesorg, T., et al. 2006. J Mol Endocrinol. 37: 185-97. PMID: 16901934
- Prolactin receptor-associated protein/17beta-hydroxysteroid dehydrogenase type 7 gene (Hsd17b7) plays a crucial role in embryonic development and fetal survival. | Shehu, A., et al. 2008. Mol Endocrinol. 22: 2268-77. PMID: 18669642
- Single nucleotide polymorphisms of 17beta-hydroxysteroid dehydrogenase type 7 gene: mechanism of estramustine-related adverse reactions? | Ozeki, T., et al. 2009. Int J Urol. 16: 836-41. PMID: 19735314
- Expression cloning of a novel estrogenic mouse 17 beta-hydroxysteroid dehydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRAP) in rat. | Nokelainen, P., et al. 1998. Mol Endocrinol. 12: 1048-59. PMID: 9658408
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
17β-HSD7 Antibody (F-4) | sc-393936 | 200 µg/ml | $316.00 | |||
17β-HSD7 Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-526278 | 200 µg Ab; 10 µg BP | $354.00 | |||
17β-HSD7 Antibody (F-4): m-IgG2a BP-HRP Bundle | sc-546558 | 200 µg Ab; 10 µg BP | $354.00 | |||
17β-HSD7 Antibody (F-4) AC | sc-393936 AC | 500 µg/ml, 25% agarose | $416.00 | |||
17β-HSD7 Antibody (F-4) HRP | sc-393936 HRP | 200 µg/ml | $316.00 | |||
17β-HSD7 Antibody (F-4) FITC | sc-393936 FITC | 200 µg/ml | $330.00 | |||
17β-HSD7 Antibody (F-4) PE | sc-393936 PE | 200 µg/ml | $343.00 | |||
17β-HSD7 Antibody (F-4) Alexa Fluor® 488 | sc-393936 AF488 | 200 µg/ml | $357.00 | |||
17β-HSD7 Antibody (F-4) Alexa Fluor® 546 | sc-393936 AF546 | 200 µg/ml | $357.00 | |||
17β-HSD7 Antibody (F-4) Alexa Fluor® 594 | sc-393936 AF594 | 200 µg/ml | $357.00 | |||
17β-HSD7 Antibody (F-4) Alexa Fluor® 647 | sc-393936 AF647 | 200 µg/ml | $357.00 | |||
17β-HSD7 Antibody (F-4) Alexa Fluor® 680 | sc-393936 AF680 | 200 µg/ml | $357.00 | |||
17β-HSD7 Antibody (F-4) Alexa Fluor® 790 | sc-393936 AF790 | 200 µg/ml | $357.00 |