Date published: 2025-9-9
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SphK1 Antibody (G-11) - Western Blotting - Image 362206
SphK1 抗体 (G-11): sc-365401. RAW 264. 7 (A), A-10 (B), U-87 MG (C), HT-1080 (D) 和 MCF7 (E) 全细胞裂解液中 SphK1 表达的 Western 印迹分析.
SphK1 抗体 (G-11): sc-365401. RAW 264. 7 (A), A-10 (B), U-87 MG (C), HT-1080 (D) 和 MCF7 (E) 全细胞裂解液中 SphK1 表达的 Western 印迹分析.

SphK1 抗体 (G-11): sc-365401

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说明书
  • SphK1 抗体 G-11 是小鼠单克隆 IgG1 κ，SphK1抗体, 在13篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的SphK1的C-terminus附近的氨基酸331-351之间
  • SphK1 抗体 (G-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 SphK1
  • 抗SphK1抗体（G-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SphK1 (G-11): sc-365401的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是SphK1 Antibody (G-11) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与SphK1 Antibody (G-11) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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研究信息

関連項目

额外SphK抗体，包括SphK2

SphK1抗体（G-11）是一种IgG1 κ小鼠单克隆SphK1抗体（也称为SPHK1抗体、SK1抗体、SPHK抗体、SPK抗体或鞘氨醇激酶1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的SphK1蛋白。SphK1抗体（G-11）既可作为非共轭抗SphK1抗体形式，也可作为多种共轭形式的抗SphK1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。鞘氨醇激酶（SphK或SphK1）是一种关键酶，催化鞘氨醇的磷酸化以形成鞘氨醇1-磷酸（SPP或S1P）。SPP是一种生物活性脂质，在多种细胞类型中发挥多种生物学效应，既可作为细胞内信使，也可作为与Edg家族受体结合的细胞外配体。SphK1的竞争性抑制剂可阻断SPP的形成，并选择性抑制由多种因素诱导的细胞增殖。SphK1活性的一个有效抑制剂是DMS（N,N-二甲基鞘氨醇）。SPP/SphK1已被认为是一种信号通路，可调节多种细胞功能，包括细胞生长、增殖和存活。具体来说，SphK1参与保护人肝细胞免受TNFα凋亡作用的信号通路。此外，SPP/SphK1可能通过调节SAPKs和caspases的激活在神经元存活中发挥重要作用。SphK1在成人肝脏、肾脏、心脏和骨骼肌中的表达水平最高，但SphK1的激活使细胞脱离其肝特异性表型。SphK1与SphK2高度同源，SphK2是一类不断增长的鞘脂激酶中的另一个成员。SphK2 mRNA的表达表现出与SphK1明显不同的组织分布，并出现在胚胎发育的较晚阶段。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SphK1 抗体 (G-11) 参考文献:

  1. 新型哺乳动物鞘氨醇激酶 2 型同工酶的分子克隆和功能表征。  |  Liu, H., et al. 2000. J Biol Chem. 275: 19513-20. PMID: 10751414
  2. 鞘氨醇激酶的致癌作用  |  Xia, P., et al. 2000. Curr Biol. 10: 1527-30. PMID: 11114522
  3. 肾上腺素激酶的表达调控 PC12 细胞的凋亡和 Caspase 激活。  |  Edsall, LC., et al. 2001. J Neurochem. 76: 1573-84. PMID: 11238741
  4. TNF-α 诱导的 1-磷酸鞘磷脂通过磷脂酰肌醇 3- 激酶/Akt 途径抑制人肝细胞的细胞凋亡。  |  Osawa, Y., et al. 2001. J Immunol. 167: 173-80. PMID: 11418646
  5. 鞘氨醇激酶调节肝癌细胞分化：肝细胞核因子和视黄醇受体的作用  |  Osawa, Y., et al. 2001. Biochem Biophys Res Commun. 286: 673-7. PMID: 11520048
  6. 1- 磷酸肾上腺素通过 Edg3 和 Edg5 受体在 C2C12 肌母细胞中唤起钙信号。  |  Meacci, E., et al. 2002. Biochem J. 362: 349-57. PMID: 11853542
  7. 新型鞘氨醇激酶-1 结合蛋白 RPK118 的鉴定和特征描述  |  Hayashi, S., et al. 2002. J Biol Chem. 277: 33319-24. PMID: 12077123
  8. SPHK1 缺乏可保护小鼠免受乙酰氨基酚诱导的ER应激和线粒体通透性转换的影响。  |  Li, L., et al. 2020. Cell Death Differ. 27: 1924-1937. PMID: 31827236
  9. miR-19a/b-3p 通过 SIRT1/FoxO3/SPHK1 通路促进脑缺血再灌注损伤过程中的炎症反应。  |  Zhou, F., et al. 2021. J Neuroinflammation. 18: 122. PMID: 34051800
  10. 二氢杨梅素通过抑制SPHK1/mTOR信号传导和靶向铁凋亡减轻脑缺血再灌注损伤  |  Xie, J., et al. 2022. Drug Des Devel Ther. 16: 3071-3085. PMID: 36118165
  11. 靶向 SphK1/S1P/PFKFB3 轴可通过破坏推动肿瘤血管生成的糖酵解能量供应来抑制肝细胞癌的进展。  |  Liu, XT., et al. 2024. J Transl Med. 22: 43. PMID: 38200582
  12. SPHK1/S1P/S1PR 通路通过间皮细胞-间充质转化促进腹膜纤维化的进展。  |  Zhao, T., et al. 2024. FASEB J. 38: e23417. PMID: 38226856

订购信息

产品名称产品编号规格价格数量收藏夹

SphK1 抗体 (G-11)

sc-365401
200 µg/ml
$316.00

SphK1 (G-11): m-IgGκ BP-HRP 套装

sc-522234
200 µg Ab, 40 µg BP
$354.00

SphK1 抗体 (G-11) AC

sc-365401 AC
500 µg/ml, 25% agarose
$416.00

SphK1 抗体 (G-11) HRP

sc-365401 HRP
200 µg/ml
$316.00

SphK1 抗体 (G-11) FITC

sc-365401 FITC
200 µg/ml
$330.00

SphK1 抗体 (G-11) PE

sc-365401 PE
200 µg/ml
$343.00

SphK1 抗体 (G-11) Alexa Fluor® 488

sc-365401 AF488
200 µg/ml
$357.00

SphK1 抗体 (G-11) Alexa Fluor® 546

sc-365401 AF546
200 µg/ml
$357.00

SphK1 抗体 (G-11) Alexa Fluor® 594

sc-365401 AF594
200 µg/ml
$357.00

SphK1 抗体 (G-11) Alexa Fluor® 647

sc-365401 AF647
200 µg/ml
$357.00

SphK1 抗体 (G-11) Alexa Fluor® 680

sc-365401 AF680
200 µg/ml
$357.00

SphK1 抗体 (G-11) Alexa Fluor® 790

sc-365401 AF790
200 µg/ml
$357.00

SphK1 (G-11) 中和勝肽

sc-365401 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365401 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good antibody for Western blotProduced positive Western blot data of SphK1 expression in mouse mesenteric arteries extracts.
Date published: 2022-02-09
Rated 5 out of 5 by from Works greatTried the SphK1 antibody for looking at expression in mesenteric arteries using western blot. Validated the bands with knockdown using siRNA.
Date published: 2020-07-22
Rated 5 out of 5 by from Great... Ab works excellent...Thank you Santacruz. The sample of SphK1 Ab you have supplied was awsome. Good results. :-)
Date published: 2018-02-12
Rated 4 out of 5 by from Specific for WBTested on SPHK1 K.O. together with control on Brain tissue. Showed no bands in the mutant. However, a bit dirty.
Date published: 2018-01-04
Rated 1 out of 5 by from Does not recognize SphK1While this antibody recognizes a band in the correct MW range of SphK1, it does not appear to actually be specific for SphK1. We tested the antibody by western blotting using wild type and SphK1 KO knockout cell lysate as well as with recombinant crystallography grade SphK1 as a positive control. Both cell lysates were immunopositive, but the positive control was not. If you would like me to upload images please let me know. We did receive this antibody as a free sample, and our lab has extensive experience with this protein.
Date published: 2017-12-04
Rated 4 out of 5 by from Working well SphK1 antibody in WBThis SphK1 antibody works well in WB with human liver and mouse liver, kidney and brain.
Date published: 2017-10-12
Rated 5 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium-strong detection of the ~42 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced nice Western blot data of SphK1Produced nice Western blot data of SphK1 expression in PC-12 whole cell lysate. -SCBT QC
Date published: 2013-06-15
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SphK1 抗体 (G-11) is rated 4.3 out of 5 by 8.
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