Popular Stains, Dyes and Fluorescent Probes

Acid fuchsin is a versatile synthetic dye characterized by its striking color and ability to bind to various biological tissues. Its unique molecular structure allows for strong electrostatic interactions with proteins and polysaccharides, enhancing its staining capabilities. This dye exhibits distinct fluorescence properties, making it useful for visualizing cellular components under specific light conditions. Its stability and solubility in aqueous solutions further contribute to its effectiveness in diverse staining protocols.

Fluorescein isothiocyanate isomer I is a vibrant fluorescent probe known for its exceptional reactivity with amines and thiols, facilitating the formation of stable conjugates. Its unique isothiocyanate group enables selective labeling of biomolecules, enhancing visualization in microscopy. The compound exhibits high quantum yield and photostability, making it ideal for long-term imaging studies. Additionally, its solubility in polar solvents allows for versatile applications in various experimental setups.

Eosin Y sodium salt is a synthetic dye renowned for its vibrant fluorescence and affinity for proteins, nucleic acids, and lipids. Its anionic nature allows for strong ionic interactions with positively charged biomolecules, enhancing staining efficiency. The compound exhibits unique photophysical properties, including a high quantum yield and photostability, making it ideal for various imaging techniques. Its rapid diffusion in biological media facilitates real-time observation of cellular processes.

Rhodamine B isothiocyanate is a vibrant fluorescent dye characterized by its strong affinity for amino groups, allowing for selective labeling of proteins and nucleic acids. Its unique isothiocyanate functional group facilitates covalent bonding, enhancing stability and signal intensity in fluorescence microscopy. The compound exhibits high photostability and a broad excitation spectrum, making it ideal for multiplexing applications. Its intense pink fluorescence provides clear contrast, aiding in the visualization of cellular structures.

1-Pyrenesulfonic acid sodium salt is a prominent fluorescent probe characterized by its strong photostability and high quantum yield. Its pyrene moiety exhibits distinct π-π stacking interactions, enhancing its ability to bind to various substrates. This compound's unique sulfonic acid group increases water solubility, facilitating its use in aqueous environments. Additionally, its sensitivity to environmental changes allows for real-time monitoring of molecular dynamics, making it an effective tool in analytical chemistry.

Sulforhodamine 101 is a vibrant fluorescent dye known for its exceptional photostability and high quantum yield, making it ideal for various imaging applications. Its unique structure allows for strong interactions with proteins and nucleic acids, enhancing its utility as a biological probe. The dye exhibits distinct absorption and emission characteristics, enabling precise tracking in complex biological systems. Additionally, its anionic nature promotes solubility in aqueous environments, facilitating versatile experimental designs.

Lucifer Yellow CH dipotassium salt is a vibrant fluorescent dye characterized by its exceptional ability to permeate cell membranes and label cellular structures. Its unique sulfonate groups facilitate strong ionic interactions with proteins, enhancing its visibility in microscopy. The dye exhibits a distinct excitation and emission spectrum, making it ideal for multi-labeling applications. Its photostability ensures consistent performance during imaging, allowing for prolonged observation of dynamic biological processes.

Fluorescein 5-Maleimide is a highly effective fluorescent probe known for its selective reactivity with thiol groups, enabling the labeling of proteins and biomolecules. Its unique maleimide functionality allows for stable covalent bonding, resulting in bright fluorescence that enhances visibility in various assays. The compound exhibits rapid reaction kinetics, making it suitable for real-time monitoring of molecular interactions. Additionally, its water solubility and pH stability contribute to its versatility in diverse experimental conditions.

Dihydrorhodamine 123 is a versatile fluorescent probe that exhibits remarkable sensitivity to reactive species, particularly in oxidative environments. Upon oxidation, it transforms into a highly fluorescent derivative, enabling real-time monitoring of cellular dynamics. Its unique ability to permeate cell membranes and interact with various biomolecules enhances its utility in visualizing cellular processes. The probe's rapid response to changes in redox conditions makes it invaluable for studying cellular metabolism and stress responses.

Di-8-ANEPPS is a fluorescent probe characterized by its unique ability to selectively bind to lipid membranes, making it an effective tool for studying membrane dynamics. Its distinct molecular structure allows for enhanced sensitivity to local electric fields, resulting in a notable shift in fluorescence upon membrane polarization. This property enables real-time monitoring of membrane potential changes, providing insights into cellular activity and signaling pathways. The compound's high quantum yield and stability further enhance its utility in advanced imaging applications.