I-SceI Inhibitors

Chemical Class Description: The class of I-SceI inhibitors consists of compounds that indirectly modulate the activity of I-SceI by affecting the DNA substrate with which it interacts. These compounds, while not directly interacting with I-SceI, influence DNA structure, replication, and repair mechanisms, crucial for I-SceI's role in DNA cleavage. DNA intercalating agents like Ethidium Bromide, Doxorubicin, Daunorubicin, Actinomycin D, and Chloroquine can alter the DNA conformation, thereby potentially hindering the ability of I-SceI to recognize and cleave its specific DNA site. DNA alkylating agents such as Mitomycin C and Cisplatin form adducts with DNA, which might interfere with the DNA recognition and cleavage by I-SceI.

Topoisomerase inhibitors like Camptothecin and Etoposide affect the topology of DNA, which could influence I-SceI activity. Aphidicolin, by inhibiting DNA polymerase, impacts DNA replication, potentially affecting the structure and availability of DNA for I-SceI action. Hydroxyurea and Bleomycin, through their effects on ribonucleotide reductase inhibition and DNA strand breakage, respectively, represent additional mechanisms that could indirectly impact the function of I-SceI.