Date published: 2025-9-6
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Topo IIα Antibody (F-12) - Western Blotting - Image 356558
Topo IIα 抗体 (F-12): sc-365916. HeLa (A), Jurkat (B) 和 MM-142 (C) 核提取物及 RPE-J 全细胞裂解液 (D) 中 Topo IIα 表达的 Western 印迹分析.
Topo IIα 抗体 (F-12): sc-365916. HeLa (A), Jurkat (B) 和 MM-142 (C) 核提取物及 RPE-J 全细胞裂解液 (D) 中 Topo IIα 表达的 Western 印迹分析.

Topo IIα 抗体 (F-12): sc-365916

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说明书
  • Topo II alpha 抗体 F-12 是小鼠单克隆 IgG1 κ，Topo IIα抗体, 在25篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Topo IIα的C-terminus的氨基酸1511-1530之间
  • Topo II alpha 抗体 (F-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Topo IIα; 也和以下物种反应，包括： porcine
  • 抗Topo II alpha抗体（F-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Topo IIα (F-12): sc-365916的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Topo IIα 抗体 (F-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Topo IIα 抗体 (F-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Topo 抗体 包括 Topo I, Topo II, Topo II α, Topo II β和Topo III β-1

Topo IIα抗体（F-12）是一种IgG1 κ小鼠单克隆Topo IIα抗体（也称为TOP2A抗体、Topoisomerase IIα抗体或DNA Topoisomerase IIα抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Topo IIα蛋白。Topo IIα抗体（F-12）既可作为非偶联抗Topo IIα抗体形式，也可作为多种偶联形式的抗Topo IIα抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。DNA拓扑异构酶I和II（Topo I和Topo II）是核酶，通过暂时断裂和重新连接DNA链来调节真核细胞中DNA的拓扑结构。真核生物的拓扑异构酶能够松弛正超螺旋和负超螺旋，而原核生物的拓扑异构酶只能松弛负超螺旋。DNA拓扑异构酶在DNA复制、重组和转录中发挥作用，并已被确定为许多抗癌药物的目标。Topo I是一种普遍表达的可溶性酶，通过在DNA的一条链上引入一个短暂的断裂来发挥作用，而Topo II则通过制造一个短暂的双链断裂来发挥作用。Topo II由两个不同的基因编码，生成两个不同的异构体，分别被指定为Topo IIα和Topo IIβ。Topo IIβ和Topo IIα在其N端四分之三处大致同源，然而，C端片段差异较大，这表明这些区域可能介导不同的细胞功能，并解释了观察到的两种异构体的不同组织表达模式。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Topo IIα 抗体 (F-12) 参考文献:

  1. 拓扑异构酶 II alpha 和拓扑异构酶 II beta 基因：特性分析及分别与人类第 17 和 3 号染色体的映射关系。  |  Tan, KB., et al. 1992. Cancer Res. 52: 231-4. PMID: 1309226
  2. 人类 I 型 DNA 拓扑异构酶基因的结构。  |  Kunze, N., et al. 1991. J Biol Chem. 266: 9610-6. PMID: 1851751
  3. 从 HeLa 细胞中分离并鉴定编码新型 DNA 拓扑异构酶 II 同源物的人类 cDNA 克隆。  |  Austin, CA. and Fisher, LM. 1990. FEBS Lett. 266: 115-7. PMID: 2163884
  4. 编码两种人类 DNA 拓扑异构酶 II 同工酶的 cDNA 克隆的特征和免疫学鉴定。  |  Chung, TD., et al. 1989. Proc Natl Acad Sci U S A. 86: 9431-5. PMID: 2556712
  5. 人 DNA 拓扑异构酶 I 的 cDNA 克隆：67.7-kDa 羧基端片段的催化活性。  |  D'Arpa, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 2543-7. PMID: 2833744
  6. DNA 拓扑异构酶的机制。  |  Roca, J. 1995. Trends Biochem Sci. 20: 156-60. PMID: 7770916
  7. 人类拓扑异构酶 I 的机制模型。  |  Stewart, L., et al. 1998. Science. 279: 1534-41. PMID: 9488652

