TOB1 Antibody (D-7): sc-136969

TOB1 Antibody (D-7) is a mouse monoclonal IgG2a light chain antibody that detects TOB1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TOB1 is a critical anti-proliferative protein that plays a significant role in regulating cell cycle progression, particularly in the transition from the G0/G1 phase to the S phase, by interacting with the mammalian homologue of yeast Caf1. This function is vital for maintaining proper cellular growth and preventing uncontrolled proliferation, which is particularly important in the context of cancer biology. Additionally, TOB1 is involved in the modulation of immune responses, as TOB1 can interact with Smad2/4 in T lymphocytes, enhancing SMAD DNA binding to the interleukin-2 promoter and subsequently inhibiting IL-2 transcription. The phosphorylation of TOB1 at specific serine residues by ERK1/2 upon growth-factor stimulation further underscores TOB1′s regulatory role in cellular signaling pathways. Anti-TOB1 antibody (D-7) is an essential tool for researchers studying cell cycle dynamics, immune regulation, and the molecular mechanisms underlying various diseases, including cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TOB1 Antibody (D-7) References:

1. Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2.  |  Shapouri, F., et al. 2016. Cell Tissue Res. 364: 443-51. PMID: 26662055
2. TOB1 Deficiency Enhances the Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Tendon-Bone Healing in a Rat Rotator Cuff Repair Model.  |  Gao, Y., et al. 2016. Cell Physiol Biochem. 38: 319-29. PMID: 26824451
3. Decreased TOB1 expression and increased phosphorylation of nuclear TOB1 promotes gastric cancer.  |  Guan, R., et al. 2017. Oncotarget. 8: 75243-75253. PMID: 29088861
4. Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.  |  Wang, D., et al. 2019. Am J Transl Res. 11: 5227-5239. PMID: 31497236
5. TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer.  |  Bai, Y., et al. 2020. Cancer Med. 9: 1503-1514. PMID: 31891232
6. TOB1 Blocks Intestinal Mucosal Inflammation Through Inducing ID2-Mediated Suppression of Th1/Th17 Cell Immune Responses in IBD.  |  Lin, R., et al. 2022. Cell Mol Gastroenterol Hepatol. 13: 1201-1221. PMID: 34920145
7. Involvement of TOB1 on autophagy in gastric cancer AGS cells via decreasing the activation of AKT/mTOR signaling pathway.  |  Wang, D., et al. 2022. PeerJ. 10: e12904. PMID: 35186488
8. The Overexpression of TOB1 Induces Autophagy in Gastric Cancer Cells by Secreting Exosomes.  |  Wang, Y., et al. 2022. Dis Markers. 2022: 7925097. PMID: 35465266
9. Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.  |  Dong, Z., et al. 2023. Dig Dis Sci. 68: 1316-1331. PMID: 36002674
10. TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression.  |  Yu, Z., et al. 2022. Commun Biol. 5: 943. PMID: 36085336

The preferred TOB1 antibody is TOB1 Antibody (E-1): sc-133095.