RDH14 Activators
Chemical activators of RDH14 include a variety of molecules that interact with the enzyme to modulate its activity. Retinol itself can initiate a positive feedback loop by serving as a substrate for RDH14, resulting in the production of all-trans retinal. As the concentration of all-trans retinal increases, it further activates RDH14, enhancing the enzyme's ability to convert additional retinol into retinaldehyde. The cofactors NAD+ and NADP+ are essential for RDH14's enzymatic function, with NAD+ participating in the reduction of retinaldehyde to retinol and NADP+ involved in the oxidation of retinol to retinaldehyde. These cofactors are integral to the enzyme's activity, providing necessary hydride ions for the redox reactions that RDH14 catalyzes.
Additionally, the activation of RDH14 can be influenced by the composition of the cellular membrane, where the enzyme is located. Phospholipids such as phosphatidylethanolamine and lecithin can integrate into the membrane, potentially enhancing the fluidity and accessibility of lipid-soluble substrates to RDH14. Similarly, fatty acids like linoleic acid, arachidonic acid, oleic acid, and palmitic acid can modify the lipid bilayer's properties, facilitating RDH14's interaction with its retinoid substrates. Cholesterol, another key component of cell membranes, can also influence the membrane environment and, consequently, RDH14 activity. Finally, docosahexaenoic acid (DHA) is known for its role in altering membrane dynamics, which can support the proper functioning and activation of RDH14 by improving the enzyme's interactions with its substrates within the lipid-rich environment of the membrane. Each of these chemicals plays a role in modulating the physical and chemical conditions necessary for RDH14 to function optimally.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Vitamin A | 68-26-8 | sc-280187 sc-280187A | 1 g 10 g | $377.00 $2602.00 | ||
Retinol serves as a ligand for retinoid-binding proteins and is involved in the visual cycle. RDH14, as a retinol dehydrogenase, can be activated by the presence of its substrate, retinol, to catalyze the reduction of retinaldehyde in the metabolism of vitamin A. | ||||||
all-trans Retinal | 116-31-4 | sc-210778A sc-210778 | 250 mg 1 g | $126.00 $372.00 | 7 | |
All-trans retinal is a product of the reaction catalyzed by RDH14 and can activate RDH14 by substrate-induced enzyme activation, whereby the increased concentration of the product enhances the enzyme's activity in a positive feedback loop. | ||||||
NAD+, Free Acid | 53-84-9 | sc-208084B sc-208084 sc-208084A sc-208084C sc-208084D sc-208084E sc-208084F | 1 g 5 g 10 g 25 g 100 g 1 kg 5 kg | $56.00 $186.00 $296.00 $655.00 $2550.00 $3500.00 $10500.00 | 4 | |
NAD+ is a cofactor for RDH14 and its presence is essential for the enzymatic activity of RDH14. The binding of NAD+ to RDH14 activates the enzyme by facilitating the hydride transfer from NADH to retinal, the catalytic step in the reduction of retinal to retinol. | ||||||
β-Nicotinamide adenine dinucleotide phosphate | 53-59-8 | sc-215560 sc-215560A | 100 mg 250 mg | $114.00 $198.00 | ||
NADP+ is another cofactor of RDH14. The presence of NADP+ can activate RDH14 as it is involved in the oxidation-reduction reactions catalyzed by the enzyme, similar to NAD+, thus promoting the conversion of retinol to retinaldehyde. | ||||||
Linoleic Acid | 60-33-3 | sc-200788 sc-200788A sc-200788B sc-200788C | 100 mg 1 g 5 g 25 g | $33.00 $63.00 $163.00 $275.00 | 4 | |
Linoleic acid, as a component of the lipid bilayer, may influence membrane properties, potentially enhancing the interaction between RDH14 and its lipid-soluble substrates, thereby facilitating enzyme activation. | ||||||
Arachidonic Acid (20:4, n-6) | 506-32-1 | sc-200770 sc-200770A sc-200770B | 100 mg 1 g 25 g | $90.00 $235.00 $4243.00 | 9 | |
Arachidonic acid may alter the lipid composition of membranes, which could enhance the catalytic efficiency and activation of RDH14 by improving substrate accessibility or enzyme conformation. | ||||||
Cholesterol | 57-88-5 | sc-202539C sc-202539E sc-202539A sc-202539B sc-202539D sc-202539 | 5 g 5 kg 100 g 250 g 1 kg 25 g | $26.00 $2754.00 $126.00 $206.00 $572.00 $86.00 | 11 | |
Cholesterol is a major component of cell membranes and can affect membrane fluidity and structure, potentially promoting the activation of RDH14 by altering the membrane environment where RDH14 is active. | ||||||
Oleic Acid | 112-80-1 | sc-200797C sc-200797 sc-200797A sc-200797B | 1 g 10 g 100 g 250 g | $36.00 $102.00 $569.00 $1173.00 | 10 | |
Oleic acid may modify the lipid bilayer's properties, which could lead to an increase in RDH14 activity by enhancing the interaction between RDH14 and its substrates in the membrane. | ||||||
Docosa-4Z,7Z,10Z,13Z,16Z,19Z-hexaenoic Acid (22:6, n-3) | 6217-54-5 | sc-200768 sc-200768A sc-200768B sc-200768C sc-200768D | 100 mg 1 g 10 g 50 g 100 g | $92.00 $206.00 $1744.00 $7864.00 $16330.00 | 11 | |
Docosahexaenoic acid (DHA) can influence the physical properties of cell membranes, which might lead to the activation of RDH14 by improving the enzyme's interaction with its retinoid substrates within the lipid bilayer. | ||||||
Palmitic Acid | 57-10-3 | sc-203175 sc-203175A | 25 g 100 g | $112.00 $280.00 | 2 | |
Palmitic acid could affect the membrane structure and fluidity, possibly leading to the activation of RDH14 by facilitating the enzyme's interaction with its retinoid substrates or by affecting the enzyme's conformation for optimal activity. | ||||||