PuromycinCAS: 53-79-2
MF: C22H29N7O5
MW: 471.51
An aminonuclease antibiotic produced by the soil actinomycete Streptomyces alboniger; which induces apoptosis.
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Puromycin (CAS 53-79-2)

Puromycin | CAS 53-79-2 is rated 5.0 out of 5 by 1.
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See product citations (144)
Synonym: Stylomycin; Achromycin; Stillomycin; Bacterenomycin
Application: An aminonuclease antibiotic produced by the soil actinomycete Streptomyces alboniger; which induces apoptosis
CAS Number: 53-79-2
Purity: >98%
Molecular Weight: 471.51
Molecular Formula: C22H29N7O5
* Refer to Certificate of Analysis for lot specific data (including water content).
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Puromycin is an aminonuclease antibiotic produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Toxic to both eukaryotic and prokaryotic cells. Believed to act as an acyl-tRNA analogue causing premature chain termination through covalent peptidyl-puromycin condensation; inducing apoptosis in a variety of cells. This induction of apoptosis was found to parallel apoptosis caused by deprivation of neuronal growth factor (NGF) in developing chick sympathetic neurons; however, this apoptotic activity can be inhibited by several transcriptional inhibitors such as: actinomycin D (sc-200906) and α-amanitin (sc-202440) which has lead researchers to hypothesize that apoptosis is triggered through the transcription of particular genes. Shown to arrest cells in G2/M phase. Puromycin is an inhibitor of CD13 and DPP7. Puromycin is also a substrate of Mdr-1.


References

1. Brunetti, M., et al. 1995. Blood. 86: 4199-4205. PMID: 7492778
2. Constam, D.B., et al. 1995. J. Biol. Chem. 270: 26931-26939. PMID: 7592939
3. Sugita, M., et al. 1995. Zool. Sci. 12: 419-425. PMID: 8528014
4. Tabuchi, I. 2003. Biochem. Biophys. Res. Commun. 305: 1-5. PMID: 12732187

Usage :
This product has not been evaluated for use in cell culture applications. For the selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles, we recommend the following: Puromycin dihydrochloride (sc-108071)
Appearance :
Powder
Physical State :
Solid
Derived From :
Streptomyces alboniger
Solubility :
Soluble in ethanol, methanol, DMF or DMSO. Moderate water solubility.
Storage :
Store at -20° C
Refractive Index :
n20D 1.70 (Predicted)
pK Values :
pKa: 13 (Predicted), pKb: 7.44 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
RTECS :
AU7350000
PubChem CID :
439530
Merck Index :
14: 7943
MDL Number :
MFCD00012691
Beilstein Registry :
3853613
SMILES :
CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O

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Puromycin  Product Citations

See how others have used Puromycin. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 144 total

PMID: # 30873505  Khoury, J.|Selezneva, I.|Pestov, S.|Tarassov, V.|Ermakov, A.|Mikheev, A.|Lazov, M.|Kirkpatrick, SR.|Shashkov, D.|Smolkov, A.| et al. 2019. Bioact Mater. 4: 132-141.

PMID: # 30753671  Noh, JH.|Kim, KM.|Pandey, PR.|Hooten, NN.|Munk, R.|Kundu, G.|De, S.|Martindale, JL.|Yang, X.|Evans, MK.|Abdelmohsen, K.|Gorospe, M.| et al. 2019. Nucleic Acids Res.

PMID: # 31140665  DeOre, BJ.|Galie, PA.|Sehgal, CM.| et al. 2019. Microcirculation. e12576.

PMID: # 31239444  Yang, S.|Wei, J.|Cui, YH.|Park, G.|Shah, P.|Deng, Y.|Aplin, AE.|Lu, Z.|Hwang, S.|He, C.|He, YY.| et al. 2019. Nat Commun. 10: 2782.

PMID: # 31043744  Wang, W. et al. 2019. Nature. 569: 270-274.

PMID: # 30484958  Roversi, FM.|Cury, NM.|Lopes, MR.|Ferro, KP.|Machado-Neto, JA.|Alvarez, MC.|Dos Santos, GP.|Giardini Rosa, R.|Longhini, AL.|Duarte, ADSS.|Pericole, FV.|Favaro, P.|Yunes, JA.|Saad, STO.| et al. 2019. J. Cell. Mol. Med. 23: 1562-1571.

PMID: # 31189704  Liu, X.|Matrenec, R.|Gack, MU.|He, B.| et al. 2019. J. Virol. 93:

PMID: # 31431634  2019. Sci Rep. 9: 12135.

PMID: # 30970625  Kim, T.|Ahmad, K.|Shaikh, S.|Jan, AT.|Seo, MG.|Lee, EJ.|Choi, I.| et al. 2019. Cells. 8:

PMID: # 30859713  Ishida, N. et al. 2019. Am. J. Transplant.

Citations 1 to 10 of 144 total

What else is this soluble in?

Asked by: chemicalsmg
Thank you for your question. The chemical, sc-205821, is soluble in ethanol, methanol, DMF and DMSO. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-11
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Rated 5 out of 5 by from Fritsch Fritsch, et. al. (PubMed ID 24980434) utilized sc-205821, puromycin, as a selection agent to obtain stable shRNA expressing clones. -SCBT Publication Review
Date published: 2015-04-14
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