Anticorpo p-Rsk (C-5) raccomandato per il rilevamento di Ser 380 phosphorylated Rsk di origine mouse, rat e human in WB, IP, IF e ELISA

Anticorpo p-Rsk (C-5): sc-377526

Anticorpo p-Rsk (C-5) valutazione 4.5 di 5 di 4.
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Schede tecniche

    • p-Rsk Antibody C-5 è un monoclonale di topo IgG2a κ, citato in 2 pubblicazioni, fornito in 200 µg/ml
    • specific for an epitope mapping to a short amino acid sequence containing phosphorylated Ser 380 of Rsk -1 of human origin
    • p-Rsk Antibody (C-5) é raccomandato per il rilevamento di Ser 380 phosphorylated Rsk di origine mouse, rat e human in WB, IP, IF e ELISA
    • p-Rsk Antibody (C-5) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di p-Rsk (C-5): sc-377526.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for p-Rsk Antibody (C-5) for WB applications. These reagents are now offered in bundles with p-Rsk Antibody (C-5) (see ordering information below).
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What application is the blocking peptide sc-377526 P appropriate for?

Richiesta da: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Risposta da: Technical Support
Data di pubblicazione: 2017-02-27
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Rated 5 di 5 di da Muy buen resultado para WB Muy buen resultado para WB. La banda es específica en extractos celulares totales de células AGS.
Data di pubblicazione: 2018-01-11
Rated 4 di 5 di da Works well for WB works well for western - samples were human cells in culture
Data di pubblicazione: 2017-11-21
Rated 4 di 5 di da Good antibody for Western blotting This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and was determined to be suitable for Western blotting. It provided medium detection of the Rsk target in immunoblots of lysates from HeLa cells that were exposed to anisomycin for 2 hours, but no other cross-reactivities were observed. There was no detection of the Rsk target in overnight serum-starved human A431 cells treated for 5 minutes with epidermal growth factor on Western blots, but again this antibody was very clean and no significant cross-reactivities on these blots with any off-target proteins.
Data di pubblicazione: 2016-12-08
Rated 5 di 5 di da Nice Western blot data of Rsk phosphorylation Nice Western blot data of Rsk phosphorylation in untreated, PMA treated and PMA and lambda protein phosphatase treated HeLa whole cell lysates. -SCBT QC
Data di pubblicazione: 2013-11-03
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