14-3-3 ζ Antibody (G-2): sc-518031
- 14-3-3 ζ Antibody (G-2) is a mouse monoclonal IgG3 κ, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 122-140 within an internal region of 14-3-3 ζ of human origin
- Anti-14-3-3 zeta Antibody (G-2) is recommended for detection of 14-3-3 ζ and, to a lesser extent, 14-3-3 ε of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-14-3-3 zeta Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG3 BP-HRP is the preferred secondary detection reagent for 14-3-3 ζ Antibody (G-2) for WB applications. This reagent is now offered in a bundle with 14-3-3 ζ Antibody (G-2) (see ordering information below).
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14-3-3 ζ Antibody (G-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects 14-3-3 ζ protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-14-3-3 ζ antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 14-3-3 ζ protein plays a crucial role in regulating cellular processes such as apoptosis, mitogenic signaling, and cell-cycle checkpoints, which are particularly relevant in cancer biology. As part of a family of seven isoforms, including 14-3-3 β, γ, ε, ζ, η, θ, and σ, 14-3-3 proteins function by forming dimers that create two binding sites for ligand proteins, facilitating interactions that may not occur otherwise. These proteins primarily recognize a consensus binding motif characterized by serine/threonine phosphorylation, allowing them to regulate a wide array of cellular functions. 14-3-3 ζ protein′s ability to sequester proteins in the cytoplasm, occupy interaction domains, and modulate enzymatic activity underscores its importance in maintaining cellular homeostasis. Disruption of 14-3-3 ζ expression can lead to various pathological conditions, highlighting its significance in cellular signaling pathways and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
14-3-3 ζ Antibody (G-2) References:
- Proteomic analysis of 14-3-3 zeta binding proteins in the mouse hippocampus. | Heverin, M., et al. 2012. Int J Physiol Pathophysiol Pharmacol. 4: 74-83. PMID: 22837806
- Characterization of 14-3-3-ζ Interactions with integrin tails. | Bonet, R., et al. 2013. J Mol Biol. 425: 3060-72. PMID: 23763993
- Expression profiles for 14-3-3 zeta and CCL20 in pancreatic cancer and chronic pancreatitis. | Klemm, C., et al. 2014. Pathol Res Pract. 210: 335-41. PMID: 24629487
- 14-3-3-zeta participates in TLR3-mediated TICAM-1 signal-platform formation. | Funami, K., et al. 2016. Mol Immunol. 73: 60-8. PMID: 27058640
- 14-3-3 α and 14-3-3 ζ contribute to immune responses in planarian Dugesia japonica. | Lu, Q., et al. 2017. Gene. 615: 25-34. PMID: 28322998
- Expression of 14-3-3 Zeta Protein in Dexamethasone-Treated Mice and Human TM-1 Cells. | Liu, Q., et al. 2017. Curr Eye Res. 42: 1124-1129. PMID: 28494163
- Down-regulation of 14-3-3 zeta sensitizes human glioblastoma cells to apoptosis induction. | Hashemi, M., et al. 2018. Apoptosis. 23: 616-625. PMID: 30101359
- 14-3-3-zeta mediates GLP-1 receptor agonist action to alter α cell proglucagon processing. | Holter, MM., et al. 2022. Sci Adv. 8: eabn3773. PMID: 35867787
- A possible mechanism to the antidepressant-like effects of 20 (S)-protopanaxadiol based on its target protein 14-3-3 ζ. | Chen, L., et al. 2022. J Ginseng Res. 46: 666-674. PMID: 36090685
- Molecular Characterization of 14-3-3 Zeta Gene in Musca domestica (Diptera: Muscidae) and Its Roles in Response to Bacterial Infection. | Jiao, Z., et al. 2022. J Insect Sci. 22: PMID: 36315471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
14-3-3 ζ Antibody (G-2) | sc-518031 | 200 µg/ml | $322.00 | |||
14-3-3 ζ Antibody (G-2): m-IgG3 BP-HRP Bundle | sc-552969 | 200 µg Ab; 40 µg BP | $361.00 | |||
14-3-3 ζ Antibody (G-2) AC | sc-518031 AC | 500 µg/ml, 25% agarose | $424.00 | |||
14-3-3 ζ Antibody (G-2) HRP | sc-518031 HRP | 200 µg/ml | $322.00 | |||
14-3-3 ζ Antibody (G-2) FITC | sc-518031 FITC | 200 µg/ml | $336.00 | |||
14-3-3 ζ Antibody (G-2) PE | sc-518031 PE | 200 µg/ml | $349.00 | |||
14-3-3 ζ Antibody (G-2) Alexa Fluor® 488 | sc-518031 AF488 | 200 µg/ml | $364.00 | |||
14-3-3 ζ Antibody (G-2) Alexa Fluor® 546 | sc-518031 AF546 | 200 µg/ml | $364.00 | |||
14-3-3 ζ Antibody (G-2) Alexa Fluor® 594 | sc-518031 AF594 | 200 µg/ml | $364.00 | |||
14-3-3 ζ Antibody (G-2) Alexa Fluor® 647 | sc-518031 AF647 | 200 µg/ml | $364.00 | |||
14-3-3 ζ Antibody (G-2) Alexa Fluor® 680 | sc-518031 AF680 | 200 µg/ml | $364.00 | |||
14-3-3 ζ Antibody (G-2) Alexa Fluor® 790 | sc-518031 AF790 | 200 µg/ml | $364.00 |