Id1 항체 (B-8) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 Id1을 검출할것을 권장합니다.

Id1 항체 (B-8): sc-133104

Id1 항체 (B-8) is rated 4.9 out of 5 by 10.
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데이터 시트

    • 안티-Id1 항체 B-8 는 마우스 monoclonal IgG2a κ Id1 항체, 25간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 mouse origin의 Id1의 내부에 위치한 118-159의 아미노산에 해당됩니다.
    • 안티-Id1 항체 (B-8)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Id1 를 감지하는 데에 추천한다.; 이외에 canine등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Id1 항체 (B-8)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-133104 X, 200 µg/0.1 ml)이 있습니다.
    • Id1 (B-8): sc-133104무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Id1 Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Id1 Antibody (B-8) (see ordering information below).

How much protein do you recommend loading on the gel for Western blot detection using Id1 (B-8): sc-133104 monoclonal antibody?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 4 out of 5 by from IF效果很好 特异性高,IF 1:200背景干净,比做WB效果好,WB 1:500稀释无杂带,但抗体回收后二次利用效果不好。
Date published: 2018-03-05
Rated 5 out of 5 by from The antibody works well Positive result was found in the tubular region of human kidney tissues.
Date published: 2017-11-27
Rated 5 out of 5 by from 功能很出色! 特异性高,效果好,无杂带,抗体很稳定,重复性好,非常好用。建议是否可以放大稀释倍数?希望能够多推出试用装抗体
Date published: 2017-10-31
Rated 5 out of 5 by from Great The antibody is pretty good for IF staining. The background is very low.
Date published: 2017-08-02
Rated 5 out of 5 by from 抗体条带单一 内皮细胞WB检测ID1的表达,条带单一,,用的1:1000稀释的抗体,如果1:100稀释抗体效果可能会更好些。
Date published: 2017-07-11
Rated 5 out of 5 by from Great Antibody Antibody produced robust signal by WB, IF and IHC(P). Highly recommended!
Date published: 2017-01-06
Rated 5 out of 5 by from Great immunofluorescence nuclear staining Great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Positive band see in WB in Ramos and HeLa Positive band see in WB in Ramos and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-24
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