Date published: 2025-9-7

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spectrin α II 抗体 (C-11): sc-46696

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说明书
  • spectrin α II抗体(C-11)是小鼠单克隆IgG1 κ, 在31篇文献中引用,规格为200 µg/ml
  • 免疫human物种的spectrin α II的氨基酸2368-2472
  • 抗-spectrin alpha II 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 spectrin α II
  • 抗spectrin alpha II抗体(C-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取spectrin α II (C-11): sc-46696免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 spectrin α II 抗体 (C-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与spectrin α II 抗体 (C-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

spectrin α II 抗体(C-11)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过多种应用检测小鼠、大鼠和人类样本中的spectrin α II,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)。anti -spectrin α II 抗体(C-11)有非结合型和多种结合型,如琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和各种 Alexa Fluor® 结合物。spectrin α II, 也称为fodrin α,在维持非红细胞细胞骨架的结构完整性方面发挥着至关重要的作用,spectrin α II主要位于细胞质中。spectrin α II对于细胞膜的组织和稳定至关重要,因为spectrin α II形成了一个网络,支持细胞形状和机械特性。spectrin细胞骨架网络对于细胞信号传导、运动和维持细胞整体结构等各种细胞过程至关重要。此外,spectrin α II与其他细胞骨架蛋白(如肌动蛋白和各种膜蛋白)相互作用,进一步强调了spectrin α II在细胞动力学和完整性中的重要性。钙蛋白酶激活导致spectrin α II分解,可导致神经元结构发生重大变化,从而凸显spectrin α II在神经退行性疾病过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

spectrin α II 抗体 (C-11) 参考文献:

  1. Spectrin alpha II和beta II异构体在四聚体化位点具有高亲和力相互作用。  |  Bignone, PA. and Baines, AJ. 2003. Biochem J. 374: 613-24. PMID: 12820899
  2. 大鼠脑局部缺血后,二氢嘧啶酶相关蛋白2、αII链、热休克同源蛋白70假基因1和肌动蛋白调节蛋白2的表达上调--一种蛋白质组学方法。  |  Chen, A., et al. 2007. Neurochem Int. 50: 1078-86. PMID: 17196711
  3. TRH 及其类似物对各种兴奋毒性, 坏死和凋亡因子诱导的原发性皮质神经细胞损伤的影响。  |  Jantas, D., et al. 2009. Neuropeptides. 43: 371-85. PMID: 19666192
  4. 在大鼠发育过程中,alpha II-spectrin心脏选择性可变剪接变体cardi+的表征和表达。  |  Zhang, Y., et al. 2010. J Mol Cell Cardiol. 48: 1050-9. PMID: 20114050
  5. 血清SNTF是轴突损伤的替代标记物,可预测急诊科治疗轻度脑创伤后持续性脑功能障碍。  |  Siman, R., et al. 2020. Front Neurol. 11: 249. PMID: 32322237
  6. Spna2 R1098Q小鼠变体的进行性共济失调、记忆障碍和癫痫发作影响αII 斯普林蛋白的支架稳定性。  |  Zalas, M., et al. 2023. Brain Sci. 13: PMID: 36831804
  7. Erk 抑制是治疗高 IL-8 分泌和低 SPTAN1 表达结直肠癌的有效策略。  |  Meier, C., et al. 2024. Int J Mol Sci. 25: PMID: 38891846
  8. 遗传性椭圆细胞症中的异常spectrin。  |  Marchesi, SL., et al. 1986. Blood. 67: 141-51. PMID: 3940543
  9. 两个患有遗传性椭圆细胞性贫血的黑人家庭中,斯佩克林分子的病理和非病理变异。  |  Lecomte, MC., et al. 1985. Hum Genet. 71: 351-7. PMID: 4077050
  10. 红细胞β-spectrin(β I sigma 2)的270 kDa剪接变异体在体内和体外与小脑神经元的特定区域分离。  |  Malchiodi-Albedi, F., et al. 1993. J Cell Sci. 106 (Pt 1): 67-78. PMID: 8270644

