Lad Antibody (H-3): sc-515932. Western blot analysis of Lad expression in non-transfected: sc-117752 (A) and human Lad transfected: sc-177448 (B) whole cell lysates.
Lad Antibody (H-3): sc-515932
- Lad Antibody (H-3) is a mouse monoclonal IgG2a κ Lad antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 296-322 near the C-terminus of Lad of mouse origin
- Lad Antibody (H-3) is recommended for detection of Lad of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Lad Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Lad Antibody (H-3) for WB applications. These reagents are now offered in bundles with Lad Antibody (H-3) (see ordering information below).
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SEE ALSO...
Lad (H-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Lad protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-Lad antibody (H-3) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Lad (TSAD, TSAd, F2771, SH2D2A, SH2 domain protein 2A) is a cytoplasmic adapter protein that undergoes tyrosine-phosphorylation and influences T cell activation. Lad (SH2D2A) mRNA is present in peripheral blood leukocytes, thymus and spleen, and accumulates in the cytoplasm during T cell activation. Lad gene maps to chromosome 1q21 in a region where alterations are characteristic to lymphomas.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Lad Antibody (H-3) References:
- Lad, an adapter protein interacting with the SH2 domain of p56lck, is required for T cell activation. | Choi, YB., et al. 1999. J Immunol. 163: 5242-9. PMID: 10553045
- The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene cluster on human Chromosome 1. | Dai, KZ., et al. 2000. Immunogenetics. 51: 179-85. PMID: 10752626
- The T cell regulator gene SH2D2A contributes to the genetic susceptibility of multiple sclerosis. | Dai, KZ., et al. 2001. Genes Immun. 2: 263-8. PMID: 11528519
- Impaired T cell death and lupus-like autoimmunity in T cell-specific adapter protein-deficient mice. | Drappa, J., et al. 2003. J Exp Med. 198: 809-21. PMID: 12953096
- Transcriptional activation of the SH2D2A gene is dependent on a cyclic adenosine 5'-monophosphate-responsive element in the proximal SH2D2A promoter. | Dai, KZ., et al. 2004. J Immunol. 172: 6144-51. PMID: 15128801
- cDNA cloning of a rat orthologue of SH2D2A encoding T-cell-specific adaptor protein (TSAd): expression in T and NK cells. | Nejad, S., et al. 2004. Immunogenetics. 56: 338-42. PMID: 15300336
- Molecular cloning of a T cell-specific adapter protein (TSAd) containing an Src homology (SH) 2 domain and putative SH3 and phosphotyrosine binding sites. | Spurkland, A., et al. 1998. J Biol Chem. 273: 4539-46. PMID: 9468509
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lad Antibody (H-3) | sc-515932 | 200 µg/ml | $322.00 | |||
Lad Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-531563 | 200 µg Ab; 10 µg BP | $361.00 | |||
Lad Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-525288 | 200 µg Ab, 40 µg BP | $361.00 | |||
Lad Antibody (H-3): m-IgG2a BP-HRP Bundle | sc-547819 | 200 µg Ab; 10 µg BP | $361.00 | |||
Lad Antibody (H-3) AC | sc-515932 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Lad Antibody (H-3) HRP | sc-515932 HRP | 200 µg/ml | $322.00 | |||
Lad Antibody (H-3) FITC | sc-515932 FITC | 200 µg/ml | $336.00 | |||
Lad Antibody (H-3) PE | sc-515932 PE | 200 µg/ml | $349.00 | |||
Lad Antibody (H-3) Alexa Fluor® 488 | sc-515932 AF488 | 200 µg/ml | $364.00 | |||
Lad Antibody (H-3) Alexa Fluor® 546 | sc-515932 AF546 | 200 µg/ml | $364.00 | |||
Lad Antibody (H-3) Alexa Fluor® 594 | sc-515932 AF594 | 200 µg/ml | $364.00 | |||
Lad Antibody (H-3) Alexa Fluor® 647 | sc-515932 AF647 | 200 µg/ml | $364.00 | |||
Lad Antibody (H-3) Alexa Fluor® 680 | sc-515932 AF680 | 200 µg/ml | $364.00 | |||
Lad Antibody (H-3) Alexa Fluor® 790 | sc-515932 AF790 | 200 µg/ml | $364.00 |