IRF3 Antibody (SL-14): sc-33642

IRF-3 Antibody (SL-14) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target the Interferon Regulatory Factor 3 (IRF-3) protein with exceptional precision. Anti-IRF-3 antibody (SL-14) is raised against a recombinant IRF-3 fusion protein corresponding to the human IRF-3 sequence, ensuring robust detection of IRF-3 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). IRF-3, also called IIAE7, is a pivotal transcription factor primarily located in the cytoplasm of cells under basal conditions, where IRF-3 remains inactive. Upon detection of viral infections or other pathogenic stimuli, IRF-3 undergoes phosphorylation, which triggers dimerization and translocation into the nucleus. Once in the nucleus, IRF-3 binds to specific DNA elements to activate the transcription of type I interferons, such as IFN-β, and other antiviral genes, orchestrating a critical innate immune response. The precise localization and activation of IRF-3 are essential for initiating effective antiviral defenses and regulating immune signaling pathways. IRF-3 monoclonal antibody (SL-14) enables researchers to accurately monitor the spatial and temporal dynamics of IRF-3 within cells, facilitating a deeper understanding of IRF-3′s role in immune regulation, pathogen response, and the molecular mechanisms underlying various diseases. Anti-IRF-3 antibody (SL-14) is an invaluable tool for studies aiming to elucidate the complex interactions and functions of IRF-3 in maintaining cellular homeostasis and combating infections.

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IRF3 Antibody (SL-14) References:

1. IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling.  |  Lazear, HM., et al. 2013. PLoS Pathog. 9: e1003118. PMID: 23300459
2. IRF-3, IRF-7, and IPS-1 promote host defense against acute human metapneumovirus infection in neonatal mice.  |  Spann, KM., et al. 2014. Am J Pathol. 184: 1795-806. PMID: 24726644
3. Cloning and characterizing of the murine IRF-3 gene promoter region.  |  Xu, HG., et al. 2016. Immunol Res. 64: 969-77. PMID: 26740329
4. IRF-3 gene polymorphisms are associated with the susceptibility to and the survival in chronic lymphocytic leukemia and could also serve as an auxiliary index.  |  Zhang, ZX., et al. 2017. Leuk Lymphoma. 58: 646-654. PMID: 27348780
5. Up-regulation of IRF-3 expression through GATA-1 acetylation by histone deacetylase inhibitor in lung adenocarcinoma A549 cells.  |  Wang, LL., et al. 2017. Oncotarget. 8: 75943-75951. PMID: 29100282
6. An IRF-3-, IRF-5-, and IRF-7-Independent Pathway of Dengue Viral Resistance Utilizes IRF-1 to Stimulate Type I and II Interferon Responses.  |  Carlin, AF., et al. 2017. Cell Rep. 21: 1600-1612. PMID: 29117564
7. The RNA helicase DDX3 promotes IFNB transcription via enhancing IRF-3/p300 holocomplex binding to the IFNB promoter.  |  Saikruang, W., et al. 2022. Sci Rep. 12: 3967. PMID: 35273248
8. Melatonin decreases IRF-3 protein expression in the gastrocnemius muscle, reduces IL-1β and LPS plasma concentrations, and improves the lipid profile in rats with apical periodontitis fed on a high-fat diet.  |  Dos Santos, RM., et al. 2023. Odontology. 111: 687-696. PMID: 36567367
9. Requirement of scavenger receptors for activation of the IRF-3/IFN-β/STAT-1 pathway in TLR4-mediated production of NO by LPS-activated macrophages.  |  de Queiroz, NMGP., et al. 2023. Nitric Oxide. 134-135: 61-71. PMID: 37059259
10. FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3.  |  Xu, Z., et al. 2023. Int J Mol Sci. 25: PMID: 38203523

The preferred IRF3 antibody is IRF3 Antibody (D-3): sc-376455.