MEK-1 Antibody (H-8): sc-6250

MEK-1 Antibody (H-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects MEK-1 of mouse, rat, and human origin by WB, IP, IF, IHCP, and ELISA. MEK-1 Antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. MEK-1, also known as MAP2K1 or MKK1, plays a crucial role in the MAPK signaling pathway, which is essential for regulating various cellular processes such as proliferation, differentiation, and survival. MEK-1 is primarily located in the cytoplasm and is activated in response to growth factors and other extracellular signals, leading to translocation to the nucleus where MEK-1 phosphorylates and activates extracellular signal-regulated kinases (ERKs). This activation is vital for the transmission of signals from the cell surface to the nucleus, ultimately influencing gene expression and cellular responses. MEK-1 interaction with other proteins in the MAPK pathway, such as RAS and RAF, underscores MEK-1′s importance in cellular signaling and potential implications in cancer and other diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MEK-1 Antibody (H-8) References:

1. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
2. MEK 1/2 inhibitors in the treatment of hepatocellular carcinoma.  |  Facciorusso, A., et al. 2015. Expert Rev Gastroenterol Hepatol. 9: 993-1003. PMID: 25915713
3. The MEK 1/2 inhibitor PD98059 exhibits synergistic anti-endometrial cancer activity with paclitaxel in vitro and enhanced tissue distribution in vivo when formulated into PAMAM-coated PLGA-PEG nanoparticles.  |  Wiwatchaitawee, K., et al. 2022. Drug Deliv Transl Res. 12: 1684-1696. PMID: 34635984
4. MEK 1 inhibition and bleeding in hereditary haemorrhagic telangiectasia.  |  Shovlin, CL., et al. 2024. Br J Haematol. 204: 361-365. PMID: 37872650
5. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
6. Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
7. Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2.  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
8. Characterization of the structure and function of a novel MAP kinase kinase (MKK6).  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
9. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta).  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
10. Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase.  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
11. MKK7 is a stress-activated mitogen-activated protein kinase kinase functionally related to hemipterous.  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
12. Molecular cloning and characterization of human JNKK2, a novel Jun NH2-terminal kinase-specific kinase.  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK-1 Antibody (H-8) has 98 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK-1 Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK-1 Antibody (H-8) Specifications

Alternate Product Name: MAP2K1

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-393

Epitope Target: MEK-1

Epitope Species: human

Product Reactivity/Detection: MEK-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 98 citations in various scientific publications

Available Conjugates/Forms for MEK-1 Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MEK-1/2 antibody is MEK-1/2 Antibody (9G3): sc-81504.