订购信息

产品名称产品编号规格价格数量收藏夹

Topo IIα 抗体 (F-12)

sc-365916
200 µg/ml
$316.00

Topo IIα (F-12): m-IgG Fc BP-HRP 套装

sc-529510
200 µg Ab; 10 µg BP
$354.00

Topo IIα (F-12): m-IgGκ BP-HRP 套装

sc-522420
200 µg Ab, 40 µg BP
$354.00

Topo IIα (F-12): m-IgG1 BP-HRP 套装

sc-543552
200 µg Ab; 20 µg BP
$354.00

Topo IIα 抗体 (F-12) AC

sc-365916 AC
500 µg/ml, 25% agarose
$416.00

Topo IIα 抗体 (F-12) HRP

sc-365916 HRP
200 µg/ml
$316.00

Topo IIα 抗体 (F-12) FITC

sc-365916 FITC
200 µg/ml
$330.00

Topo IIα 抗体 (F-12) PE

sc-365916 PE
200 µg/ml
$343.00

Topo IIα 抗体 (F-12) Alexa Fluor® 488

sc-365916 AF488
200 µg/ml
$357.00

Topo IIα 抗体 (F-12) Alexa Fluor® 546

sc-365916 AF546
200 µg/ml
$357.00

Topo IIα 抗体 (F-12) Alexa Fluor® 594

sc-365916 AF594
200 µg/ml
$357.00

Topo IIα 抗体 (F-12) Alexa Fluor® 647

sc-365916 AF647
200 µg/ml
$357.00

Topo IIα 抗体 (F-12) Alexa Fluor® 680

sc-365916 AF680
200 µg/ml
$357.00

Topo IIα 抗体 (F-12) Alexa Fluor® 790

sc-365916 AF790
200 µg/ml
$357.00

Topo IIα (F-12) 中和勝肽

sc-365916 P
100 µg/0.5 ml
$68.00

Can the peptide sc-365916 P be used in Western Blot as a positive control?

Asked by: AbPolly
Thank you for your question. The blocking peptide sc-365916 P is a short synthetic peptide intended for use as a negative control by preadsorption. It is not appropriate for use as a positive control in Western Blot.
Answered by: Technical Service
Date published: 2017-02-02
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Rated 4 out of 5 by from Western Blot on canine cell linesWorked well in canine prostate and bladder cancer cell lines; 1:200 in Western Blot, could be reused several times.
Date published: 2023-07-05
Rated 3 out of 5 by from GoodGood, though weak, signal in WB of total lysates of human cell lines.
Date published: 2020-05-26
Rated 5 out of 5 by from TOP2AThis antibody had strong band in WB in human cell lines
Date published: 2019-10-23
Rated 5 out of 5 by from Nice band in WBTopo IIα had strong band in WB in human prostate cancer cell line LNcap
Date published: 2018-07-09
Rated 3 out of 5 by from Results were not very clearGastric cancer cells BGC823 and BGC8230shRNA-trop2 were uesd to confirm the expression of Topo IIβ. Transfering time was 360 minutes in 250mA. Blocking in milk for hour. Incubateing with first antibody(1:500) overnight. Incubateing with second antibody(1:2000) for one hour. After several try，results were blank or not clear
Date published: 2017-11-18
Rated 5 out of 5 by from a good antibody for both WB and IFThis antibody generates a signal at the predicted position on the western blot (1:500 dilution, 40ug HeLa extract). It also stains the nucleus with a stronger signal in the nucleoli (1:200, formaldehyde-fixed HeLa)
Date published: 2017-09-01
Rated 5 out of 5 by from GreatThe antibody is pretty good for IF staining. The background is very low.
Date published: 2017-08-02
Rated 5 out of 5 by from strong IF stainingWe used this antibody for IF staining in nuclear regions of HeLa cells and got a very strong intensity from the antibody
Date published: 2017-01-04
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Topo IIα 抗体 (F-12) is rated 4.3 out of 5 by 12.
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