IP3R-I/II/III 항체 (B-2) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 IP3R-I, IP3R-II and IP3R-III을 검출할것을 권장합니다.

IP3R-I/II/III 항체 (B-2): sc-377518

IP3R-I/II/III 항체 (B-2) is rated 4.2 out of 5 by 12.
  • y_2021, m_9, d_22, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_ko_KR, sid_377518, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 87ms
  • REVIEWS, PRODUCT
loading
5
9
4
0
3
1
2
0
1
2

데이터 시트

    • 안티-IP3R-I/II/III 항체 B-2 는 마우스 monoclonal IgG2b IP3R-I/II/III 항체, 12간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • human origin의 IP3R-II을 항원으로 사용하였습니다.
    • 안티-IP3R-I/II/III 항체 (B-2)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 IP3R-I, IP3R-II and IP3R-III 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-IP3R-I/II/III 항체 (B-2)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • IP3R-I/II/III (B-2): sc-377518무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for IP3R-I/II/III Antibody (B-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with IP3R-I/II/III Antibody (B-2) (see ordering information below).
loading

IP3R-I/II/III 抗体 (B-2): sc-377518此抗体是ITPR1/2/3这三型蛋白都能结合么?

Asked by: marynaxj
感谢您的提问。都能检测的。更多问题,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. Yes, recommended for detection of IP3R-I, IP3R-II and IP3R-III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-10-30

Is antigen retrieval necessary when using this antibody in IHC(P)?

Asked by: AbPolly
Thank you for your question. We do recommend antigen retrieval when working with paraffin-embedded sections, specifically sodium citrate buffer, pH 6.0, and heat. Our full protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service
Date published: 2017-02-03
  • y_2021, m_9, d_22, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_ko_KR, sid_377518, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 126ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from Works, but not super strong Used in H9 hESC and HEK293T lysates to optimise this and other antibodies. Used at 1:1000 dilution in 5% milk in TBST for 24hrs at 4C.
Date published: 2021-08-24
Rated 1 out of 5 by from No signal in WB I tested this antibody on mouse and human whole cell lysates and no signal was detected.
Date published: 2020-11-18
Rated 1 out of 5 by from This antibody does not recognize IP3R-III We tried this antibody using lysates from cells expressing R1 only, R2 only, R3 only. Conclusion: this antibody recognized R2 very strongly but did not recognize R3 at all ( Human R3 or rat R3). Also, it recognized over-expressed R1 very very weakly. Therefore, it is not a pan-antibody.
Date published: 2017-09-21
Rated 5 out of 5 by from Very good IP3R antibody. I tested in WB and IF. very good IP3R antibody. I tested in WB(1:500) and IF(1:50),it works very well!
Date published: 2017-08-26
Rated 5 out of 5 by from clean blot seen clean blot seen,you can tried 1:400 to 1:1000 dilution,this conceration is enough detect IP3R-I/II/III ,the target at above250kda, recommend use 6% SDS-PAGE.
Date published: 2017-06-25
Rated 5 out of 5 by from A good antibody We use this as a loading control for WB, It always very good quality
Date published: 2017-06-01
Rated 5 out of 5 by from reommend use This antibody gave strong and clear detection of IP3R-I/II/III , allowing us to trace translocation from the cytoplasm to nucleus by IF with a standard secondary antibody.
Date published: 2017-02-05
Rated 5 out of 5 by from Good IP3R-I/II/III antibody Strong staining observed in IHC(P) and IF, and strong signal obtained in WB with endogenous and transfected samples. Great antibody for many applications.
Date published: 2017-01-30
  • y_2021, m_9, d_22, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_ko_KR, sid_377518, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT