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14-3-3 γ Antibody (6A1) - Western Blotting - Image 22054
14-3-3 g (6A1): sc-69955. Western blot analysis of 14-3-3 g expression in non-transfected 293T: sc-117752 (A), human 14-3-3 g transfected 293T: sc-113231 (B) and K-562 (C) whole cell lysates.
14-3-3 γ Antibody (6A1) - Western Blotting - Image 22054
14-3-3 g (6A1): sc-69955. Western blot analysis of 14-3-3 g expression in non-transfected 293T: sc-117752 (A), human 14-3-3 g transfected 293T: sc-113231 (B) and K-562 (C) whole cell lysates.
14-3-3 g (6A1): sc-69955. Western blot analysis of 14-3-3 g expression in non-transfected 293T: sc-117752 (A), human 14-3-3 g transfected 293T: sc-113231 (B) and K-562 (C) whole cell lysates.

14-3-3 γ Antibody (6A1): sc-69955

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Datasheets
  • 14-3-3 γ Antibody (6A1) is a mouse monoclonal IgG2a, cited in 4 publications, provided at 50 µg/0.5 ml
  • raised against the N-terminus of 14-3-3 γ of human origin
  • recommended for detection of 14-3-3 γ where the N-terminal Met is removed, resulting in an acetylated N-terminal Valine of mouse, rat and human origin by WB and IP; not recommended for detection of unprocessed (non-modified) 14-3-3 γ
  • See pan 14-3-3 (B-8): sc-133233 for 14-3-3 γ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for 14-3-3 γ Antibody (6A1). This work is in progress.
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Additional 14-3-3 Antibodies including 14-3-3 β, 14-3-3 ε, 14-3-3 η, 14-3-3 γ, 14-3-3 σ, 14-3-3 θ, 14-3-3 ζ and pan 14-3-3

14-3-3 γ Antibody (6A1) is a mouse monoclonal IgG2a antibody that detects 14-3-3 γ in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). 14-3-3 γ is a member of the 14-3-3 protein family, which plays a crucial role in regulating various cellular processes, including apoptosis, mitogenic signaling, and cell-cycle checkpoints, all vital for maintaining cellular homeostasis and preventing cancer progression. 14-3-3 γ is primarily located in the cytoplasm, where 14-3-3 γ acts as a scaffolding protein that facilitates interaction between different signaling molecules. Cytoplasmic localization of 14-3-3 γ allows for rapid modulation of signaling pathways in response to cellular stimuli, ensuring appropriate cellular responses are activated or inhibited as needed. By binding to phosphorylated serine and threonine residues on target proteins, 14-3-3 γ can influence stability, localization, and activity, thereby playing a pivotal role in regulating key cellular functions. Formation of dimers and interaction with various ligands by 14-3-3 γ underscores importance in cellular signaling networks, and any loss of expression or dysfunction can lead to a range of pathological conditions, including cancer.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

14-3-3 γ Antibody (6A1) References:

  1. Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics.  |  Lv, J., et al. 2008. Fertil Steril. 90: 1892-8. PMID: 18054924
  2. 14-3-3 Gamma is required to enforce both the incomplete S phase and G2 DNA damage checkpoints.  |  Hosing, AS., et al. 2008. Cell Cycle. 7: 3171-9. PMID: 18843201
  3. 14-3-3γ protein attenuates lipopolysaccharide-induced cardiomyocytes injury through the Bcl-2 family/mitochondria pathway.  |  Liu, D., et al. 2014. Int Immunopharmacol. 21: 509-15. PMID: 24957688
  4. 14-3-3γ regulates Copine1-mediated neuronal differentiation in HiB5 hippocampal progenitor cells.  |  Cheal Yoo, J., et al. 2017. Exp Cell Res. 356: 85-92. PMID: 28412242
  5. Direct interaction with 14-3-3γ promotes surface expression of Best1 channel in astrocyte.  |  Oh, SJ., et al. 2017. Mol Brain. 10: 51. PMID: 29121962
  6. Tetramethylpyrazine Attenuates the Endotheliotoxicity and the Mitochondrial Dysfunction by Doxorubicin via 14-3-3γ/Bcl-2.  |  Yang, B., et al. 2019. Oxid Med Cell Longev. 2019: 5820415. PMID: 31885804
  7. 14-3-3γ, a novel regulator of the large-conductance Ca2+-activated K+ channel.  |  Chen, S., et al. 2020. Am J Physiol Renal Physiol. 319: F52-F62. PMID: 32463725
  8. Puerarin attenuates lipopolysaccharide-induced myocardial injury via the 14-3-3γ/PKCε pathway activating adaptive autophagy.  |  Peng, Y., et al. 2022. Int Immunopharmacol. 108: 108905. PMID: 35729836
  9. 14-3-3γ mediates the long-term inhibition of peripheral kappa opioid receptor antinociceptive signaling by norbinaltorphimine.  |  Wedemeyer, MJ., et al. 2022. Neuropharmacology. 220: 109251. PMID: 36126728
  10. The 14-3-3γ isoform binds to and regulates the localization of endoplasmic reticulum (ER) membrane protein TMCC3 for the reticular network of the ER.  |  Suhda, S., et al. 2023. J Biol Chem. 299: 102813. PMID: 36549645

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

14-3-3 γ Antibody (6A1)

sc-69955
50 µg/0.5 ml
$322.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with 14-3-3 γ (6A1): sc-69955 antibody?

Asked by: jerojero
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from excellent antibody for westernI bought 14-3-3r 3 month ago. This antibody works really well in western. I only need to use 1:1500 dilution and give very strong signal.
Date published: 2016-12-12
Rated 4 out of 5 by from WB data shows strong signal in nonWB data shows strong signal in non-transfected 293T, human 14-3-3 transfected 293T and K-562 whole cell lysates. -SCBT QC
Date published: 2015-01-09
Rated 5 out of 5 by from Good data with breast cancer and noncancerousGood data with breast cancer and noncancerous tissues in IHC. -SCBT Publication Review
Date published: 2013-02-20
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14-3-3 γ Antibody (6A1) is rated 4.7 out of 5 by 3.
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