Em 450-495 nm Blue

DAPI is a fluorescent dye that exhibits strong emission in the 450-495 nm range, primarily due to its ability to intercalate into DNA. This intercalation leads to enhanced fluorescence upon binding, allowing for precise visualization of nucleic acids. Its unique planar structure facilitates stacking interactions with base pairs, influencing the kinetics of fluorescence and providing insights into DNA conformational changes. DAPI's stability and specificity make it a powerful tool for studying nucleic acid dynamics.

1-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide is a fluorescent compound that emits light in the 450-495 nm range, characterized by its unique quinolinium structure. This compound exhibits strong solvatochromism, where its fluorescence properties vary with solvent polarity, indicating distinct molecular interactions. Its ability to form charge-transfer complexes enhances its photophysical behavior, making it a subject of interest in studying electron transfer mechanisms and molecular aggregation phenomena.

Hoechst 33342, UltraPure grade, is a fluorescent dye known for its distinctive binding affinity to DNA, particularly in regions rich in adenine-thymine pairs. This compound exhibits a remarkable ability to intercalate between base pairs, leading to enhanced fluorescence upon binding. Its emission spectrum in the 450-495 nm range is influenced by environmental factors, showcasing unique photostability and sensitivity to pH changes, which can affect its interaction dynamics and fluorescence intensity.

FURA 2/AM is a fluorescent calcium indicator that exhibits a unique ability to bind selectively to calcium ions, resulting in a significant change in fluorescence properties. Its emission spectrum spans 450-495 nm, with a notable shift in intensity upon calcium binding. This compound is characterized by its cell-permeable nature, allowing it to enter live cells and respond to intracellular calcium fluctuations. The kinetics of its fluorescence response are rapid, enabling real-time monitoring of calcium dynamics.

Hoechst 33342 trihydrochloride is a fluorescent dye known for its strong affinity for DNA, particularly binding to the A-T rich regions. It emits fluorescence in the 450-495 nm range, allowing for effective visualization of cellular nuclei. The compound exhibits high photostability and low toxicity, making it suitable for prolonged imaging. Its unique intercalation mechanism enhances specificity, enabling detailed studies of chromatin structure and dynamics in live cells.

MeOSuc-AAPV-AMC is a fluorescent compound that exhibits remarkable photophysical properties, emitting light in the 450-495 nm range. Its structure facilitates specific interactions with target biomolecules, enhancing its sensitivity in detection applications. The compound undergoes rapid reaction kinetics, allowing for real-time monitoring of enzymatic activities. Its unique design promotes efficient energy transfer, making it a valuable tool for studying molecular interactions and dynamics in various environments.

DAPI, dilactate, is a fluorescent dye characterized by its strong affinity for DNA, enabling selective binding to A-T rich regions. This interaction results in a pronounced fluorescence emission within the 450-495 nm range. The compound exhibits high photostability and low background fluorescence, which enhances signal clarity. Its unique molecular structure allows for efficient stacking between DNA bases, facilitating enhanced fluorescence intensity and enabling detailed studies of nucleic acid dynamics.

Hoechst 33258, UltraPure grade, is a fluorescent dye renowned for its selective binding to double-stranded DNA, particularly at A-T rich sequences. This compound exhibits a distinct emission spectrum in the 450-495 nm range, attributed to its unique intercalation properties. Its planar structure allows for effective π-π stacking with nucleobases, enhancing fluorescence intensity. Additionally, Hoechst 33258 demonstrates remarkable photostability, making it suitable for prolonged imaging applications.

Chymotrypsin Substrate II, Fluorogenic, is a specialized compound designed to undergo hydrolysis in the presence of chymotrypsin, resulting in a fluorescent signal. Its unique structure features a peptide bond that selectively interacts with the enzyme, facilitating rapid reaction kinetics. The resulting fluorescence emission in the 450-495 nm range is a direct consequence of the cleavage event, allowing for sensitive detection of enzymatic activity. This substrate's design enhances specificity and sensitivity in biochemical assays.

Hoechst 33258 is a fluorescent dye that exhibits strong binding affinity for double-stranded DNA, enabling it to intercalate between base pairs. This interaction alters its electronic properties, resulting in enhanced fluorescence when excited within the 450-495 nm range. The compound's unique ability to form stable complexes with nucleic acids allows for precise visualization of cellular structures. Its distinct photophysical behavior makes it a valuable tool for studying DNA dynamics and cellular morphology.