Topo I Antibody (C-21): sc-32736

Topo I Antibody (C-21) is a mouse monoclonal IgM antibody that detects Topo I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Topo I antibody (C-21) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Topo I protein, also known as DNA topoisomerase I, plays a crucial role in maintaining DNA structure integrity during cellular processes such as replication, transcription, and recombination. By introducing a transient break in one strand of the DNA helix, Topo I alleviates torsional strain that arises ahead of the replication fork, preventing DNA supercoiling and ensuring proper DNA function. This enzymatic activity is vital for cellular health, as dysregulation of Topo I can lead to genomic instability and has been implicated in various cancers, making Topo I a significant target for anticancer therapies. Topo I interacts with several other proteins, including transcription factors and repair proteins, which further underscores Topo I′s importance in cellular processes and highlights Topo I′s potential as a therapeutic target.

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Topo I Antibody (C-21) References:

1. Topoisomerase II alpha and topoisomerase II beta genes: characterization and mapping to human chromosomes 17 and 3, respectively.  |  Tan, KB., et al. 1992. Cancer Res. 52: 231-4. PMID: 1309226
2. Structure of the human type I DNA topoisomerase gene.  |  Kunze, N., et al. 1991. J Biol Chem. 266: 9610-6. PMID: 1851751
3. Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells.  |  Austin, CA. and Fisher, LM. 1990. FEBS Lett. 266: 115-7. PMID: 2163884
4. Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes.  |  Chung, TD., et al. 1989. Proc Natl Acad Sci U S A. 86: 9431-5. PMID: 2556712
5. cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment.  |  D'Arpa, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 2543-7. PMID: 2833744
6. Discovery, Topo I inhibitory activity and mechanism evaluation of two novel cytisine-type alkaloid dimers from the seeds of Sophora alopecuroides L.  |  Meng, C., et al. 2022. Bioorg Med Chem. 61: 116723. PMID: 35396127
7. Pyridoxal hydrochloride thiosemicarbazones with copper ions inhibit cell division via Topo-I and Topo-IIɑ.  |  Qi, J., et al. 2022. J Inorg Biochem. 232: 111816. PMID: 35405490
8. Discovery of novel benzofuro[3,2-b]quinoline derivatives as dual CDK2/Topo I inhibitors.  |  Huang, Y., et al. 2022. Bioorg Chem. 126: 105870. PMID: 35636125
9. Total Synthesis of (+)-Pancratistatin and Its Potent Topo I Inhibition Activity Studies.  |  Ding, F., et al. 2022. Org Lett. 24: 9458-9462. PMID: 36522148
10. Design and synthesis of new spirooxindole candidates and their selenium nanoparticles as potential dual Topo I/II inhibitors, DNA intercalators, and apoptotic inducers.  |  El-Kalyoubi, S., et al. 2023. J Enzyme Inhib Med Chem. 38: 2242714. PMID: 37592917
11. The mechanisms of DNA topoisomerases.  |  Roca, J. 1995. Trends Biochem Sci. 20: 156-60. PMID: 7770916
12. A model for the mechanism of human topoisomerase I.  |  Stewart, L., et al. 1998. Science. 279: 1534-41. PMID: 9488652

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Topo I Antibody (C-21) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-Topo I Antibody (C-21) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Topo I Antibody (C-21) Specifications

Alternate Product Name: DNA topoisomerase I

Product Clone Name: C-21

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: Topo I

Epitope Species: human

Product Reactivity/Detection: Topo I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for Topo I Antibody (C-21): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Topo I antibody is Topo I Antibody (H-5): sc-271285.