Date published: 2025-10-15

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Chromogenic Substrates

Santa Cruz Biotechnology now offers a broad range of Chromogenic Substrates for use in various applications. Chromogenic substrates are specialized compounds designed to produce a visible color change when they undergo specific biochemical reactions, typically involving enzyme activity. These substrates are invaluable in biochemistry and molecular biology for assays that require quick and direct visualization of enzyme presence or activity without the need for advanced imaging equipment. By providing a simple, yet effective means of detecting enzyme activities, chromogenic substrates are widely used in research involving the study of enzymatic pathways, enzyme inhibition, and enzyme kinetics. They are particularly important in fields such as genetics, where they help in identifying gene expression through enzymatic markers, and in microbiology, for identifying bacterial colonies based on their enzymatic properties. Moreover, chromogenic substrates have a critical role in environmental science, facilitating the detection of specific contaminants and pollutants through enzyme-based assays. Their ease of use and the immediate visual feedback they provide make chromogenic substrates a staple in laboratories focusing on cellular and molecular research, providing insights into biochemical processes without the complexities associated with fluorescent or radioactive labeling techniques. This straightforward approach to monitoring enzyme activity has fostered their widespread adoption in non-clinical research settings, enhancing the understanding of numerous biological processes. View detailed information on our available Chromogenic Substrates by clicking on the product name.

Items 21 to 30 of 112 total

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Product NameCAS #Catalog #QUANTITYPriceCitationsRATING

4-Nitrophenyl-α-D- glucopyranoside

3767-28-0sc-281427
sc-281427A
1 g
5 g
$93.00
$251.00
2
(0)

4-Nitrophenyl-α-D-glucopyranoside serves as a chromogenic substrate that undergoes hydrolysis by glycosidases, leading to the release of a yellow chromophore. This transformation is characterized by its distinct molecular interactions, which enhance substrate binding and specificity. The compound's stability in various pH environments allows for consistent reaction kinetics, making it an effective tool for monitoring enzymatic activity in diverse biochemical contexts. Its solubility in water promotes efficient assay performance.

2′-Hydroxy-5′-nitrohexadecanamide

60301-87-3sc-209375
100 mg
$300.00
(0)

2'-Hydroxy-5'-nitrohexadecanamide acts as a chromogenic agent through its unique ability to undergo selective reduction, resulting in a colorimetric change. The compound's long hydrocarbon chain enhances lipophilicity, facilitating interactions with lipid membranes. Its reactivity is influenced by the nitro group, which participates in electron transfer processes, leading to distinct optical properties. This behavior allows for sensitive detection in various analytical applications.

GP-pNA, Chromogenic Substrate

103213-34-9sc-201156
25 mg
$194.00
(1)

GP-pNA serves as a chromogenic substrate by engaging in specific enzymatic reactions that yield a measurable color change. Its structure features a para-nitroaniline moiety, which is pivotal for its interaction with enzymes, promoting rapid hydrolysis. The compound's unique electronic configuration allows for efficient electron delocalization, enhancing its sensitivity in colorimetric assays. This substrate's distinct kinetic profile enables precise monitoring of enzymatic activity, making it a valuable tool in analytical chemistry.

8-Hydroxyquinoline-b-D-galactopyranoside

113079-84-8sc-281503
sc-281503A
1 g
5 g
$150.00
$450.00
(0)

8-Hydroxyquinoline-b-D-galactopyranoside acts as a chromogenic agent through its ability to undergo selective hydrolysis, resulting in a vivid colorimetric response. The presence of the 8-hydroxyquinoline moiety facilitates strong chelation with metal ions, influencing reaction dynamics and enhancing color intensity. Its unique structural features promote specific interactions with enzymes, allowing for tailored kinetic behavior in various assays, thus providing a reliable means of detecting enzymatic activity.

5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt

160369-85-7sc-217158
sc-217158A
10 mg
50 mg
$256.00
$549.00
1
(1)

5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt serves as a chromogenic compound by engaging in specific enzymatic reactions that yield distinct color changes. Its indole structure allows for effective electron delocalization, enhancing the chromogenic response. The compound's unique halogen substitutions contribute to its reactivity, facilitating selective interactions with sialic acid-binding proteins, which can modulate reaction rates and improve sensitivity in detection assays.

Phenyl-β-D-glucuronide

17685-05-1sc-222163
sc-222163A
25 mg
100 mg
$49.00
$141.00
(0)

Phenyl-β-D-glucuronide acts as a chromogenic agent through its ability to undergo hydrolysis, producing a colored product upon enzymatic cleavage. The phenolic group enhances electron donation, promoting resonance stabilization and intensifying color development. Its glucuronide moiety facilitates specific interactions with glucuronidase enzymes, leading to distinct reaction kinetics. This compound's solubility characteristics further influence its reactivity, making it a versatile tool in various analytical applications.

Chymotrypsin Substrate I, Colorimetric

68982-90-1sc-300372
10 mg
$142.00
(0)

Chymotrypsin Substrate I, Colorimetric, serves as a chromogenic substrate by undergoing selective cleavage in the presence of chymotrypsin, resulting in a measurable color change. The aromatic structure enhances light absorption, while the peptide bond's susceptibility to enzymatic hydrolysis dictates the reaction rate. This substrate's unique interactions with the enzyme's active site facilitate precise kinetic measurements, making it an effective indicator in biochemical assays.

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside

107021-38-5sc-278107
1 g
$382.00
(0)

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside acts as a chromogenic compound by undergoing hydrolysis, leading to a vivid colorimetric response. Its indole ring system contributes to distinct electronic properties, allowing for enhanced light absorption upon cleavage. The presence of halogen substituents influences the reactivity and stability of the compound, while the specific glycosidic bond configuration dictates the kinetics of enzymatic interactions, providing a reliable visual cue in biochemical analyses.

Ac-YVAD-pNA

149231-66-3sc-311283
sc-311283A
1 mg
5 mg
$46.00
$112.00
5
(0)

Ac-YVAD-pNA serves as a chromogenic substrate by undergoing enzymatic cleavage, resulting in a measurable color change. Its unique peptide structure, featuring a specific sequence, allows for selective interactions with caspases, facilitating precise monitoring of proteolytic activity. The p-nitroaniline moiety enhances light absorption, contributing to a pronounced colorimetric response. This compound's stability and reactivity are influenced by its amino acid composition, optimizing its performance in biochemical assays.

BCIP/NBT-Blue Liquid Substrate System for Membranes

sc-291929
100 ml
$165.00
17
(1)

BCIP/NBT-Blue Liquid Substrate System is a chromogenic reagent that undergoes a series of enzymatic reactions, leading to the formation of a blue precipitate. The unique interaction between BCIP and alkaline phosphatase generates a highly sensitive colorimetric response. Its distinct electron-donating properties enhance the formation of the colored product, while the stability of the resulting complex ensures reliable detection. This system's kinetic profile allows for rapid visualization, making it ideal for various analytical applications.