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Thrombin R 抗体 (C-11): sc-271100

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说明书
  • Thrombin R抗体(C-11)是小鼠单克隆IgG2a (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于human物种的Thrombin R的N-terminus附近的氨基酸37-63之间
  • 推荐用于 mouse, rat 和 human 来源的Thrombin R WB, IP, IF 和 ELISA检测
  • 看 Thrombin R (ATAP2): sc-13503,可查看有标记的 Thrombin R 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是Thrombin R Antibody (C-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Thrombin R Antibody (C-11) 打包提供(请参阅下面的订购信息)。
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    関連項目

    凝血酶是一种丝氨酸蛋白酶,参与血小板聚集和血液凝固。它从其前体凝血酶原中裂解出来,并在凝血级联的最后一步中将纤维蛋白原转化为纤维蛋白。凝血酶通过激活细胞表面受体来调节其效应。这些受体,包括凝血酶R(也称为PAR-1,即蛋白酶激活受体-1)、PAR-2和PAR-3,是G蛋白偶联受体家族的成员,并共享相似的基因结构。凝血酶裂解其受体,释放一种作为血小板激动剂的41个氨基酸肽。在凝血酶的这种激活下,凝血酶Rs触发胞质Ca(2+)浓度的增加。未活化的凝血酶R在细胞表面和细胞内池之间循环,而活化的凝血酶R迅速内化并在溶酶体中降解。人凝血酶R还已知受Sp1和Sp3转录因子的调节。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Thrombin R 抗体 (C-11) 参考文献:

    1. 星中的PAR:蛋白酶激活受体和星状细胞功能。关注"凝血酶(PAR-1)通过MAPK诱导星状细胞增殖涉及多种信号通路"。  |  Hollenberg, MD. 2002. Am J Physiol Cell Physiol. 283: C1347-50. PMID: 12372795
    2. 暴露于凝血酶的人肺成纤维细胞中依赖 PAR-1 和不依赖 PAR-1 的促炎信号传导。  |  Ortiz-Stern, A., et al. 2012. J Cell Physiol. 227: 3575-84. PMID: 22278285
    3. 凝血酶/PAR-1 在 ADP 诱导的血小板分泌和第二波聚集中发挥关键作用。  |  Jiang, L., et al. 2013. J Thromb Haemost. 11: 930-40. PMID: 23406164
    4. 凝血酶通过 PAR-1, PKC 和 ERK1/2 通路诱导 A549 细胞的上皮-间质转化。  |  Song, JS., et al. 2013. Exp Lung Res. 39: 336-48. PMID: 23919450
    5. 标记/Par-1标记迁移神经元的极性。  |  Reiner, O. and Sapir, T. 2014. Adv Exp Med Biol. 800: 97-111. PMID: 24243102
    6. 刺激PAR-1或PAR-4可促进类似VEGF和内皮抑素释放的模式,以及由人血小板介导的促血管生成反应。  |  Etulain, J., et al. 2015. Platelets. 26: 799-804. PMID: 26082997
    7. 外在途径诱导的凝血酶和 PAR-1 调节骨折修复部位的炎症。  |  Sato, N., et al. 2016. Bone. 83: 23-34. PMID: 26475502
    8. 凝血酶激活PAR-1导致镰状细胞病小鼠模型中的微血管淤滞。  |  Sparkenbaugh, EM., et al. 2020. Blood. 135: 1783-1787. PMID: 31977004
    9. 丹参酮 IIA 通过 PAR-1 和 p38 MAPK 信号通路抑制凝血酶诱导的人脐静脉内皮细胞组织因子的表达  |  Zhang, Z., et al. 2022. Acta Haematol. 145: 517-528. PMID: 35598597
    10. 蛋白酶激活受体-1(PAR-1)在肾小球滤过屏障完整性中的作用。  |  Medipally, A., et al. 2022. Physiol Rep. 10: e15343. PMID: 35923090

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Thrombin R 抗体 (C-11)

    sc-271100
    200 µg/ml
    $322.00

    Thrombin R (C-11): m-IgG Fc BP-HRP 套装

    sc-540203
    200 µg Ab; 10 µg BP
    $361.00

    Thrombin R (C-11): m-IgG2a BP-HRP 套装

    sc-546873
    200 µg Ab; 10 µg BP
    $361.00

    Thrombin R (C-11) 中和勝肽

    sc-271100 P
    100 µg/0.5 ml
    $69.00

    Can it(sc-271100) be applied to IHC or ICC?

    Asked by: Hugo Bokelu
    This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
    Answered by: Technical Support
    Date published: 2019-05-25

    What application is the blocking peptide sc-271100 P appropriate for?

    Asked by: AbPolly
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by ET from GoodGood, clear band in WB in KNRK whole cell lysate. -SCBT QC
    Date published: 2015-03-07
    Rated 5 out of 5 by MS from Strong band in WB for Thrombin R recombinantStrong band in WB for Thrombin R recombinant fusion protein. -SCBT QC
    Date published: 2014-10-24
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    Thrombin R 抗体 (C-11) is rated 5.0 out of 5 by 2.
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