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If I am trying to label this protein using a fluorescent dye like Alexa Fluor etc. Which conjugation chemistry (Conjugation to carboxyl groups (-COOH), Conjugation to amino groups (-NH2) or Conjugation to the thiol groups (-SH)), would be safe to use?
Asked by: Pkl539
Thank you for your question. We would suggest using an amine reative fluorescent dye to start with which should work for this recombinant protein. If it doesn’t work, you could also try dyes that reach -COOH or -SH groups.
Answered by: Technical Support
Date published: 2019-05-03
Can you please let me know which is the tag? Is it a his-tag; HA-tag; His-tag.
If yes, can you please let me know is it at the N-terminus or the C-terminus,Thank you
Asked by: uday M
Thank you for your inquiry. This purified protein is GST tagged at the N-terminus. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com