Ref-1 Antibody (C-4): sc-17774

Ref-1 Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ref-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-Ref-1 antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ref-1 protein, also known as apurinic/apyrimidinic endodeoxyribonuclease 1, plays a crucial role in DNA repair and redox regulation of transcription factors, which is vital for maintaining cellular homeostasis and responding to oxidative stress. Located primarily in the nucleus, Ref-1 protein is essential for the repair of apurinic/apyrimidinic sites in DNA, which can arise from oxidative damage or spontaneous hydrolysis. This repair function is critical because unrepaired DNA lesions can lead to mutations, genomic instability, and contribute to the development of various diseases, including cancer. Additionally, Ref-1′s redox modulation of transcription factors enhances DNA binding activity, thereby influencing gene expression in response to cellular stress. The interaction between Ref-1 protein and other proteins, such as Fos and Jun, highlights Ref-1′s importance in cellular signaling pathways and gene regulation, making Ref-1 (C-4) antibody a valuable tool for researchers studying these critical biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ref-1 Antibody (C-4) References:

1. Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme.  |  Xanthoudakis, S., et al. 1992. EMBO J. 11: 3323-35. PMID: 1380454
2. Identification and characterization of Ref-1, a nuclear protein that facilitates AP-1 DNA-binding activity.  |  Xanthoudakis, S. and Curran, T. 1992. EMBO J. 11: 653-65. PMID: 1537340
3. Reduction of a conserved Cys is essential for Myb DNA-binding.  |  Guehmann, S., et al. 1992. Nucleic Acids Res. 20: 2279-86. PMID: 1594446
4. The regulation of transcription by phosphorylation.  |  Hunter, T. and Karin, M. 1992. Cell. 70: 375-87. PMID: 1643656
5. Activation of protein kinase C decreases phosphorylation of c-Jun at sites that negatively regulate its DNA-binding activity.  |  Boyle, WJ., et al. 1991. Cell. 64: 573-84. PMID: 1846781
6. Redox regulation of fos and jun DNA-binding activity in vitro.  |  Abate, C., et al. 1990. Science. 249: 1157-61. PMID: 2118682
7. APE1/Ref-1 Role in Inflammation and Immune Response.  |  Oliveira, TT., et al. 2022. Front Immunol. 13: 793096. PMID: 35296074
8. APE1/Ref-1 as a Therapeutic Target for Inflammatory Bowel Disease.  |  Sahakian, L., et al. 2023. Biomolecules. 13: PMID: 38002251
9. The APE1/REF-1 and the hallmarks of cancer.  |  Siqueira, PB., et al. 2024. Mol Biol Rep. 51: 47. PMID: 38165468
10. Peptide REF1 is a local wound signal promoting plant regeneration.  |  Yang, W., et al. 2024. Cell. 187: 3024-3038.e14. PMID: 38781969
11. The redox and DNA-repair activities of Ref-1 are encoded by nonoverlapping domains.  |  Xanthoudakis, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 23-7. PMID: 7506414
12. Identification of residues in the human DNA repair enzyme HAP1 (Ref-1) that are essential for redox regulation of Jun DNA binding.  |  Walker, LJ., et al. 1993. Mol Cell Biol. 13: 5370-6. PMID: 8355688

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ref-1 Antibody (C-4) has 63 citations in a variety of scientific publications.

Primary antibodies like Anti-Ref-1 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ref-1 Antibody (C-4) Specifications

Alternate Product Name: APEX1

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: Ref-1

Epitope Species: human

Product Reactivity/Detection: Ref-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 63 citations in various scientific publications

Available Conjugates/Forms for Ref-1 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent