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Smad2/3 항체 (C-8): sc-133098

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데이터 시트
  • Smad2/3 항체(C-8) 는 마우스 monoclonal IgG2aκ, 126간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human 기원의 전체 길이 TNFα-유도 단백질 2를 나타내는 아미노산 1-425에 대해 제기됨
  • 안티-Smad2/3 항체 (C-8)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Smad2 and Smad3 를 감지하는 데에 추천한다.; 이외에, canine and porcine등 species와 반응할수 있습니다
  • 항-Smad2/3 항체(C-8)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-133098 X, 200 µg/0.1 ml)이 있습니다.
  • Smad2/3 (C-8): sc-133098무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 는Smad2/3 항체(C-8) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Smad2/3 항체(C-8) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

빠른 링크

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Smad2/3 항체(C-8)는 마우스, 쥐 및 인간 유래의 Smad2 및 Smad3를 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2a κ 마우스 단일클론성 Smad2/3 항체입니다. Smad2/3 항체(C-8)는 비접합 항-Smad2/3 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-Smad2/3 항체 형태로도 사용할 수 있습니다. 초파리 모체의 포유류 상동 단백질인 Smad 단백질은 디카펜타페릭(Mad)에 대한 포유류 상동 단백질로, TGFβ/BMP 신호의 다운스트림 이펙터로 관여하고 있습니다. Smad1(Madr1 또는 JV4-1이라고도 함)과 Smad5는 BMP-2 및 BMP-4 기능의 이펙터이며, Smad2(Madr2 또는 JV18-1)와 Smad3는 TGFβ 및 Activin 매개 성장 조절에 관여합니다. Smad4(DPC4라고도 함)는 다양한 Smad 가족 구성원과의 상호작용을 통해 위의 모든 활동을 매개하는 것으로 나타났습니다. Smad6와 Smad7은 다른 Smad 단백질의 TGFβ 매개 인산화를 방해하여 Activin/TGFβ 신호에 대한 반응을 조절합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Smad2/3 항체 (C-8)

sc-133098
200 µg/ml
$316.00

Smad2/3 (C-8): m-IgG Fc BP-HRP 번들

sc-528750
200 µg Ab; 10 µg BP
$354.00

Smad2/3 (C-8): m-IgGκ BP-HRP 번들

sc-521235
200 µg Ab, 40 µg BP
$354.00

Smad2/3 항체 (C-8) X

sc-133098 X
200 µg/0.1 ml
$316.00

Smad2/3 항체 (C-8) AC

sc-133098 AC
500 µg/ml, 25% agarose
$416.00

Smad2/3 항체 (C-8) HRP

sc-133098 HRP
200 µg/ml
$316.00

Smad2/3 항체 (C-8) FITC

sc-133098 FITC
200 µg/ml
$330.00

Smad2/3 항체 (C-8) PE

sc-133098 PE
200 µg/ml
$343.00

Smad2/3 항체 (C-8) Alexa Fluor® 488

sc-133098 AF488
200 µg/ml
$357.00

Smad2/3 항체 (C-8) Alexa Fluor® 546

sc-133098 AF546
200 µg/ml
$357.00

Smad2/3 항체 (C-8) Alexa Fluor® 594

sc-133098 AF594
200 µg/ml
$357.00

Smad2/3 항체 (C-8) Alexa Fluor® 647

sc-133098 AF647
200 µg/ml
$357.00

Smad2/3 항체 (C-8) Alexa Fluor® 680

sc-133098 AF680
200 µg/ml
$357.00

Smad2/3 항체 (C-8) Alexa Fluor® 790

sc-133098 AF790
200 µg/ml
$357.00

I want to detect SMAD2/3 phosphorylated protein expression in porcine cells. But I'm not found this criteria in primary antibody of Santa cruz. I found that p-Smad2/ 3 (Ser 423/425) is not available now. Can you suggest me which one is proper?

Asked by: Ploy
Thank you for your question. Unfortunately we do not have a anti-phosphorylated SMAD antibody at this time. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-20

I WOULD LIKE TO PURCHASE ANTIBODY AGAINST PHOSPHORLYATED SMAD1 AN SMAD2 AND THEIR SECONDARY ANTIBODY FOR WESTERN BLOT

Asked by: MM ALHARBI
Thank you for your question. Unfortunately, we do not currently offer any phospho-specific Smad1 or Smad2 antibodies. This mouse monoclonal, Smad2/3 Antibody (C-8): sc-133098, is against total Smad2/3 and may detect both the phosphorylated and non-phosphorylated forms of the proteins.
Answered by: Technical Support
Date published: 2017-08-22

Can Smad2/3 (C-8): sc-133098 monoclonal antibody be used for detection of Smad2/3 of equine origin?

Asked by: jenni
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 14
Date published: 2017-01-11
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Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:200 for overnight @ 4oC.
Date published: 2024-12-10
Rated 5 out of 5 by from Smad 2/3I tested this antibody in western blotting (1:200) and I am very satisfied.
Date published: 2024-01-26
Rated 5 out of 5 by from This antibody is very good!The bands bound with this antibody are very well exposed. I purchased this antibody last year and performed western blot detection on the cell protein samples collected from the RIPA lysate, and the background was very clean.
Date published: 2022-10-08
Rated 5 out of 5 by from The antibody was very goodWhen I use the antibody, the bands were easy to visualize and very clear.
Date published: 2022-07-29
Rated 4 out of 5 by from Good in immunofluorescenceGood resuts have been achieved in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA.
Date published: 2020-11-10
Rated 4 out of 5 by from Buena marcaObtuve buena marca, debo corregir el inespecifico o diluir el acpo
Date published: 2019-03-14
Rated 5 out of 5 by from Nice and strong band (w.b.)Produced a single band at the expected molecular weight (~55kDa). Sample: MDA-MB-231 wcl (30ug). Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer, overnight @ 4oC. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 5 seconds* I'm certainly very satisfied with this product. *further dilution of the primary antibody, maybe even at 1:1000, will likely yield similar results at the expense of a prolonged (and more adequate) exposure time.
Date published: 2018-11-30
Rated 5 out of 5 by from my review for Smad2/3 antibody (sc-133098)I tested a free antibody for Smad2/3 antibody (sc-133098) using IP samples from mouse fibrotic kidneys for Western blot. We loaded 80 ug proteins in each slot in the SDS gel. The antibody was diluted in 1:500 in 3% BSA/PBST and incubated overnight with the blot. We can observe a very clear band around 55-60 kd. We have repeated this experiment several times and used the fresh diluted antibody on the fresh blot.
Date published: 2018-05-14
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Smad2/3 항체 (C-8) is rated 4.8 out of 5 by 42.
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