订购信息

产品名称产品编号规格价格数量收藏夹

spectrin α II 抗体 (C-11)

sc-46696
200 µg/ml
$316.00

spectrin α II (C-11): m-IgG Fc BP-HRP 套装

sc-526727
200 µg Ab; 10 µg BP
$354.00

spectrin α II (C-11): m-IgG1 BP-HRP 套装

sc-532100
200 µg Ab; 20 µg BP
$354.00

spectrin α II 抗体 (C-11) AC

sc-46696 AC
500 µg/ml, 25% agarose
$416.00

spectrin α II 抗体 (C-11) HRP

sc-46696 HRP
200 µg/ml
$316.00

spectrin α II 抗体 (C-11) FITC

sc-46696 FITC
200 µg/ml
$330.00

spectrin α II 抗体 (C-11) PE

sc-46696 PE
200 µg/ml
$343.00

spectrin α II 抗体 (C-11) Alexa Fluor® 488

sc-46696 AF488
200 µg/ml
$357.00

spectrin α II 抗体 (C-11) Alexa Fluor® 546

sc-46696 AF546
200 µg/ml
$357.00

spectrin α II 抗体 (C-11) Alexa Fluor® 594

sc-46696 AF594
200 µg/ml
$357.00

spectrin α II 抗体 (C-11) Alexa Fluor® 647

sc-46696 AF647
200 µg/ml
$357.00

spectrin α II 抗体 (C-11) Alexa Fluor® 680

sc-46696 AF680
200 µg/ml
$357.00

spectrin α II 抗体 (C-11) Alexa Fluor® 790

sc-46696 AF790
200 µg/ml
$357.00

n my mitochondrial proteomic analysis of mice, the protein spectrin appeared very expressed, would you have a suggestion of the best antibody to label by WB, and have any antibodies that mark both subunits?

Asked by: tamara Aeaujo
Thank you for your question. This is a very good antibody for detection of spectrin α II of mouse origin in Western blotting. Please contact our Technical Service Department for further recommendations.
Answered by: Technical Support
Date published: 2019-02-10

What is the recommended secondary reagent to use with spectrin α II (C-11): sc-46696 mouse monoclonal primary?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with spectrin α II (C-11): sc-46696, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-10
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Rated 5 out of 5 by from Works well in WBSingle band observed in western blot using HeLa whole cell extract (40 ug).
Date published: 2019-11-06
Rated 5 out of 5 by from Works wellWorks well with human cancer cell lines and non-cancer cells in WB and IF (methanol and PFA fixation method) detecting endogenous spectrin alpha II .
Date published: 2017-05-28
Rated 5 out of 5 by from Great antibodyGreat antibody for western blot, We got a very good result atdilution 1:800.
Date published: 2017-05-13
Rated 5 out of 5 by from Really nice data !!All applications including WB, IF and IHC are outstanding; much better than polyclonal alternatives
Date published: 2017-01-12
Rated 5 out of 5 by from Works great for array of applicationsThis antibody shows strong signal in array of applications, including Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry with paraffin-embedded sections.
Date published: 2016-12-16
Rated 5 out of 5 by from Used in IF in mouse samples and got correctUsed in IF in mouse samples and got correct staining pattern.
Date published: 2015-07-29
Rated 5 out of 5 by from Published in WB in rat astrocytesPublished in WB in rat astrocytes. -SCBT Publication Review
Date published: 2015-05-30
Rated 5 out of 5 by from Good specificity in WB in Hs68 and TFGood specificity in WB in Hs68 and TF-1 whole cell lysates and mouse uterus, rat brain tissue extracts. -SCBT QC
Date published: 2015-04-28
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spectrin α II 抗体 (C-11) is rated 5.0 out of 5 by 11.
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