RBM28 Inhibitors

Chemical inhibitors of RBM28 can exert their inhibitory effects through various mechanisms that impact the protein's interaction with cellular components and its role in RNA processing. Phalloidin, for instance, binds actin filaments, leading to their stabilization. This stabilization can impede RBM28, which depends on dynamic actin structures for proper localization and function within the nucleus. Similarly, Latrunculin A disrupts microfilament organization by binding actin monomers, which can inhibit RBM28's interaction with actin, an interaction that might be necessary for its nuclear functions. Jasplakinolide also stabilizes actin filaments, and this action can inhibit RBM28 by preventing the actin remodeling that is required for RBM28's activity. Conversely, Cytochalasin D binds to the barbed ends of actin filaments and blocks their polymerization, which can inhibit RBM28's association with actin filaments that are required for its function.

Further inhibition of RBM28 can occur through the disruption of protein synthesis and RNA processing pathways. Withaferin A, known to bind annexin II, can inhibit RBM28 by disrupting the annexin II network, which is significant for mRNA processing and transport, functions involving RBM28. Rocaglamide, an inhibitor of translation initiation, can inhibit RBM28 by preventing the synthesis of interacting or supporting proteins necessary for RBM28's function. Silvestrol, a disruptor of eIF4A RNA helicase activity, can inhibit RBM28 by interfering with RNA processing and translation initiation. Pateamine A, by binding to eIF4A, can inhibit RBM28 by affecting the translation of crucial proteins for RBM28's operation. Harringtonine and its derivative Homoharringtonine target the protein synthesis machinery; Harringtonine inhibits initiation and Homoharringtonine inhibits elongation, both of which can inhibit RBM28 by reducing the synthesis of essential proteins for RBM28's RNA processing role. Emetine and Cycloheximide, both protein synthesis inhibitors, can inhibit RBM28 by impeding the production of proteins necessary for RBM28's mRNA processing functions within the nucleus, thus inhibiting the protein's overall activity.