Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
UCP4 Antibody (A-5) - Western Blotting - Image 139095
UCP4 抗体 (A-5): sc-365295. SK-N-SH (A) 和 U-87 MG (B) 全细胞裂解液中 UCP4 表达的 Western 印迹分析.
UCP4 Antibody (A-5) - Western Blotting - Image 139095
UCP4 抗体 (A-5): sc-365295. SK-N-SH (A) 和 U-87 MG (B) 全细胞裂解液中 UCP4 表达的 Western 印迹分析.
UCP4 Antibody (A-5) - Immunohistochemistry - Image 161017
UCP4 抗体 (A-5): sc-365295. 福尔马林固定, 石蜡包埋的人小肠组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
UCP4 抗体 (A-5): sc-365295. SK-N-SH (A) 和 U-87 MG (B) 全细胞裂解液中 UCP4 表达的 Western 印迹分析.

UCP4 抗体 (A-5): sc-365295

4.3(8)
寫評論提問

说明书
  • UCP4 抗体 A-5 是小鼠单克隆 IgM κ，UCP4抗体, 在5篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的UCP4的N-terminus的氨基酸17-37之间
  • 推荐用于 mouse, rat 和 human 来源的UCP4 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取UCP4 (A-5): sc-365295的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成UCP4 抗体 (A-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 UCP 抗体 包括 UCP1, UCP2和UCP4

UCP4抗体（A-5）是一种IgMκ小鼠单克隆UCP4抗体（也称为SLC25A27抗体），能够通过WB、IP、IF、IHC(P)和ELISA方法检测来自小鼠、大鼠和人类的UCP4蛋白。UCP4抗体（A-5）以非偶联型抗UCP4抗体形式供应。内温动物的代谢率中有一大部分可归因于对解偶联作用的抵消，在这种作用中，质子沿电化学梯度流动，独立于ATP的产生而生成热量。解偶联作用在产热棕色脂肪组织中尤为明显，这种组织表达组织特异性解偶联蛋白（UCP），这表明固有解偶联作用和代谢率受到UCPs的调控。UCPs是一组线粒体转运蛋白，参与体温调节性产热和基础代谢率的维持。UCP家族中，有一个大脑特异性的新成员--UCP4，它与UCP3最为相关，并具备线粒体转运蛋白的特征。与其他已知的UCPs不同，UCP4的mRNA在胎儿和成年脑组织中均有表达。人类UCP4是一种323个氨基酸的蛋白质，由于其早期在进化树上的出现，人们推测它可能参与凋亡过程。在急性寒冷暴露下，大脑UCP4的mRNA增加了1.5倍，这表明UCP4参与组织特异性体温调节和代谢变化。UCP特异性序列位于第一、第二和第四个α螺旋中，它们参与脂肪酸阴离子的结合和转位。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

UCP4 抗体 (A-5) 参考文献:

