Date published: 2026-4-5
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14-3-3 θ Antibody (3B9) - Western Blotting - Image 67300
14-3-3 q (3B9): sc-59414. Western blot analysis of 14-3-3 q expression in NIH/3T3 (A), PC-12 (B), Madin-Darby bovine kidney (C) and human fibroblast (D) whole cell lysates.
14-3-3 q (3B9): sc-59414. Western blot analysis of 14-3-3 q expression in NIH/3T3 (A), PC-12 (B), Madin-Darby bovine kidney (C) and human fibroblast (D) whole cell lysates.

14-3-3 θ Antibody (3B9): sc-59414

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Datasheets
  • 14-3-3 θ Antibody (3B9) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
  • raised against full length 14-3-3 θ of human origin
  • recommended for detection of 14-3-3 θ of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including bovine; non cross-reactive with 14-3-3 ζ
  • See pan 14-3-3 (B-8): sc-133233 for 14-3-3 θ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgGκ BP-HRP is the preferred secondary detection reagent for 14-3-3 θ Antibody (3B9) for WB and IHC(P) applications. This reagent is now offered in a bundle with 14-3-3 θ Antibody (3B9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Additional 14-3-3 Antibodies including 14-3-3 β, 14-3-3 ε, 14-3-3 η, 14-3-3 γ, 14-3-3 σ, 14-3-3 ζ and pan 14-3-3

14-3-3 θ Antibody (3B9) is a mouse monoclonal IgG1 antibody that detects 14-3-3 θ in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). 14-3-3 θ is a member of the 14-3-3 protein family, which plays a crucial role in regulating numerous cellular processes, particularly in cancer biology. 14-3-3 θ is primarily located in the cytoplasm, where 14-3-3 θ acts as a key regulator of signal transduction pathways by binding to phosphorylated serine and threonine residues on target proteins. The cytoplasmic localization of 14-3-3 θ enables modulation of various signaling cascades, including those involved in cell growth, survival, and apoptosis. Through sequestering target proteins in the cytoplasm, 14-3-3 θ prevents protein degradation and influences protein activity, thereby impacting critical cellular functions. Dysregulation of 14-3-3 θ expression or function can lead to aberrant signaling and contribute to the development of various diseases, including cancer, making 14-3-3 θ an important target for research and therapeutic interventions.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

14-3-3 θ Antibody (3B9) References:

  1. Climbing fiber activity reduces 14-3-3-θ regulated GABA(A) receptor phosphorylation in cerebellar Purkinje cells.  |  Qian, Z., et al. 2012. Neuroscience. 201: 34-45. PMID: 22119642
  2. α-Synuclein overexpression represses 14-3-3θ transcription.  |  Ding, H., et al. 2013. J Mol Neurosci. 51: 1000-9. PMID: 23912650
  3. 14-3-3θ facilitates plasma membrane delivery and function of mechanosensitive connexin 43 hemichannels.  |  Batra, N., et al. 2014. J Cell Sci. 127: 137-46. PMID: 24163432
  4. 14-3-3 inhibition promotes dopaminergic neuron loss and 14-3-3θ overexpression promotes recovery in the MPTP mouse model of Parkinson's disease.  |  Ding, H., et al. 2015. Neuroscience. 307: 73-82. PMID: 26314634
  5. Regulation of the Water Channel Aquaporin-2 via 14-3-3θ and -ζ.  |  Moeller, HB., et al. 2016. J Biol Chem. 291: 2469-84. PMID: 26645691
  6. Crosstalk between 14-3-3θ and AF4 enhances MLL-AF4 activity and promotes leukemia cell proliferation.  |  Fioretti, T., et al. 2019. Cell Oncol (Dordr). 42: 829-845. PMID: 31493143
  7. Schisandrol A Attenuates Myocardial Ischemia/Reperfusion-Induced Myocardial Apoptosis through Upregulation of 14-3-3θ.  |  Gong, S., et al. 2021. Oxid Med Cell Longev. 2021: 5541753. PMID: 34257806
  8. 14-3-3θ Does Not Protect against Behavioral or Pathological Deficits in Alzheimer's Disease Mouse Models.  |  Gannon, M., et al. 2022. eNeuro. 9: PMID: 35697511
  9. 14-3-3θ, a novel player in TDP-43 pathophysiology: Implications for ALS/FTD.  |  Khalil, B. and Da Cruz, S. 2024. Neuron. 112: 1197-1199. PMID: 38636451
  10. 14-3-3 phosphorylation inhibits 14-3-3θ's ability to regulate LRRK2 kinase activity and toxicity.  |  Pattanayak, R., et al. 2024. Hum Mol Genet. 33: 2071-2083. PMID: 39324210

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

14-3-3 θ Antibody (3B9)

sc-59414
200 µg/ml
$322.00

14-3-3 θ Antibody (3B9): m-IgGκ BP-HRP Bundle

sc-551945
200 µg Ab; 40 µg BP
$361.00

I would like to use 14-3-3 θ (3B9): sc-59414 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Asked by: Trav11
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Answered by: Technical Support
Date published: 2017-04-12
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Rated 5 out of 5 by from Very robust signal in WB in nonVery robust signal in WB in non-transfected 293T, human 14-3-3 transfected 293T and A-431 whole cell lysates and mouse placenta tissue extract. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Great specificity in WB in NIHGreat specificity in WB in NIH-3T3, PC-12, Madin-Darby bovine kidney and human fibroblast whole cell lysates. -SCBT QC
Date published: 2015-04-04
Rated 4 out of 5 by from Clear WB band in HeLa whole cell lysateClear WB band in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-15
Rated 4 out of 5 by from Strong signal for WB in nonStrong signal for WB in non-transfected 293T, human 14-3-3 transfected 293T and NIH/3T3 whole cell lysates. Possible post-translational modification. -SCBT QC
Date published: 2013-06-24
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14-3-3 θ Antibody (3B9) is rated 4.5 out of 5 by 4.
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