PDE7A1/3 Antibody (F-5): sc-271324

PDE7A1/3 Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDE7A1/3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDE7A1/3 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDE7A1/3 protein plays a crucial role in regulating intracellular levels of cyclic adenosine monophosphate (cAMP), a second messenger vital for numerous cellular processes, including signal transduction pathways that influence cell growth, metabolism, and apoptosis. By hydrolyzing cAMP to 5′AMP, phosphodiesterases like PDE7A1/3 help maintain cellular homeostasis and prevent excessive signaling that could lead to pathological conditions. Notably, PDE7A1 isozyme is highly expressed in skeletal muscle, where PDE7A1 contributes to muscle function and metabolism, while other isoforms such as PDE3A and PDE3B are primarily found in cardiovascular and adipose tissues, respectively. Understanding the specific roles of these phosphodiesterases is essential for developing targeted therapies for diseases related to cAMP dysregulation, making anti-PDE7A1/3 antibody (F-5) a valuable tool for researchers studying these critical pathways.

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PDE7A1/3 Antibody (F-5) References:

1. Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase.  |  Hetman, JM., et al. 2000. Proc Natl Acad Sci U S A. 97: 472-6. PMID: 10618442
2. Cardiac type cGMP-inhibited phosphodiesterase (PDE3A) gene structure: similarity and difference to adipocyte type PDE3B gene.  |  Kasuya, J., et al. 2000. Biochem Biophys Res Commun. 268: 827-34. PMID: 10679291
3. 5-Imino-1,2-4-thiadiazoles and quinazolines derivatives as glycogen synthase kinase 3β (GSK-3β) and phosphodiesterase 7 (PDE7) inhibitors: determination of blood-brain barrier penetration and binding to human serum albumin.  |  Pérez, DI., et al. 2012. Eur J Pharm Sci. 45: 677-84. PMID: 22306656
4. Effect of phosphodiesterase 7 (PDE7) inhibitors in experimental autoimmune encephalomyelitis mice. Discovery of a new chemically diverse family of compounds.  |  Redondo, M., et al. 2012. J Med Chem. 55: 3274-84. PMID: 22385507
5. Neuronal lack of PDE7a disrupted working memory, spatial learning, and memory but facilitated cued fear memory in mice.  |  Liu, Z., et al. 2023. Prog Neuropsychopharmacol Biol Psychiatry. 120: 110655. PMID: 36220621
6. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants.  |  Bloom, TJ. and Beavo, JA. 1996. Proc Natl Acad Sci U S A. 93: 14188-92. PMID: 8943082
7. Alternative splicing of the high affinity cAMP-specific phosphodiesterase (PDE7A) mRNA in human skeletal muscle and heart.  |  Han, P., et al. 1997. J Biol Chem. 272: 16152-7. PMID: 9195912
8. Cyclic AMP phosphodiesterases in human lymphocytes.  |  Sheth, SB., et al. 1997. Br J Haematol. 99: 784-9. PMID: 9432022
9. Phosphodiesterase profile of human B lymphocytes from normal and atopic donors and the effects of PDE inhibition on B cell proliferation.  |  Gantner, F., et al. 1998. Br J Pharmacol. 123: 1031-8. PMID: 9559883
10. Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase.  |  Fisher, DA., et al. 1998. Biochem Biophys Res Commun. 246: 570-7. PMID: 9618252
11. Partial characterization of the active site human platelet cAMP phosphodiesterase, PDE3A, by site-directed mutagenesis.  |  Cheung, PP., et al. 1998. Arch Biochem Biophys. 360: 99-104. PMID: 9826434
12. Expression of cyclic GMP-inhibited phosphodiesterases 3A and 3B (PDE3A and PDE3B) in rat tissues: differential subcellular localization and regulated expression by cyclic AMP.  |  Liu, H. and Maurice, DH. 1998. Br J Pharmacol. 125: 1501-10. PMID: 9884079

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDE7A1/3 Antibody (F-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PDE7A1/3 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDE7A1/3 Antibody (F-5) Specifications

Alternate Product Name: PDE7A1/3

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-50

Epitope Target: PDE7A1

Epitope Species: human

Product Reactivity/Detection: PDE7A1 and PDE7A3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PDE7A1/3 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PDE7A1/2 antibody is PDE7A1/2 Antibody (H-7): sc-28374.