  1. UCP4 是一种新型脑特异性线粒体蛋白，可降低哺乳动物细胞的膜电位。  |  Mao, W., et al. 1999. FEBS Lett. 443: 326-30. PMID: 10025957
  2. 解偶联蛋白同源物：新出现的生理功能观点。  |  Adams, SH. 2000. J Nutr. 130: 711-4. PMID: 10736318
  3. 线粒体解偶联蛋白基序的特定序列。  |  Jezek, P. and Urbánková, E. 2000. IUBMB Life. 49: 63-70. PMID: 10772343
  4. 新型 UCP5/BMCP1 同工型的特征以及通过饮食或温度调节对 UCP4 和 UCP5 表达的不同调控。  |  Yu, XX., et al. 2000. FASEB J. 14: 1611-8. PMID: 10928996
  5. 线粒体解偶联蛋白与系统发育--UCP4作为祖先解偶联蛋白  |  Hanák, P. and Jezek, P. 2001. FEBS Lett. 495: 137-41. PMID: 11334880
  6. 解偶联蛋白在病理生理状态中的作用。  |  Argilés, JM., et al. 2002. Biochem Biophys Res Commun. 293: 1145-52. PMID: 12054495
  7. 突触可塑性和神经细胞死亡中的线粒体钾通道和解偶联蛋白  |  Mattson, MP. and Liu, D. 2003. Biochem Biophys Res Commun. 304: 539-49. PMID: 12729589
  8. 线粒体解偶联蛋白的进化：新型无脊椎动物 UCP 同源物表明 UCP 家族的早期进化分化。  |  Sokolova, IM. and Sokolov, EP. 2005. FEBS Lett. 579: 313-7. PMID: 15642337
  9. NF-κB对UCP4的转录调控及其在介导对MPP+毒性的保护中的作用。  |  Ho, JW., et al. 2010. Free Radic Biol Med. 49: 192-204. PMID: 20385226
  10. UCP4的过度表达可改善L6肌细胞的脂肪酸氧化和胰岛素敏感性。  |  Gao, CL., et al. 2011. J Bioenerg Biomembr. 43: 109-18. PMID: 21607879
  11. 解偶联蛋白-4（UCP4）通过与神经母细胞瘤细胞线粒体复合体 II 相互作用来增加 ATP 供应。  |  Ho, PW., et al. 2012. PLoS One. 7: e32810. PMID: 22427795
  12. 小脑浦肯野细胞的 Ucp4 敲除诱导缓激肽。  |  Wang, YY., et al. 2024. Mol Neurobiol. 61: 1119-1139. PMID: 37688710

订购信息

产品名称产品编号规格价格数量收藏夹

UCP4 抗体 (A-5)

sc-365295
200 µg/ml
$316.00

UCP4 (A-5) 中和勝肽

sc-365295 P
100 µg/0.5 ml
$68.00

Hi there, I have ordered UCP4 Antibody (A-5): sc-365295 and a blocking peptide and safely received that. I was wondering how we can store these materials for long time (like more than a year). Is it OK to keep in 4 C in fridge?

Asked by: Marzieh
Thank you for your question. You can find recommended storage conditions on the product's datasheet. This antibody, and most others, should be kept at +4 °C for short- and long-term storage.
Answered by: Tech Support Europe
Date published: 2021-01-18

What application is the blocking peptide sc-365295 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_365295, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 118ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Segnale non rilevabileHo testato la aliquota di prova in Western Blot. Caricati 40ug di corteccia cerebrale di topi WT e KO per il gene CDKL5 (P70), anticorpo utilizzato 1:500 in milk 5% e sviluppato prima con Westar Sun (Cyanagen Cod. XLS0623,0250) e poi con Westar Supernova (Cyanagen Cod. XLS3,0100). Purtroppo il segnalo risulta assente e, pertanto, non quantificabile.
Date published: 2020-06-08
Rated 5 out of 5 by from WBUCP4(dilution 1 :500)work well , and get the right band
Date published: 2017-12-11
Rated 5 out of 5 by from good specificityI recommend loading 20ug antibody and diluting it at 1:200 for WB. I recommend this antibody with good results.
Date published: 2017-11-01
Rated 4 out of 5 by from Antibody detects UCP4 band in pig heart lysate.Ab was used on membrane protein lysate from heart of mini pigs and gives a clean band at the expected MW. Best dilution for WB is 1/200. I used PVDF membrane.
Date published: 2017-09-08
Rated 5 out of 5 by from good signalsHuman tissue by WB use this antibody, 1:500 dilution, get the right band.
Date published: 2017-03-28
Rated 4 out of 5 by from UCP4 A-5I have used this antibody on F9 cell lysate and it works okay at 1:100 dilution. I do see some non-specific bands with this antibody though, but definitely works!
Date published: 2016-12-12
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Good Western Blot data of UCP4 expression in SKGood Western Blot data of UCP4 expression in SK-N-SH and U-87 MG whole cell lysates. -SCBT QC
Date published: 2014-07-31
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_365295, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
UCP4 抗体 (A-5) is rated 4.3 out of 5 by 8.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_365295, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。