CD3-ε Antibody (H-12): sc-137234

CD3-ε Antibody (H-12) is a mouse monoclonal IgG1 antibody that detects CD3-ε in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CD3-ε is a crucial component of the T cell receptor (TCR) complex, playing a vital role in T cell activation and signaling. CD3-ε (H-12) antibody forms a heterodimer with CD3-γ and is essential for the transduction of activation signals upon antigen recognition. CD3-ε expression on the T cell surface is critical for proper immune response functioning, as CD3-ε helps initiate the signaling cascade that leads to T cell proliferation and differentiation. Notably, CD3-ε contains an immunoreceptor tyrosine-based activation motif (ITAM) that is pivotal for recruiting protein kinases, such as ZAP-70, which further propagate the activation signal within the cell. The interaction of CD3-ε with these kinases underscores CD3-ε′s importance in the immune system, as CD3-ε ensures that T cells can effectively respond to pathogens. Anti-CD3-ε antibody (H-12) is therefore an invaluable tool for researchers studying T cell biology and the mechanisms of immune activation.

The preferred CD3-ε antibody is CD3-ε Antibody (UCH-T1): sc-1179.