Molecular structure of Fluorescein 5-Maleimide, CAS Number: 75350-46-8
Fluorescein 5-Maleimide (CAS 75350-46-8)
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Alternate Names:
5-MF
Application:
Fluorescein 5-Maleimide is a protein fluorescent labeling reagent
CAS Number:
75350-46-8
Molecular Weight:
427.36
Molecular Formula:
C24H13NO7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Fluorescein 5-Maleimide is a fluorescent dye, labels and tracks proteins and other cellular components in biology. It covalently binds to thiol groups on proteins, allowing for visualization and quantification of specific molecules within cells. This labeling technique is used for studying protein localization, trafficking, and interactions within cellular pathways. The mechanism of action involves the maleimide group reacting with thiol groups on cysteine residues of proteins, forming a stable thioether bond. This allows for the visualization of the labeled proteins under a fluorescence microscope, providing insights into their cellular functions and dynamics.
Fluorescein 5-Maleimide (CAS 75350-46-8) References
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- Design of a structure-based fluorescent biosensor from bioengineered arginine deiminase for rapid determination of L-arginine. | Tam, SY., et al. 2020. Int J Biol Macromol. 165: 472-482. PMID: 32971169
- A method of screening for mutant proteins containing cysteine residues using fluorescein-5-maleimide. | McLachlin, DT. and Dunn, SD. 1996. Protein Expr Purif. 7: 275-80. PMID: 8861241
- A method for determining transmembrane protein structure. | Jones, PC., et al. 1996. Mol Membr Biol. 13: 53-60. PMID: 9147663
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fluorescein 5-Maleimide, 25 mg | sc-215041 | 25 mg | $267.00 |