mouse origin의 Id3에서 내부에 위치한 1-119 아미노산을 항원으로 사용하였습니다.
안티-Id3 항체 (2B11)는 WB, IP and IF으로 mouse, rat and human유래의 Id3 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Id3 항체 (2B11)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Id3 Antibody (2B11) is a high quality monoclonal Id3 antibody (also designated DNA-binding protein inhibitor ID-3 antibody, ID3 antibody, or HEIR1 antibody) suitable for the detection of the Id3 protein of mouse, rat and human origin. Id3 Antibody (2B11) is available as both the non-conjugated anti-Id3 antibody form, as well as multiple conjugated forms of anti-Id3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can Id3 (2B11): sc-56712 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Id3 (2B11): sc-56712 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Rated 4 out of
Emilia Du from
WB 效果不错申请的10uL试用装，1:200 WB效果不错，只是回收二次利用效果不好，希望增大稀释比例，免疫荧光等尚未使用
Date published: 2018-03-05
Rated 2 out of
detects only recombinant protein in WBIn lung cancer cell lines only recombinant fusion protein was detectable in WB at a dilution of 1:100 in TBST, 5%BSA with some overall background signal and also background signal at the expected protein size.