Date published: 2025-9-8

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Thrombospondin 1 抗体 (A6.1): sc-59887

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说明书
  • Thrombospondin 1抗体(A6.1)是小鼠单克隆IgG1 κ, 在66篇文献中引用,规格为200 µg/ml
  • 针对human来源的还原和烷基化Thrombospondin 1
  • 抗-Thrombospondin 1 抗体 (A6.1) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat, human, equine, bovine, porcine 和canine 来源的 reduced and non-reduced Thrombospondin 1
  • Thrombospondin 1抗体(A6.1)可与Alexa Fluor® 546Alexa Fluor® 594结合,用于WB(RGB)、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Thrombospondin 1 (A6.1): sc-59887免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Thrombospondin 1 抗体 (A6.1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Thrombospondin 1 抗体 (A6.1) 组合成套装提供。 (请参阅下面的订购信息)。

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血栓素蛋白1抗体(A6.1)是一种小鼠单克隆IgG1 kappa轻链抗体,经过工程改造,能够特异性识别血栓素蛋白1(TSP-1)。抗血栓素蛋白1抗体(A6.1)是针对还原和烷基化的人血栓素蛋白1产生的。血栓素蛋白1是一种关键的细胞外糖蛋白,参与关键的细胞间和细胞与基质间的信号传导通路,这些通路对于血小板聚集、细胞粘附、迁移以及组织发育和修复的调节等过程至关重要。通过调节细胞与细胞外基质之间的相互作用,血栓素原1在抑制血管生成、调节伤口愈合以及促进癌细胞转移方面发挥着重要作用,凸显其在生理和病理环境中的重要性。血栓素蛋白单克隆抗体(A6.1)推荐用于多种应用,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP),并显示出对来自小鼠、大鼠、人、马、牛、猪和犬的血栓素蛋白的强物种反应性。抗血栓素蛋白1抗体(A6.1)不会与其他蛋白质(如纤维蛋白原、纤维蛋白原或血管性血友病因子)发生交叉反应,从而确保对血栓素蛋白1的检测可靠、准确。抗血栓素蛋白1抗体(A6.1)适用于研究血栓素蛋白1在维持细胞稳态、应对组织损伤以及了解其在各种疾病机制中的参与方面所起的多方面作用的研究人员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Thrombospondin 1 抗体 (A6.1) 参考文献:

  1. 凝血酶原蛋白 1 和凝血酶原蛋白 2 以同源和异源三聚体的形式表达。  |  O'Rourke, KM., et al. 1992. J Biol Chem. 267: 24921-4. PMID: 1459996
  2. 凝血酶原蛋白 II:凝血酶原蛋白基因家族第二位成员在人类中的部分 cDNA 序列, 染色体位置和表达。  |  LaBell, TL., et al. 1992. Genomics. 12: 421-9. PMID: 1559694
  3. 凝血酶原的生理学  |  Mosher, DF. 1990. Annu Rev Med. 41: 85-97. PMID: 1691903
  4. 小鼠基因组中还有第二个表达的血栓软蛋白基因(Thbs2)。  |  Bornstein, P., et al. 1991. J Biol Chem. 266: 12821-4. PMID: 1712771
  5. 血栓素原蛋白1与年龄相关性黄斑变性的发病机制。  |  Housset, M. and Sennlaub, F. 2015. J Ocul Pharmacol Ther. 31: 406-12. PMID: 26062001
  6. 人类血栓素原蛋白1对革兰氏阳性细菌发病机制的贡献。  |  Binsker, U., et al. 2019. J Innate Immun. 11: 303-315. PMID: 30814475
  7. 通过蛋白酶体抑制血栓素原蛋白1诱导和VEGF减少。  |  Bardag-Gorce, F., et al. 2023. Heliyon. 9: e13397. PMID: 36846655
  8. 血栓素原1和Reelin通过Vldlr调节心脏生长和修复。  |  Pei, L., et al. 2024. Basic Res Cardiol. 119: 169-192. PMID: 38147128
  9. 血栓软蛋白-4是一种在发育中和成年神经系统中表达的细胞外基质蛋白,可促进神经元的生长。  |  Arber, S. and Caroni, P. 1995. J Cell Biol. 131: 1083-94. PMID: 7490284
  10. 血栓软蛋白酶 3 是一种五聚分子,通过涉及两个半胱氨酸残基的链间二硫键连接在一起。  |  Qabar, A., et al. 1995. J Biol Chem. 270: 12725-9. PMID: 7759526
  11. 凝血酶原的功能及其在生理和病理生理学中的参与。  |  Lahav, J. 1993. Biochim Biophys Acta. 1182: 1-14. PMID: 8347680
  12. 血栓软蛋白-1。  |  Adams, JC. 1997. Int J Biochem Cell Biol. 29: 861-5. PMID: 9304800

