HSF1 Antibody (E-4): sc-17757

HSF1 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSF1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). HSF1 monoclonal antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. HSF1 plays a crucial role in cellular response to stress, particularly thermal and chemical stress, by regulating the expression of heat shock proteins that help protect cells from damage. This regulation is vital for maintaining cellular homeostasis and promoting cell survival under adverse conditions. HSF1 is typically present as a monomer, but upon activation, forms trimers that translocate to the nucleus, where HSF1 binds to heat shock elements in promoter regions of target genes, facilitating transcription. HSF1 is upregulated by estrogen, which enhances activity at both mRNA and protein levels, further underscoring importance in cellular stress responses and potential implications in diseases related to stress and inflammation.

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HSF1 Antibody (E-4) References:

1. Transcriptional activation of heat-shock genes in eukaryotes.  |  Tanguay, RM. 1988. Biochem Cell Biol. 66: 584-93. PMID: 3048332
2. The Role of HSF1 Protein in Malignant Transformation.  |  Šimončík, O., et al. 2018. Klin Onkol. 31: 55-62. PMID: 31023025
3. The Role of HSF1 and the Chaperone Network in the Tumor Microenvironment.  |  Grunberg, N., et al. 2020. Adv Exp Med Biol. 1243: 101-111. PMID: 32297214
4. HSF1 is involved in immunotherapeutic response through regulating APOJ/STAT3-mediated PD-L1 expression in hepatocellular carcinoma.  |  Cheng, H., et al. 2023. Cancer Biol Ther. 24: 1-9. PMID: 36482717
5. Heat shock factor 1 (HSF1) specifically potentiates c-MYC-mediated transcription independently of the canonical heat shock response.  |  Xu, M., et al. 2023. Cell Rep. 42: 112557. PMID: 37224019
6. Transcriptional responses of cancer cells to heat shock-inducing stimuli involve amplification of robust HSF1 binding.  |  Dastidar, SG., et al. 2023. Nat Commun. 14: 7420. PMID: 37973875
7. Estrogen dependent expression of heat shock transcription factor: implications for uterine synthesis of heat shock proteins.  |  Yang, X., et al. 1995. J Steroid Biochem Mol Biol. 52: 415-9. PMID: 7748806
8. Determination of heat-shock transcription factor 2 stoichiometry at looped DNA complexes using scanning force microscopy.  |  Wyman, C., et al. 1995. EMBO J. 14: 117-23. PMID: 7828583
9. Function and regulation of heat shock factor 2 during mouse embryogenesis.  |  Rallu, M., et al. 1997. Proc Natl Acad Sci U S A. 94: 2392-7. PMID: 9122205
10. Heat shock response and protein degradation: regulation of HSF2 by the ubiquitin-proteasome pathway.  |  Mathew, A., et al. 1998. Mol Cell Biol. 18: 5091-8. PMID: 9710593
11. Proteasome inhibition leads to the activation of all members of the heat-shock-factor family.  |  Kawazoe, Y., et al. 1998. Eur J Biochem. 255: 356-62. PMID: 9716376
12. Glycogen synthase kinase 3beta and extracellular signal-regulated kinase inactivate heat shock transcription factor 1 by facilitating the disappearance of transcriptionally active granules after heat shock.  |  He, B., et al. 1998. Mol Cell Biol. 18: 6624-33. PMID: 9774677

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSF1 Antibody (E-4) has 51 citations in a variety of scientific publications.

Primary antibodies like Anti-HSF1 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSF1 Antibody (E-4) Specifications

Alternate Product Name: HSF1

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 219-529

Epitope Target: HSF1

Epitope Species: human

Product Reactivity/Detection: HSF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 51 citations in various scientific publications

Available Conjugates/Forms for HSF1 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent