3830431G21Rik Activators
Chemical activators of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 (PHD1) can initiate a cascade of biochemical events leading to its activation. Phorbol 12-myristate 13-acetate (PMA), known for its ability to activate protein kinase C (PKC), initiates phosphorylation events that are critical for the functional state of PHD1. Similarly, DiC8, a synthetic analog of diacylglycerol (DAG), can also target PKC, resulting in the phosphorylation and subsequent activation of PHD1. Interestingly, BIM, primarily a PKC inhibitor, can at low concentrations indirectly contribute to the activation of PKC and thereby PHD1. Moreover, Forskolin, by elevating intracellular cAMP levels, leads to the activation of protein kinase A (PKA), which in turn can phosphorylate PHD1. A related compound, dibutyryl-cAMP (db-cAMP), acts as a cAMP analog to activate PKA, providing another pathway for PHD1 activation.
Calcium signaling also plays a pivotal role in the activation of PHD1. Ionomycin and A23187, both of which increase intracellular calcium levels, can activate calmodulin-dependent kinase (CaMK), which then may phosphorylate PHD1. Thapsigargin, by inhibiting the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), causes a rise in intracellular calcium that can also trigger activation pathways involving PHD1. Additionally, the perturbation of phosphorylation equilibrium by calyculin A and okadaic acid, both phosphatase inhibitors, helps in sustaining the phosphorylated, and thus active, state of PHD1. Lastly, anisomycin, through the activation of stress-activated protein kinases, and bryostatin 1, by modulating the activity of PKC, can contribute to the phosphorylation and activation of PHD1, illustrating the diverse mechanisms by which this protein can be regulated.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC) which can enhance the phosphorylation status of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1, leading to its functional activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin increases intracellular calcium levels, which can activate calmodulin-dependent kinase (CaMK), potentially leading to the activation of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 through phosphorylation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $160.00 $750.00 | 59 | |
Calyculin A is a phosphatase inhibitor that prevents dephosphorylation of proteins, which can sustain or enhance the activation state of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 by maintaining its phosphorylation levels. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid, another phosphatase inhibitor, can also maintain the phosphorylation state of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1, which is essential for its activation. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin raises intracellular calcium levels by inhibiting the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), which can activate downstream kinases like CaMK and potentially lead to the activation of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $45.00 $130.00 $480.00 $4450.00 | 74 | |
db-cAMP is a cAMP analog that can activate PKA. PKA then phosphorylates target proteins, which can include pleckstrin homology domain containing, family D (with coiled-coil domains) member 1, leading to its activation. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $54.00 $128.00 $199.00 $311.00 | 23 | |
A23187 is an ionophore that increases intracellular calcium levels, activating calcium-dependent kinases and potentially leading to the activation of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1. | ||||||
1,2-Dioctanoyl-sn-glycerol | 60514-48-9 | sc-202397 sc-202397A | 10 mg 50 mg | $46.00 $249.00 | 2 | |
DiC8 is a synthetic diacylglycerol (DAG) analog which activates PKC. Activated PKC can phosphorylate pleckstrin homology domain containing, family D (with coiled-coil domains) member 1, leading to its activation. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $103.00 $237.00 | 36 | |
BIM is a PKC inhibitor, but it can paradoxically activate PKC at low concentrations. This can lead to the activation of pleckstrin homology domain containing, family D (with coiled-coil domains) member 1 through phosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that can activate stress-activated protein kinases (SAPKs). SAPKs can phosphorylate various substrates, potentially including pleckstrin homology domain containing, family D (with coiled-coil domains) member 1, leading to its activation. | ||||||