JMJD2B Activators
JMJD2B Activators are compounds that directly or indirectly enhance the enzymatic activity of JMJD2B, a histone demethylase. The activity of JMJD2B is dependent on its cofactors and substrates, which include 2-oxoglutarate, iron (II), and oxygen. 2-oxoglutarate is a substrate that binds to JMJD2B and is directly involved in the demethylation process, enhancing the enzyme's function. Ascorbic acid, also known as vitamin C, acts as a cofactor for enzymes that hydroxylate JMJD2B, leading to increased stability and enzymatic activity of JMJD2B.
Iron (II) sulfate supplies the Fe(II) that serves as an essential cofactor in the histone demethylation process catalyzed by JMJD2B. The presence of Fe(II) is crucial for JMJD2B's activity, and compounds like NADPH and TCEP help maintain the Fe(II) in its active reduced state. α-Ketoglutarate and NOG (as a competitive analog) are also involved in the catalytic process, with NOG being used experimentally to modulate the enzyme's activity for research purposes.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
L-Ascorbic acid, free acid | 50-81-7 | sc-202686 | 100 g | $46.00 | 5 | |
Ascorbic acid acts as a cofactor for Fe(II) prolyl hydroxylase, which is involved in the hydroxylation of the JMJD2B protein, leading to its stabilization and enhanced functional activity. | ||||||
Ferrous Sulfate (Iron II Sulfate) Heptahydrate | 7782-63-0 | sc-211505 sc-211505A | 250 g 500 g | $73.00 $109.00 | ||
Iron (II) sulfate provides Fe(II), an essential cofactor for JMJD2B, and its presence is required for the catalytic demethylation of histones, thus directly enhancing JMJD2B activity. | ||||||
β-Nicotinamide adenine dinucleotide phosphate | 53-59-8 | sc-215560 sc-215560A | 100 mg 250 mg | $182.00 $319.00 | ||
NADPH maintains the Fe(II) cofactor of JMJD2B in its reduced state, which is necessary for JMJD2B catalytic activity, thereby enhancing its function. | ||||||
Pyruvic acid | 127-17-3 | sc-208191 sc-208191A | 25 g 100 g | $41.00 $96.00 | ||
Pyruvate can supply cellular energy in the form of ATP, which is required for many cellular processes that indirectly enhance the activity of JMJD2B by maintaining cellular metabolism. | ||||||
Sodium Chloride | 7647-14-5 | sc-203274 sc-203274A sc-203274B sc-203274C | 500 g 2 kg 5 kg 10 kg | $19.00 $30.00 $60.00 $110.00 | 15 | |
Sodium chloride at physiological concentrations provides an optimal ionic environment for JMJD2B activity by maintaining protein stability and structure, thereby enhancing its function. | ||||||