IRS-4 Antibody (RI-7): sc-100854

IRS-4 Antibody (RI-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects IRS-4 of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-IRS-4 antibody (RI-7) is available in a non-conjugated form, providing flexibility for various experimental needs. Insulin receptor substrate (IRS) proteins play a crucial role in mediating signaling pathways initiated by the Insulin receptor, and IRS-4, as the most recently identified member of this family, is particularly significant due to involvement in metabolic regulation. IRS-4 is primarily located in the cytoplasm and can translocate to the nucleus under certain conditions, which is essential for its role in insulin signaling and gene expression regulation. IRS-4′s ability to interact with phosphatidylinositol 3-kinase (PI3-kinase) upon phosphorylation highlights importance in insulin-stimulated DNA synthesis and cellular growth processes. IRS-4′s interaction with IRAS, which enhances IRS-4-dependent stimulation of PI3-kinase when overexpressed, underscores potential implications in metabolic disorders. Expression of IRS-4 is notably limited to specific fiber types in heart and skeletal muscle tissues, and absence in mice has been linked to mild defects in growth, reproduction, and glucose homeostasis, indicating a vital role in maintaining metabolic balance. Overexpression of IRS-4 has been associated with increased basal PI3-kinase activity and Akt phosphorylation, further emphasizing importance in cellular signaling pathways.

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IRS-4 Antibody (RI-7) References:

1. The insulin receptor substrate 4 gene (IRS4) is mutated in paediatric T-cell acute lymphoblastic leukaemia.  |  Karrman, K., et al. 2011. Br J Haematol. 155: 516-9. PMID: 21517825
2. Ankyrin repeat and SOCS box containing protein 4 (Asb-4) colocalizes with insulin receptor substrate 4 (IRS4) in the hypothalamic neurons and mediates IRS4 degradation.  |  Li, JY., et al. 2011. BMC Neurosci. 12: 95. PMID: 21955513
3. IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation.  |  Ikink, GJ., et al. 2016. Nat Commun. 7: 13567. PMID: 27876799
4. Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression.  |  Cui, A., et al. 2017. Tumour Biol. 39: 1010428317701640. PMID: 28475006
5. Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway.  |  Li, X., et al. 2018. Theranostics. 8: 3643-3653. PMID: 30026872
6. IRS4 promotes the progression of non-small cell lung cancer and confers resistance to EGFR-TKI through the activation of PI3K/Akt and Ras-MAPK pathways.  |  Hao, P., et al. 2021. Exp Cell Res. 403: 112615. PMID: 33894221
7. PD-L1 Triggered by Binding eIF3I Contributes to the Amelioration of Diabetes-Associated Wound Healing Defects by Regulating IRS4.  |  Kuai, L., et al. 2022. J Invest Dermatol. 142: 220-231.e8. PMID: 34293353
8. FER-mediated phosphorylation and PIK3R2 recruitment on IRS4 promotes AKT activation and tumorigenesis in ovarian cancer cells.  |  Zhang, Y., et al. 2022. Elife. 11: PMID: 35550247
9. HDAC inhibitors target IRS4 to enhance anti‑AR therapy in AR‑positive triple‑negative breast cancer.  |  He, Y., et al. 2024. Int J Oncol. 64: PMID: 38214343
10. Evodiamine inhibits growth of vemurafenib drug-resistant melanoma via suppressing IRS4/PI3K/AKT signaling pathway.  |  Guo, X., et al. 2024. J Nat Med. 78: 342-354. PMID: 38324123

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRS-4 Antibody (RI-7) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-IRS-4 Antibody (RI-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRS-4 Antibody (RI-7) Specifications

Alternate Product Name: IRS-4

Product Clone Name: RI-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 850-951

Epitope Target: IRS-4

Epitope Species: human

Product Reactivity/Detection: IRS-4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for IRS-4 Antibody (RI-7): N/A

The preferred IRS-4 antibody is IRS-4 Antibody (C-1): sc-373778.