항원결정부는 mouse origin의 Id1의 내부에 위치한 118-159의 아미노산에 해당됩니다.
안티-Id1 항체 (B-8)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Id1 를 감지하는 데에 추천한다.; 이외에 canine등 species와 반응할수 있습니다
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Id1 항체 (B-8)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-133104 X, 200 µg/0.1 ml)이 있습니다.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Id1 Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Id1 Antibody (B-8) (see ordering information below).
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Id1 Antibody (B-8) is a high quality monoclonal Id1 antibody (also designated Inhibitor of DNA binding 1 antibody, ID1 antibody or bHLHb24 antibody) suitable for the detection of the Id1 protein of mouse, rat and human origin. Id1 Antibody (B-8) is available as both the non-conjugated anti-Id1 antibody form, as well as multiple conjugated forms of anti-Id1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
How much protein do you recommend loading on the gel for Western blot detection using Id1 (B-8): sc-133104 monoclonal antibody?
Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference.