Bak Antibody (G-10): sc-518146

Bak Antibody (G-10) is a mouse monoclonal IgM kappa antibody that detects Bak protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Bak (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bak protein, a member of the Bcl-2 family, plays a crucial role in the regulation of apoptosis, particularly in promoting cell death in response to specific signals. Bak′s ability to enhance apoptotic processes is vital for maintaining cellular homeostasis and eliminating damaged or unwanted cells, which is essential for tissue health and development. Bak is predominantly located in the mitochondrial membrane, where Bak interacts with other pro-apoptotic and anti-apoptotic proteins, thereby influencing the intrinsic pathway of apoptosis. Regulation of Bak′s activity is critical, as Bak overexpression can lead to excessive cell death, while Bak inhibition can contribute to tumorigenesis. Understanding the dynamics of Bak and Bak interactions with other proteins is fundamental for developing therapeutic strategies targeting apoptosis in various diseases, including cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bak Antibody (G-10) References:

1. Physiological and Pharmacological Control of BAK, BAX, and Beyond.  |  Luna-Vargas, MPA. and Chipuk, JE. 2016. Trends Cell Biol. 26: 906-917. PMID: 27498846
2. BAK regulates catalase release from peroxisomes.  |  Fujiki, Y., et al. 2017. Mol Cell Oncol. 4: e1306610. PMID: 28616584
3. BCL-XL directly retrotranslocates the monomeric BAK.  |  Wang, B., et al. 2019. Cell Signal. 61: 1-9. PMID: 31059787
4. Structural basis of BAK activation in mitochondrial apoptosis initiation.  |  Singh, G., et al. 2022. Nat Commun. 13: 250. PMID: 35017502
5. Bak interacts with AKT and is involved in TNFα/CHX-induced apoptosis.  |  Song, J., et al. 2022. Mol Cell Biochem. 477: 939-949. PMID: 35094193
6. Mitochondrial E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins.  |  Huang, AS., et al. 2023. Cell Death Differ. 30: 632-646. PMID: 36171332
7. Sequence differences between BAX and BAK core domains manifest as differences in their interactions with lipids.  |  Miller, MS., et al. 2024. FEBS J. 291: 2335-2353. PMID: 38088212
8. A novel inhibitory BAK antibody enables assessment of non-activated BAK in cancer cells.  |  Subas Satish, HP., et al. 2024. Cell Death Differ. 31: 711-721. PMID: 38582955
9. Key residues in the VDAC2-BAK complex can be targeted to modulate apoptosis.  |  Yuan, Z., et al. 2024. PLoS Biol. 22: e3002617. PMID: 38696533
10. A lipid signature of BAK-driven apoptotic pore formation.  |  Uren, RT., et al. 2024. bioRxiv.. PMID: 39463966

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bak Antibody (G-10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Bak Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bak Antibody (G-10) Specifications

Alternate Product Name: Bak

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 41-60

Epitope Target: Bak

Epitope Species: mouse

Product Reactivity/Detection: Bak

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Bak Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Bak antibody is Bak Antibody (C-3): sc-518110.