hMLH3 Activators
hMLH3 Activators are a collection of chemical compounds that directly or indirectly promote the functional activity of hMLH3, which plays a critical role in the DNA mismatch repair (MMR) pathway. ATP is a direct activator, increasing hMLH3's ATPase activity needed for DNA binding and repair function. Similarly, zinc acetate and magnesium chloride act as stabilizing agents for hMLH3's structure and endonuclease activity, respectively, promoting its role in MMR. Calcium chloride facilitates the interaction of hMLH3 with other MMR proteins, enhancing the protein complex's ability to correct DNA mismatches. S-Adenosylmethionine and NAD+ serve as indirect activators by contributing to the methylation and sirtuin-mediated deacetylation processes, respectively, which enhance the recruitment and DNA binding affinity of hMLH3, thereby potentiating its repair function. Reduced glutathione maintains hMLH3 in a reduced and active state, while β-Nicotinamide mononucleotide (NMN) supports the biosynthesis of NAD+, further promoting sirtuin activity and hMLH3 functionality.
Chemical compounds like oxaliplatin and methotrexate indirectly enhance hMLH3 activity by increasing the cellular demand for DNA repair, thereby stimulating the MMR pathway in which hMLH3 is essential. Trichostatin A, by promoting histone acetylation, indirectly promotes hMLH3 activity by facilitating chromatin decondensation, enhancing the accessibility of the mismatch repair machinery to DNA errors. Lastly, 6-Thioguanine incorporation into DNA results in mismatches that are recognized and repaired by hMLH3, thereby enhancing its functional demand. Collectively, these hMLH3 Activators leverage various biochemical mechanisms to increase the activity of hMLH3, ensuring genomic stability through the proficient correction of DNA mismatches without the need for upregulating its expression or direct activation of the protein.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
ADP | 58-64-0 | sc-507362 | 5 g | $53.00 | ||
ATP directly enhances the ATPase activity of hMLH3, as the protein utilizes ATP binding and hydrolysis to exert its mismatch repair function. Increased ATP concentrations can promote the binding of hMLH3 to DNA mismatches, which is a crucial step in the repair process. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $47.00 | ||
Zinc acetate stabilizes the structure of hMLH3 by binding to its zinc finger domain. This interaction is crucial for the protein's ability to interact with other mismatch repair proteins, enhancing its functional activity in the repair pathway. | ||||||
Magnesium chloride | 7786-30-3 | sc-255260C sc-255260B sc-255260 sc-255260A | 10 g 25 g 100 g 500 g | $27.00 $34.00 $47.00 $123.00 | 2 | |
Magnesium ions are essential cofactors for hMLH3's endonuclease activity. By increasing the availability of magnesium, the chemical enhances hMLH3's ability to introduce nicks in the DNA during the mismatch repair process. | ||||||
Calcium chloride anhydrous | 10043-52-4 | sc-207392 sc-207392A | 100 g 500 g | $65.00 $262.00 | 1 | |
Calcium ions support the protein-protein interactions involving hMLH3 within the mismatch repair pathway. Adequate calcium levels can enhance the formation of the hMLH3 complex with other repair proteins, facilitating the repair of DNA mismatches. | ||||||
Ademetionine | 29908-03-0 | sc-278677 sc-278677A | 100 mg 1 g | $180.00 $655.00 | 2 | |
As a methyl donor, S-Adenosylmethionine indirectly enhances the activity of hMLH3 by donating a methyl group during the DNA methylation process. This modification can facilitate the recruitment of mismatch repair proteins including hMLH3 to sites of DNA damage. | ||||||
NAD+, Free Acid | 53-84-9 | sc-208084B sc-208084 sc-208084A sc-208084C sc-208084D sc-208084E sc-208084F | 1 g 5 g 10 g 25 g 100 g 1 kg 5 kg | $56.00 $186.00 $296.00 $655.00 $2550.00 $3500.00 $10500.00 | 4 | |
NAD+ indirectly enhances hMLH3 function by serving as a substrate for sirtuins, which modify the post-translational state of hMLH3. Sirtuin-mediated deacetylation can increase the DNA binding affinity of hMLH3, thus enhancing its mismatch repair activity. | ||||||
Glutathione, reduced | 70-18-8 | sc-29094 sc-29094A | 10 g 1 kg | $76.00 $2050.00 | 8 | |
Glutathione reduces oxidative stress within cells, thereby preserving the integrity of proteins including hMLH3. Reduced glutathione maintains cysteine residues in a reduced state, allowing hMLH3 to maintain its proper structure and function in DNA repair. | ||||||
β-Nicotinamide mononucleotide | 1094-61-7 | sc-212376 sc-212376A sc-212376B sc-212376C sc-212376D | 25 mg 100 mg 1 g 2 g 5 g | $92.00 $269.00 $337.00 $510.00 $969.00 | 4 | |
NMN enhances hMLH3 activity by serving as a precursor for NAD+ synthesis, thus contributing to the same sirtuin-mediated post-translational modifications of hMLH3 that enhance its activity in DNA mismatch repair as described for NAD+. | ||||||
Oxaliplatin | 61825-94-3 | sc-202270 sc-202270A | 5 mg 25 mg | $110.00 $386.00 | 8 | |
Oxaliplatin induces DNA damage that requires the mismatch repair system for correction. The presence of oxaliplatin increases the demand for hMLH3 activity, thereby indirectly enhancing its functional role in DNA repair. | ||||||
Methotrexate | 59-05-2 | sc-3507 sc-3507A | 100 mg 500 mg | $92.00 $209.00 | 33 | |
Methotrexate indirectly enhances hMLH3 activity by causing DNA damage through inhibition of dihydrofolate reductase, increasing the recruitment and action of hMLH3 in the mismatch repair process. | ||||||