RBFA Inhibitors
Chemical inhibitors of RBFA can act through various mechanisms to hamper its function in the ribosome biogenesis pathway. Staurosporine, a kinase inhibitor, can disrupt the phosphorylation state of RBFA, which is essential for its activity and interaction with other ribosomal proteins. This inhibition can lead to a functional loss of RBFA. Similarly, Rapamycin targets the mTOR pathway, which is crucial for cell growth and protein synthesis. By inhibiting mTOR, Rapamycin can reduce ribosomal protein translation, effectively limiting the incorporation of RBFA into forming ribosomes. Cycloheximide and Anisomycin interfere with the translocation and peptidyl transferase activity in protein synthesis, respectively, leading to a reduction in overall protein synthesis and indirectly reducing the functional presence of RBFA in ribosome assembly.
Furthermore, Chloramphenicol can hinder the bacterial ribosome, and due to structural similarities, this can extrapolate to an indirect inhibition of RBFA's eukaryotic ribosomal function. Tunicamycin's inhibition of N-linked glycosylation can lead to endoplasmic reticulum stress and a subsequent decrease in overall protein folding capacity, affecting RBFA's role in ribosome assembly. Puromycin and Harringtonine disrupt translation by causing premature chain termination and inhibiting the initial elongation step, respectively, which can limit the function of RBFA in ribosome assembly. Emetine targets the eukaryotic ribosomal 40S subunit, inhibiting translocation of peptidyl-tRNA, which is essential for ribosome assembly involving RBFA. Ricin inactivates ribosomes by depurinating a specific adenine residue in rRNA, a key process in which RBFA is involved. α-Amanitin inhibits RNA polymerase II, reducing the synthesis of mRNAs coding for ribosomal proteins and assembly factors, thereby indirectly inhibiting RBFA. Lastly, Pactamycin's binding to the 30S ribosomal subunit can also indirectly inhibit RBFA by interfering with its role in the assembly and function of ribosomes.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $150.00 $388.00 | 113 | |
Staurosporine is a potent inhibitor of protein kinases. RBFA, being involved in ribosome biogenesis, relies on phosphorylation for its activity and interaction with other proteins. Inhibition of these kinases by Staurosporine can inhibit RBFA by preventing its necessary phosphorylation, leading to a loss of its function. | ||||||
Rapamycin | 53123-88-9 | sc-3504 sc-3504A sc-3504B | 1 mg 5 mg 25 mg | $62.00 $155.00 $320.00 | 233 | |
Rapamycin specifically inhibits mTOR, a kinase involved in cell growth and protein synthesis. As RBFA is part of the protein synthesis machinery, inhibition of mTOR by Rapamycin can lead to reduced ribosomal protein translation, indirectly inhibiting RBFA function by limiting its incorporation into ribosomes. | ||||||
Cycloheximide | 66-81-9 | sc-3508B sc-3508 sc-3508A | 100 mg 1 g 5 g | $40.00 $82.00 $256.00 | 127 | |
Cycloheximide inhibits eukaryotic protein biosynthesis by interfering with the translocation step in protein synthesis, thus inhibiting the elongation process. This inhibition can decrease the overall protein synthesis rate, including that of the ribosomal proteins and assembly factors, thereby inhibiting RBFA function. | ||||||
Chloramphenicol | 56-75-7 | sc-3594 | 25 g | $53.00 | 10 | |
Chloramphenicol binds to the bacterial ribosome and inhibits protein synthesis. While RBFA is eukaryotic, the similarity in ribosomal structure means that inhibition of ribosomes by Chloramphenicol can indirectly inhibit RBFA by disrupting its ribosomal association and function. | ||||||
Tunicamycin | 11089-65-9 | sc-3506A sc-3506 | 5 mg 10 mg | $169.00 $299.00 | 66 | |
Tunicamycin inhibits N-linked glycosylation, which can affect the folding and stability of glycoproteins. While RBFA is not a glycoprotein, the inhibition of glycosylation can lead to ER stress and a general inhibition of protein folding, indirectly affecting RBFA's ribosomal assembly activity. | ||||||
Puromycin | 53-79-2 | sc-205821 sc-205821A | 10 mg 25 mg | $163.00 $316.00 | 436 | |
Puromycin causes premature chain termination during translation due to its structural similarity to aminoacyl-tRNAs. This can lead to the inhibition of protein synthesis, indirectly affecting RBFA's role in ribosome biogenesis. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin interferes with protein synthesis by inhibiting peptidyl transferase activity on ribosomes. This inhibition can indirectly reduce RBFA's role in ribosome biogenesis by preventing proper ribosome function. | ||||||
Emetine | 483-18-1 | sc-470668 sc-470668A sc-470668B sc-470668C | 1 mg 10 mg 50 mg 100 mg | $352.00 $566.00 $1331.00 $2453.00 | ||
Emetine inhibits protein synthesis by binding to the eukaryotic ribosomal 40S subunit, causing an inhibition of translocation of peptidyl-tRNA from the A to the P site. This can indirectly inhibit RBFA's function in ribosome assembly. | ||||||
α-Amanitin | 23109-05-9 | sc-202440 sc-202440A | 1 mg 5 mg | $260.00 $1029.00 | 26 | |
α-Amanitin inhibits RNA polymerase II, which is responsible for transcribing mRNA. By inhibiting mRNA synthesis, α-Amanitin indirectly inhibits RBFA's role in ribosome biogenesis, as fewer ribosomal proteins and assembly factors will be produced. | ||||||
Harringtonin | 26833-85-2 | sc-204771 sc-204771A sc-204771B sc-204771C sc-204771D | 5 mg 10 mg 25 mg 50 mg 100 mg | $195.00 $350.00 $475.00 $600.00 $899.00 | 30 | |
Harringtonine inhibits protein synthesis by preventing the initial elongation step of translation. This can indirectly inhibit RBFA function by disrupting ribosome assembly, as RBFA is involved in the assembly of ribosomal subunits. | ||||||