Purified Recombinant Proteins

Santa Cruz Biotechnology offers purified recombinant proteins for in vitro research use. Recombinant proteins are created by cloning a fragment of exogenous DNA into a vector. Using molecular cloning technology, recombinant DNA (rDNA) is generated and introduced into a living cell. The rDNA is later transcribed into mRNA and translated by the host machinery. Note the mRNA generated in prokaryotes is considered "mature" and will not go through further processing unlike eukaryotes where the pre-mRNA transcript goes through 5' cap addition, splicing and polyadenylation.