caspase-3 Antibody (3CSP01): sc-65496

caspase-3 Antibody (3CSP01) is a mouse monoclonal IgG2a kappa light chain antibody engineered to recognize both active caspase-3 and its inactive precursor, procaspase-3, across mouse, rat, and human species. Caspase-3, also known as apopain, SCA-1, Yama, and CPP32, is a pivotal aspartate-specific cysteine protease within the ICE subfamily of caspases, primarily localized in the cytoplasm of cells. This strategic cytoplasmic location allows caspase-3 to swiftly execute apoptosis by cleaving key substrates, thereby facilitating the orderly dismantling of cellular components. During apoptosis, procaspase-3 is activated through proteolytic cleavage, forming the active enzyme composed of p17 and p11 subunits, which then orchestrates the apoptotic cascade by targeting proteins such as PARP and activating other caspases like caspase-6, -7, and -9. The ability of caspase-3 to reside in the cytoplasm is crucial for maintaining cellular homeostasis and eliminating damaged or unnecessary cells, which is essential in processes like development, immune response, and cancer prevention. caspase-3 monoclonal antibody (3CSP01) is indispensable for studying apoptotic mechanisms, offering reliable performance in applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). By targeting specific epitopes, anti-caspase-3 antibody (3CSP01) provides accurate detection and quantification, facilitating advanced research in cancer biology, neurodegeneration, and immune system regulation.

The preferred caspase-3 antibody is caspase-3 Antibody (E-8): sc-7272.