14-3-3 γ Antibody (D-6): sc-398423
- 14-3-3 γ Antibody (D-6) is a mouse monoclonal IgG1 κ 14-3-3 γ antibody, cited in 10 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 128-149 within an internal region of 14-3-3 γ of human origin
- 14-3-3 gamma Antibody (D-6) is recommended for detection of 14-3-3 γ of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-14-3-3 gamma Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for 14-3-3 γ Antibody (D-6) for WB applications. This reagent is now offered in a bundle with 14-3-3 γ Antibody (D-6) (see ordering information below).
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14-3-3 γ Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects 14-3-3 gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-14-3-3 gamma antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 14-3-3 gamma protein plays a crucial role in cellular signaling and regulation, particularly in cancer biology, where 14-3-3 gamma is involved in processes such as apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 gamma protein is primarily located in the cytoplasm, where 14-3-3 gamma interacts with various target proteins, facilitating proper function and localization. Cytoplasmic presence of 14-3-3 proteins is essential for sequestering and regulating ligands, which can prevent degradation and modulate enzymatic activity. The 14-3-3 family consists of seven isoforms, including 14-3-3 β, γ, ε, ζ, η, θ, and Σ, which form dimers that create binding sites for ligands sharing a common 14-3-3 consensus binding motif, often characterized by serine/threonine phosphorylation. Dysregulation or loss of 14-3-3 gamma expression can lead to various pathological conditions, underscoring 14-3-3 gamma′s importance in maintaining cellular homeostasis and signaling integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
14-3-3 γ Antibody (D-6) References:
- The 4.1/ezrin/radixin/moesin domain of the DAL-1/Protein 4.1B tumour suppressor interacts with 14-3-3 proteins. | Yu, T., et al. 2002. Biochem J. 365: 783-9. PMID: 11996670
- MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. | Lim, R., et al. 2002. J Biol Chem. 277: 40997-1008. PMID: 12176995
- The 14-3-3 cancer connection. | Hermeking, H. 2003. Nat Rev Cancer. 3: 931-43. PMID: 14737123
- Isoform-specific subcellular localization among 14-3-3 proteins in Arabidopsis seems to be driven by client interactions. | Paul, AL., et al. 2005. Mol Biol Cell. 16: 1735-43. PMID: 15659648
- p53 suppresses 14-3-3γ by stimulating proteasome-mediated 14-3-3γ protein degradation. | Chen, DY., et al. 2015. Int J Oncol. 46: 818-24. PMID: 25384678
- Emerging roles of 14-3-3γ in the brain disorder. | Cho, E. and Park, JY. 2020. BMB Rep. 53: 500-511. PMID: 32958119
- 14-3-3γ prevents centrosome duplication by inhibiting NPM1 function. | Bose, A., et al. 2021. Genes Cells. 26: 426-446. PMID: 33813791
- Prospective Role of PAK6 and 14-3-3γ as Biomarkers for Parkinson's Disease. | Giusto, E., et al. 2024. J Parkinsons Dis. 14: 495-506. PMID: 38640169
- 14-3-3: modulators of signaling proteins? | Morrison, D. 1994. Science. 266: 56-7. PMID: 7939645
- Structural organization and chromosomal assignment of the human 14-3-3 eta chain gene (YWHAH). | Muratake, T., et al. 1996. Genomics. 36: 63-9. PMID: 8812417
- The structural basis for 14-3-3:phosphopeptide binding specificity. | Yaffe, MB., et al. 1997. Cell. 91: 961-71. PMID: 9428519
- A novel sphingosine-dependent protein kinase (SDK1) specifically phosphorylates certain isoforms of 14-3-3 protein. | Megidish, T., et al. 1998. J Biol Chem. 273: 21834-45. PMID: 9705322
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
14-3-3 γ Antibody (D-6) | sc-398423 | 200 µg/ml | $322.00 | |||
14-3-3 γ Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-526311 | 200 µg Ab; 10 µg BP | $361.00 | |||
14-3-3 γ Antibody (D-6) AC | sc-398423 AC | 500 µg/ml, 25% agarose | $424.00 | |||
14-3-3 γ Antibody (D-6) HRP | sc-398423 HRP | 200 µg/ml | $322.00 | |||
14-3-3 γ Antibody (D-6) FITC | sc-398423 FITC | 200 µg/ml | $336.00 | |||
14-3-3 γ Antibody (D-6) PE | sc-398423 PE | 200 µg/ml | $349.00 | |||
14-3-3 γ Antibody (D-6) Alexa Fluor® 488 | sc-398423 AF488 | 200 µg/ml | $364.00 | |||
14-3-3 γ Antibody (D-6) Alexa Fluor® 546 | sc-398423 AF546 | 200 µg/ml | $364.00 | |||
14-3-3 γ Antibody (D-6) Alexa Fluor® 594 | sc-398423 AF594 | 200 µg/ml | $364.00 | |||
14-3-3 γ Antibody (D-6) Alexa Fluor® 647 | sc-398423 AF647 | 200 µg/ml | $364.00 | |||
14-3-3 γ Antibody (D-6) Alexa Fluor® 680 | sc-398423 AF680 | 200 µg/ml | $364.00 | |||
14-3-3 γ Antibody (D-6) Alexa Fluor® 790 | sc-398423 AF790 | 200 µg/ml | $364.00 | |||
14-3-3 γ (D-6) Neutralizing Peptide | sc-398423 P | 100 µg/0.5 ml | $69.00 |