Date published: 2025-9-7

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Thy-1/CD90 抗体 (aTHy-1A1): sc-53456

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说明书
  • Thy-1/CD90 抗体 aTHy-1A1 是小鼠单克隆 IgG2a κ,Thy-1/CD90抗体, 在36篇文献中引用,规格为200 µg/ml
  • 针对纯化的Thy-1 human 起源
  • Thy-1/CD90 抗体 (aTHy-1A1) 推荐用于 WB, IP, IF, IHC(P) 和 FCM,检测mouse, rat 和human 来源的 Thy-1
  • 抗Thy-1/CD90抗体(aTHy-1A1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Thy-1/CD90 (aTHy-1A1): sc-53456免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 Thy-1/CD90 抗体 (aTHy-1A1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Thy-1/CD90 抗体 (aTHy-1A1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Thy-1抗体(aTHy-1A1)是一种鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测小鼠、大鼠和人类来源的Thy-1蛋白。抗Thy-1抗体(aTHy-1A1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Thy-1,也称为CD90,是一种以糖基磷脂酰肌醇为锚点的细胞表面糖蛋白,在细胞粘附和信号传导中起着至关重要的作用。Thy-1主要表达于T细胞、神经元和某些干细胞的表面,参与调节免疫反应和维持干细胞特性。造血干细胞上的Thy-1表达,尤其是与CD34共表达时,有助于识别具有多能性的亚细胞群,这对于有效的造血和组织再生至关重要。此外,Thy-1阳性细胞与移植物抗宿主病的发病机制有关,这凸显了Thy-1在移植免疫学和潜在治疗应用中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Thy-1/CD90 抗体 (aTHy-1A1)

sc-53456
200 µg/ml
$316.00

Thy-1/CD90 (aTHy-1A1): m-IgG Fc BP-HRP 套装

sc-525599
200 µg Ab; 10 µg BP
$354.00

Thy-1/CD90 (aTHy-1A1): m-IgG2a BP-HRP 套装

sc-546333
200 µg Ab; 10 µg BP
$354.00

Thy-1/CD90 抗体 (aTHy-1A1) AC

sc-53456 AC
500 µg/ml, 25% agarose
$416.00

Thy-1/CD90 抗体 (aTHy-1A1) HRP

sc-53456 HRP
200 µg/ml
$316.00

Thy-1/CD90 抗体 (aTHy-1A1) FITC

sc-53456 FITC
200 µg/ml
$330.00

Thy-1/CD90 抗体 (aTHy-1A1) PE

sc-53456 PE
200 µg/ml
$343.00

Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor® 488

sc-53456 AF488
200 µg/ml
$357.00

Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor® 546

sc-53456 AF546
200 µg/ml
$357.00

Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor® 594

sc-53456 AF594
200 µg/ml
$357.00

Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor® 647

sc-53456 AF647
200 µg/ml
$357.00

Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor® 680

sc-53456 AF680
200 µg/ml
$357.00

Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor® 790

sc-53456 AF790
200 µg/ml
$357.00

请问这个抗体适用于哪些细胞株?

Asked by: ljy123
Thanks for your question. Thy-1/CD90 Antibody (aTHy-1A1) is recommended for detection of Thy-1 of mouse, rat and human origin. CTLL-2 cell lysate, IMR-32 cell lysate or CCRF-CEM cell lysate can be used as Positive Controls. 感谢您的提问。Thy-1/CD90 抗体 (aTHy-1A1) 推荐用于检测mouse, rat 和human 来源的 Thy-1。CTLL-2 cell lysate, IMR-32 cell lysate or CCRF-CEM cell lysate 可以作为阳性对照细胞系。
Answered by: Yanru Tao
Date published: 2023-05-05

Can Thy-1 (aTHy-1A1): sc-53456 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Thy-1 (aTHy-1A1): sc-53456 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IF for human Mesenchymal Stem Cells is great!Received a sample of this antibody two months ago and it has been excellent for IF for human mesenchymal stem cells. Tried 1:50 and it was great! Definitely purchasing!
Date published: 2020-03-10
Rated 4 out of 5 by from antibody acceptableuse dilution 1: 150 over night for immunofluoresce
Date published: 2018-04-10
Rated 4 out of 5 by from Equine dermal cells are negativeGot sample of this item and tried to use on my equine dermal cells (fibroblast cell line). The procedure: triton X-100 for permeabilization for 30 min, followed by blocking for 30 min. Then, 1:20 dilution of this Ab for 1h at room temperature, followed by standard protocol of immunohistochemical staining with ABC kit and AEC chromogen. Cells were totally negative. But these cells are strongly positive for vimentin v9.
Date published: 2017-12-10
Rated 3 out of 5 by from 阴性申请了试用装,态度很好,速度很快,但是大鼠眼球切片节细胞染色为阴性,免疫荧光,1:200,不知为什么
Date published: 2017-10-09
Rated 4 out of 5 by from Positive signal in WB in mouse PBL wholePositive signal in WB in mouse PBL whole cell lysate. -SCBT QC
Date published: 2015-06-19
Rated 4 out of 5 by from Published for IHC and ELISA in human liverPublished for IHC and ELISA in human liver cancer tissue. -SCBT Publication Review
Date published: 2015-05-15
Rated 4 out of 5 by from Good publishable data in flow cytometry inGood publishable data in flow cytometry in human tooth germ stem cells. -SCBT Publication Review
Date published: 2014-07-18
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Thy-1/CD90 抗体 (aTHy-1A1) is rated 4.0 out of 5 by 7.
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