订购信息

产品名称产品编号规格价格数量收藏夹

Thrombospondin 1 抗体 (A6.1)

sc-59887
200 µg/ml
$316.00

Thrombospondin 1 (A6.1): m-IgG Fc BP-HRP 套装

sc-526945
200 µg Ab; 10 µg BP
$354.00

Thrombospondin 1 (A6.1): m-IgG1 BP-HRP 套装

sc-532318
200 µg Ab; 20 µg BP
$354.00

Thrombospondin 1 抗体 (A6.1) Alexa Fluor® 546

sc-59887 AF546
200 µg/ml
$357.00

Thrombospondin 1 抗体 (A6.1) Alexa Fluor® 594

sc-59887 AF594
200 µg/ml
$357.00

Thrombospondin 1 抗体 (A6.1) Alexa Fluor® 680

sc-59887 AF680
200 µg/ml
$357.00

Thrombospondin 1 抗体 (A6.1) Alexa Fluor® 790

sc-59887 AF790
200 µg/ml
$357.00

What is the recommended dilution for OCT IF?

Asked by: Marina
Thank you for your question. Thrombospondin 1 (A6.1) is recommended for detection of reduced and non-reduced Thrombospondin 1 of mouse, rat, human, equine, bovine, porcine and canine origin by immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500)
Answered by: Tech Support Europe
Date published: 2024-11-14

Where do antibodies bind in TSP-1?

Asked by: doyodo
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-12-24

Can this antibody detect Thrombospondin 1 in non-reduced conditions?

Asked by: AbPolly
Thank you for your inquiry. Yes, this antibody is recommended for detection of both reduced and non-reduced Thrombospondin 1.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by from Tsp1 antibodyTsp1 antibody used for checking expression level in TM cells used western analysis.
Date published: 2023-06-30
Rated 4 out of 5 by from 良い抗体です。培養細胞、培養上清、血清ウェスタンブロッティングに使用しています。よく染まります。つぎは蛍光染色にも使ってみます。
Date published: 2023-03-14
Rated 5 out of 5 by from Strong and specific bindingWe could easily detect TSP at around 250 kD with 1/500 dilution
Date published: 2018-04-24
Rated 4 out of 5 by from Good and clear results by WBI have got specific bands at 1:500 dilution, low background.
Date published: 2017-09-27
Rated 5 out of 5 by from trustworthyThis antibody is very good, we got specific staining at last.
Date published: 2017-05-10
Rated 5 out of 5 by from Very good IHCStrong and specific staining observed by IHC in paraffin embedded sections
Date published: 2017-04-10
Rated 5 out of 5 by from Excellent antibodyVery pleased with western blot data; strong signal with little or no backgound
Date published: 2017-01-03
Rated 5 out of 5 by from Reliable antibody with broad reactivity.A great choice for detecting Thrombospondin 1 in a variety of samples. WB results are clear and specific. IHC staining will provide great results. It has been cited several times.
Date published: 2016-12-23
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Thrombospondin 1 抗体 (A6.1) is rated 4.9 out of 5 by 15.
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