Date published: 2025-9-6

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USP14 抗体 (F-4): sc-398009

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说明书
  • USP14 抗体 F-4 是小鼠单克隆 IgG1 κ,USP14抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列195-494位于human物种的USP14的C-terminus
  • USP14 抗体 (F-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 USP14; 也和以下物种反应,包括: canine
  • 抗USP14抗体(F-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取USP14 (F-4): sc-398009免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 USP14 抗体 (F-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与USP14 抗体 (F-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

USP14抗体(F-4)是一种IgG1 κ小鼠单克隆USP14抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的USP14蛋白。USP14抗体(F-4)既可作为非偶联的抗USP14抗体形式提供,也可作为多种偶联形式的抗USP14抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。泛素(Ub)途径涉及三个连续的酶促步骤,这些步骤有助于将Ub和类Ub分子与特定的蛋白质底物结合。泛素途径通过使用可以添加或去除泛素的多种酶,控制许多细胞内过程,如信号转导、转录激活和细胞周期进程。USP14(泛素特异性肽酶14),也称为TGT(tRNA-鸟嘌呤转糖基酶),是一种细胞质蛋白,属于去泛素化酶的泛素特异性处理家族。USP14在细胞内以同源二聚体的形式存在,其功能是从泛素化蛋白质和泛素融合前体中裂解泛素残基,从而使这些蛋白质免于蛋白酶体降解。在小鼠中,编码USP14的基因缺陷或突变会导致生长迟缓或胎儿死亡,这表明USP14在早期发育过程中起着关键作用。由于选择性剪接事件,USP14表达了多种异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP14 抗体 (F-4) 参考文献:

  1. USP14 通过其在肝细胞癌中的去泛素化活性维持 HIF1-α 的稳定。  |  Lv, C., et al. 2021. Cell Death Dis. 12: 803. PMID: 34420039
  2. 通过时间分辨冷冻电镜观察 USP14 调节的人类蛋白酶体异构体。  |  Zhang, S., et al. 2022. Nature. 605: 567-574. PMID: 35477760
  3. 去泛素化酶 USP14 稳定的 MFG-E8 可抑制支气管上皮细胞中由香烟烟雾诱导的铁突变。  |  Cui, Y., et al. 2023. Cell Death Dis. 14: 2. PMID: 36596780
  4. USP14 通过靶向稳定 JNK 促进结直肠癌的进展。  |  Du, XH., et al. 2023. Cell Death Dis. 14: 56. PMID: 36693850
  5. USP14 稳定 SDC2 通过与 PDK1 协同作用促进胃癌进展  |  You, L., et al. 2023. Int J Biol Sci. 19: 3483-3498. PMID: 37496999
  6. 用b-AP15靶向蛋白酶体去泛素酶USP14和UCHL5可降低结直肠癌细胞对5-氟尿嘧啶的耐药性。  |  Ding, W., et al. 2023. Acta Pharmacol Sin. 44: 2537-2548. PMID: 37528233
  7. USP14 在肝细胞癌中表现出较高的表达水平,并通过 HK2/AKT/P62 轴在促进肝癌细胞生长方面发挥着至关重要的作用。  |  Zhang, N., et al. 2024. BMC Cancer. 24: 237. PMID: 38383348
  8. USP14 通过介导铁变态反应提高视网膜母细胞瘤对顺铂的敏感性。  |  Liu, H., et al. 2024. Naunyn Schmiedebergs Arch Pharmacol. 397: 8671-8680. PMID: 38819674
  9. USP14 缺乏可通过降解 Skp2 蛋白抑制血管损伤后的新生内膜形成。  |  Xia, X., et al. 2024. Cell Death Discov. 10: 295. PMID: 38909015
  10. USP14 抑制剂可促进 DNA 损伤修复,抑制卵巢早衰患者卵巢颗粒细胞的衰老。  |  Ma, LZ., et al. 2024. J Transl Med. 22: 834. PMID: 39261935

订购信息

产品名称产品编号规格价格数量收藏夹

USP14 抗体 (F-4)

sc-398009
200 µg/ml
$316.00

USP14 (F-4): m-IgG Fc BP-HRP 套装

sc-527729
200 µg Ab; 10 µg BP
$354.00

USP14 (F-4): m-IgG1 BP-HRP 套装

sc-533102
200 µg Ab; 20 µg BP
$354.00

USP14 抗体 (F-4) AC

sc-398009 AC
500 µg/ml, 25% agarose
$416.00

USP14 抗体 (F-4) HRP

sc-398009 HRP
200 µg/ml
$316.00

USP14 抗体 (F-4) FITC

sc-398009 FITC
200 µg/ml
$330.00

USP14 抗体 (F-4) PE

sc-398009 PE
200 µg/ml
$343.00

USP14 抗体 (F-4) Alexa Fluor® 488

sc-398009 AF488
200 µg/ml
$357.00

USP14 抗体 (F-4) Alexa Fluor® 546

sc-398009 AF546
200 µg/ml
$357.00

USP14 抗体 (F-4) Alexa Fluor® 594

sc-398009 AF594
200 µg/ml
$357.00

USP14 抗体 (F-4) Alexa Fluor® 647

sc-398009 AF647
200 µg/ml
$357.00

USP14 抗体 (F-4) Alexa Fluor® 680

sc-398009 AF680
200 µg/ml
$357.00

USP14 抗体 (F-4) Alexa Fluor® 790

sc-398009 AF790
200 µg/ml
$357.00

I did a blast using human USP14 against the Drosophila USP14, and the similarity between sequences is 55.3%. I would like to know if there would be a possibility of getting a small amount to test this antibody (USP14 Antibody (F-4): sc-398009) in flies.

Asked by: Anonymous
Thank you for your question. Please get in touch with our technical service department to arrange for a free sample of this antibody. Please be aware though, that sequence identity of 55% is very low for monoclonal antibodies and the product is very unlikely to recognize a protein with such a comparatively low level of sequence conservation.
Answered by: Technical Support Europe
Date published: 2020-10-21

The website only cites that "this antibody raised against amino acids 195-494 mapping at the C-terminus of USP14". I'm wondering whether the detailed amino acid targeting map of this monoclonal antibody is reachable?

Asked by: echo yan
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, USP14 (F-4): sc-398009, has not been determined.
Answered by: Technical Support
Date published: 2017-10-02

I am using USP14 (F-4): sc-398009 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 产品很棒试用装的抗体产品很不错,给了我很大的实验帮助。。。。。。。。。。。。。。。。。。。。。。。。。。。。。
Date published: 2024-10-14
Rated 5 out of 5 by from Fine for WBTested in SHSY-5Y human neuroblastoma cells (~15ug/lane), dilution 1:1000 in 3% milk in TBST. Got a single clear band at expected size.
Date published: 2018-05-15
Rated 5 out of 5 by from goodI am able to detect endogenous USP14 in 293 cell lysates.
Date published: 2017-08-04
Rated 5 out of 5 by from Satisfactory Western Blot data of USP14 expressionSatisfactory Western Blot data of USP14 expression in HeLa and MOLT-4 whole cell lysates. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Nice Western Blot data of USP14 expression in UNice Western Blot data of USP14 expression in U-251-MG, SK-OV-3 and HCT-116 whole cell lysates. -SCBT QC
Date published: 2013-04-08
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USP14 抗体 (F-4) is rated 5.0 out of 5 by 5.
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