TCP-1 θ Antibody (E-7): sc-377261
- TCP-1 θ Antibody (E-7) is a mouse monoclonal IgG2b κ TCP-1 θ antibody, cited in 8 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of TCP-1 θ of human origin
- TCP-1 theta Antibody (E-7) is recommended for detection of TCP-1 θ of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-TCP-1 theta Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for TCP-1 θ Antibody (E-7) for WB applications. This reagent is now offered in a bundle with TCP-1 θ Antibody (E-7) (see ordering information below).
TCP-1 θ Antibody (E-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects TCP-1 theta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TCP-1 θ (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCP-1, also known as t complex polypeptide 1, is a crucial subunit of the chaperonin containing TCP-1 (CCT) complex, which is essential for proper folding of cytosolic proteins, particularly actins and tubulins. CCT complex is composed of eight distinct subunits, each encoded by different genes, and plays a vital role in cellular processes by ensuring proteins achieve functional conformations. TCP-1′s importance is underscored by involvement in developmental processes, as TCP-1 was first identified in mice with tail-less and embryonic lethal phenotypes, indicating a critical role in early development. Additionally, homologous sequences of TCP-1 have been found in various species, with yeast TCP-1 serving as a molecular chaperone for actin and tubulin, further highlighting evolutionary conservation and significance in maintaining cellular integrity and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TCP-1 θ Antibody (E-7) References:
- Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins. | Yokota, SI., et al. 2000. Eur J Biochem. 267: 1658-64. PMID: 10712596
- Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress. | Campos, EG. and Hamdan, FF. 2000. Parasitol Res. 86: 253-8. PMID: 10726997
- Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. | Hynes, GM. and Willison, KR. 2000. J Biol Chem. 275: 18985-94. PMID: 10748209
- Defining the eukaryotic cytosolic chaperonin-binding sites in human tubulins. | Ritco-Vonsovici, M. and Willison, KR. 2000. J Mol Biol. 304: 81-98. PMID: 11071812
- Cucumber T-complex protein. Molecular cloning, bacterial expression and characterization within a 22-S cytosolic complex in cotyledons and hypocotyls. | Ahnert, V., et al. 1996. Eur J Biochem. 235: 114-9. PMID: 8631317
- A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development. | Iijima, M., et al. 1998. Gene. 213: 101-6. PMID: 9630545
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCP-1 θ Antibody (E-7) | sc-377261 | 200 µg/ml | $322.00 | |||
TCP-1 θ Antibody (E-7): m-IgG2b BP-HRP Bundle | sc-548764 | 200 µg Ab; 10 µg BP | $361.00 | |||
TCP-1 θ Antibody (E-7) AC | sc-377261 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TCP-1 θ Antibody (E-7) HRP | sc-377261 HRP | 200 µg/ml | $322.00 | |||
TCP-1 θ Antibody (E-7) FITC | sc-377261 FITC | 200 µg/ml | $336.00 | |||
TCP-1 θ Antibody (E-7) PE | sc-377261 PE | 200 µg/ml | $349.00 | |||
TCP-1 θ Antibody (E-7) Alexa Fluor® 488 | sc-377261 AF488 | 200 µg/ml | $364.00 | |||
TCP-1 θ Antibody (E-7) Alexa Fluor® 546 | sc-377261 AF546 | 200 µg/ml | $364.00 | |||
TCP-1 θ Antibody (E-7) Alexa Fluor® 594 | sc-377261 AF594 | 200 µg/ml | $364.00 | |||
TCP-1 θ Antibody (E-7) Alexa Fluor® 647 | sc-377261 AF647 | 200 µg/ml | $364.00 | |||
TCP-1 θ Antibody (E-7) Alexa Fluor® 680 | sc-377261 AF680 | 200 µg/ml | $364.00 | |||
TCP-1 θ Antibody (E-7) Alexa Fluor® 790 | sc-377261 AF790 | 200 µg/ml | $364.00 | |||
TCP-1 θ (E-7) Neutralizing Peptide | sc-377261 P | 100 µg/0.5 ml | $69.00 |