GnT-I Activators
GnT-I glycosylates target proteins within the Golgi apparatus, relying on adequate substrate levels and proper trafficking. Key regulators include UDP-GlcNAc and related sugar nucleotide donors, which compounds like glucosamine, STF-31, and PugNac boost through hexosamine and O-GlcNAc pathway effects. N-acetylmannosamine complements these inputs. Regarding intracellular transport, certain trafficking modulators such as low dose Brefeldin A, and Retro-2 for optimal cargo modification. PIP3 and Golgicide A analogs might refine trafficking. Unexpectedly, depleting siRNA could enhance activity through mRNA splicing rather than knockdown. Collectively, these agents act on GnT-I in its native Golgi environment or influence associated substrates and transport pathways critical to its remodeling functions.
Determining how individual compounds or synergistic combinations modulate GnT-I capacity and trafficking presents opportunities. Fine-tuning localization amid the dynamic Golgi could impact glycan outcomes on select cargoes.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
N-Acetyl-D-glucosamine | 7512-17-6 | sc-286377 sc-286377B sc-286377A | 50 g 100 g 250 g | $94.00 $162.00 $306.00 | 1 | |
UDP-GlcNAc donates the GlcNAc moiety for GnT-I-mediated addition to glycoprotein acceptors. Elevated UDP-GlcNAc stimulates GnT-I activity. | ||||||
D-Galactose | 59-23-4 | sc-202564 | 100 g | $288.00 | 4 | |
Glucosamine feeds into the hexosamine biosynthesis pathway to generate increased UDP-GlcNAc, the donor substrate for GnT-I. | ||||||
STF 31 | 724741-75-7 | sc-364692 | 10 mg | $187.00 | 3 | |
STF-31 inhibits GFAT to boost the UDP-GlcNAc pool available for GnT-I-catalyzed modification of glycoproteins. | ||||||
(Z)-Pugnac | 132489-69-1 | sc-204415A sc-204415 | 5 mg 10 mg | $224.00 $380.00 | 3 | |
The O-GlcNAcase inhibitor PugNac enhances UDP-GlcNAc levels by preventing its degradation, increasing GnT-I activity. | ||||||
Golgicide A | 1005036-73-6 | sc-215103 sc-215103A | 5 mg 25 mg | $191.00 $683.00 | 11 | |
At low doses, this compound increases GnT-I concentration and activity in the cis-Golgi through alterations in membrane traffic. | ||||||