KIAA0895L_4931428F04Rik Activators
Chemical activators of microtubule associated tyrosine carboxypeptidase 1 engage in a variety of interactions with microtubules to modulate the protein's activity. GTPγS acts as a non-hydrolyzable analog of GTP, directly stimulating G-proteins which in turn can promote the assembly and stabilization of microtubules, creating an environment conducive for the activation of microtubule associated tyrosine carboxypeptidase 1. Similarly, stabilizing agents like Paclitaxel and Epothilone B, prevent microtubule disassembly, ensuring continuous access for the carboxypeptidase to its tubulin substrates. Contrarily, Colchicine and Vinblastine function through disruption of microtubule assembly, yet still manage to activate the carboxypeptidase by possibly enhancing substrate accessibility through induced depolymerization or alteration of the microtubule network.
On another front, chemical inhibitors of protein phosphatases, such as Okadaic Acid and Calyculin A, lead to an increase in the phosphorylation state of proteins associated with microtubules, which can result in enhanced activity of microtubule associated tyrosine carboxypeptidase 1. Forskolin elevates cAMP levels that activate PKA, which may then phosphorylate microtubule-associated proteins, thereby influencing the activity of microtubule associated tyrosine carboxypeptidase 1. Nocodazole, by destabilizing microtubules, and S-Trityl-L-cysteine, through inhibition of microtubule polymerization, both can induce changes in microtubule dynamics that may lead to the activation of microtubule associated tyrosine carboxypeptidase 1. Anisomycin engages through activation of stress-activated protein kinases, which subsequently phosphorylate microtubule-associated proteins and can thus modulate the activity of microtubule associated tyrosine carboxypeptidase 1. Collectively, these chemicals, through distinct pathways involving microtubule dynamics and protein phosphorylation, can orchestrate the activation of microtubule associated tyrosine carboxypeptidase 1.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Guanosine 5′-O-(3-thiotriphosphate) tetralithium salt | 94825-44-2 | sc-202639 | 10 mg | $465.00 | ||
GTPγS is a non-hydrolyzable analog of GTP that activates G-proteins, which in turn can activate microtubule associated tyrosine carboxypeptidase 1 by promoting microtubule assembly and stability, thereby enhancing the protein's ability to regulate microtubule dynamics. | ||||||
Taxol | 33069-62-4 | sc-201439D sc-201439 sc-201439A sc-201439E sc-201439B sc-201439C | 1 mg 5 mg 25 mg 100 mg 250 mg 1 g | $41.00 $74.00 $221.00 $247.00 $738.00 $1220.00 | 39 | |
Paclitaxel stabilizes microtubules and can enhance the activation of microtubule associated tyrosine carboxypeptidase 1 by preventing microtubule disassembly, which is necessary for the protein's carboxypeptidase activity on tubulin. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases PP1 and PP2A; by inhibiting these phosphatases, it leads to increased phosphorylation of proteins involved in microtubule dynamics, thereby potentially enhancing the activity of microtubule associated tyrosine carboxypeptidase 1. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A, like Okadaic Acid, is also an inhibitor of PP1 and PP2A, which can result in the hyperphosphorylation of microtubule-associated proteins, potentially increasing the activity of microtubule associated tyrosine carboxypeptidase 1 through enhanced substrate availability. | ||||||
Colchicine | 64-86-8 | sc-203005 sc-203005A sc-203005B sc-203005C sc-203005D sc-203005E | 1 g 5 g 50 g 100 g 500 g 1 kg | $100.00 $321.00 $2289.00 $4484.00 $18207.00 $34749.00 | 3 | |
Colchicine binds to tubulin and inhibits its polymerization; low concentrations of colchicine can activate microtubule associated tyrosine carboxypeptidase 1 by inducing microtubule depolymerization, which may expose sites for enzymatic activity. | ||||||
Vinblastine | 865-21-4 | sc-491749 sc-491749A sc-491749B sc-491749C sc-491749D | 10 mg 50 mg 100 mg 500 mg 1 g | $102.00 $235.00 $459.00 $1749.00 $2958.00 | 4 | |
Vinblastine disrupts microtubule assembly and can lead to activation of microtubule associated tyrosine carboxypeptidase 1 by altering the microtubule network, potentially increasing the accessibility of carboxypeptidase substrates. | ||||||
Nocodazole | 31430-18-9 | sc-3518B sc-3518 sc-3518C sc-3518A | 5 mg 10 mg 25 mg 50 mg | $59.00 $85.00 $143.00 $247.00 | 38 | |
Nocodazole is a microtubule-depolymerizing agent that can activate microtubule associated tyrosine carboxypeptidase 1 by destabilizing microtubules, which may enhance the protein's access to tubulin substrates. | ||||||
Epothilone B, Synthetic | 152044-54-7 | sc-203944 | 2 mg | $176.00 | ||
Epothilone B is a microtubule stabilizing agent similar to paclitaxel and can activate microtubule associated tyrosine carboxypeptidase 1 by enhancing microtubule stability and increasing the protein's enzymatic activity on tubulin. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin activates stress-activated protein kinases which can phosphorylate microtubule-associated proteins. This phosphorylation can lead to the activation of microtubule associated tyrosine carboxypeptidase 1 by altering the microtubule network. | ||||||
S-Trityl-L-cysteine | 2799-07-7 | sc-202799 sc-202799A | 1 g 5 g | $32.00 $66.00 | 6 | |
S-Trityl-L-cysteine is a microtubule polymerization inhibitor that can paradoxically activate microtubule associated tyrosine carboxypeptidase 1 by causing alterations in microtubule dynamics, potentially increasing the accessibility and quantity of the protein's substrates. | ||||||