Date published: 2025-9-6
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Sp1 Antibody (1C6) - Western Blotting - Image 855
Sp1 抗体 (1C6): sc-420. K-562 (A, B) 和 Jurkat (C) 核提取物 (A, C) 及全细胞裂解液 (B) 中 Sp1 表达的 Western 印迹分析.
Sp1 抗体 (1C6): sc-420. K-562 (A, B) 和 Jurkat (C) 核提取物 (A, C) 及全细胞裂解液 (B) 中 Sp1 表达的 Western 印迹分析.

Sp1 抗体 (1C6): sc-420

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说明书
  • Sp1 抗体 1C6 是小鼠单克隆 IgG1 κ，Sp1抗体, 在358篇文献中引用，规格为200 µg/ml
  • 针对氨基酸609-627的抗性，映射到Sp1的内部域，human起源
  • Sp1 抗体 (1C6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Sp1 p95 and p106; 不建议用于IP
  • 抗Sp1抗体（1C6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-420 X, 200 µg/0.1 ml)
  • 关于如何获取Sp1 (1C6): sc-420的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Sp1 抗体 (1C6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Sp1 抗体 (1C6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Sp1抗体（1C6）是一种IgG1 κ小鼠单克隆Sp1抗体（也称为Sp1转录因子抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的Sp1蛋白。Sp1抗体（1C6）既可以是非偶联的抗Sp1抗体形式，也可以是多种偶联形式的抗Sp1抗体形式，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。Sp1是一种序列特异性转录因子，可识别GGGGCGGGGC及其密切相关的序列，这些序列通常被称为GC盒。Sp1最初被确定为来自HeLa细胞的因子，可从SV40启动子选择性地激活体外转录，并结合SV40中21bp重复元件中的多个GC盒。DNA结合的序列特异性由Zn（II）指示器提供，而Sp1的另一个不同区域似乎调节DNA结合的亲和力。证据表明，早期生长反应基因Erg-1（也称为Zif268或NGF1-A）可通过与Sp1竞争结合到重叠结合基序上，来降低某些哺乳动物基因启动子的活性。人类Sp1基因定位于染色体12q13.1。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sp1 抗体 (1C6) 参考文献:

  1. 小鼠腺苷脱氨酶基因启动子中 Sp1 和 Zif268 的重叠共识结合基序的功能意义  |  Ackerman, SL., et al. 1991. Proc Natl Acad Sci U S A. 88: 7523-7. PMID: 1881892
  2. GC 盒的结合诱导 DNA 依赖性蛋白激酶对 Sp1 进行磷酸化。  |  Jackson, SP., et al. 1990. Cell. 63: 155-65. PMID: 2170018
  3. Sp1 的不同区域调节 DNA 结合和转录激活。  |  Kadonaga, JT., et al. 1988. Science. 242: 1566-70. PMID: 3059495
  4. 转录因子 Sp1 的 cDNA 分离及 DNA 结合域的功能分析。  |  Kadonaga, JT., et al. 1987. Cell. 51: 1079-90. PMID: 3319186
  5. 分离可区分 RNA 聚合酶 II 识别的不同启动子的转录因子。  |  Dynan, WS. and Tjian, R. 1983. Cell. 32: 669-80. PMID: 6187469
  6. 启动子特异性转录因子 Sp1 与 SV40 早期启动子的上游序列结合。  |  Dynan, WS. and Tjian, R. 1983. Cell. 35: 79-87. PMID: 6313230
  7. 通过荧光原位杂交将人类 Sp1 转录因子基因定位在 12q13 上。  |  Matera, AG. and Ward, DC. 1993. Genomics. 17: 793-4. PMID: 8244404

订购信息

产品名称产品编号规格价格数量收藏夹

Sp1 抗体 (1C6)

sc-420
200 µg/ml
$316.00

Sp1 (1C6): m-IgG Fc BP-HRP 套装

sc-526457
200 µg Ab; 10 µg BP
$354.00

Sp1 (1C6): m-IgG1 BP-HRP 套装

sc-531830
200 µg Ab; 20 µg BP
$354.00

Sp1 抗体 (1C6) X

sc-420 X
200 µg/0.1 ml
$316.00

Sp1 抗体 (1C6) AC

sc-420 AC
500 µg/ml, 25% agarose
$416.00

Sp1 抗体 (1C6) HRP

sc-420 HRP
200 µg/ml
$316.00

Sp1 抗体 (1C6) FITC

sc-420 FITC
200 µg/ml
$330.00

Sp1 抗体 (1C6) PE

sc-420 PE
200 µg/ml
$343.00

Sp1 抗体 (1C6) Alexa Fluor® 488

sc-420 AF488
200 µg/ml
$357.00

Sp1 抗体 (1C6) Alexa Fluor® 546

sc-420 AF546
200 µg/ml
$357.00

Sp1 抗体 (1C6) Alexa Fluor® 594

sc-420 AF594
200 µg/ml
$357.00

Sp1 抗体 (1C6) Alexa Fluor® 647

sc-420 AF647
200 µg/ml
$357.00

Sp1 抗体 (1C6) Alexa Fluor® 680

sc-420 AF680
200 µg/ml
$357.00

Sp1 抗体 (1C6) Alexa Fluor® 790

sc-420 AF790
200 µg/ml
$357.00

Sp1 (1C6) 中和勝肽

sc-420 P
100 µg/0.5 ml
$68.00

I want to use Sp1 ab for CHIP. and is there any antibody suitable for CHIP analysis ? i can't find the proper product and catalog. thank you

Asked by: dalpu2002
Thank you for your question. We recommend using our mouse monoclonal, Sp1 Antibody (1C6): sc-420 for ChIP. We recommend purchasing the more concentrated TransCruz reagent for ChIP applications (sc-420 X, 200 µg/0.1 ml). This antibody has been published for use in ChIP applications (e.g., PMID's: 16901903, 17059557 and 20351094).
Answered by: Technical Support
Date published: 2017-01-31

Can this mouse monoclonal antibody be used for IHC staining of mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody for Sp1 is available conjugated to agarose (sc-420 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-420 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-420 PE), fluorescein (sc-420 FITC), Alexa Fluor® 488 (sc-420 AF488) or Alexa Fluor® 647 (sc-420 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Answered by: Technical Support 13
Date published: 2017-01-04

What is the difference between sc-420 and sc-420 P?

Asked by: AbPolly
Thank you for your inquiry. sc-420 is our standard unconjugated Sp1 (1C6) monoclonal antibody. sc-420 P is the blocking peptide matching this antibody that can be used as a negative control in combination with the antibody. For the protocol on how to use the blocking peptide, please see here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Service
Date published: 2016-12-23
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Rated 5 out of 5 by from SP1(1C6)抗体很nice2个月前申请了试用装，做coip，成功拉出我的目的分子。不知道啥原因，用CST的SP1抗体反而拉不出来我的目的分子。
Date published: 2024-09-24
Rated 5 out of 5 by from Sp1 AbGood antibody for detecting Sp1 in mouse tissue (IHC, 1:100).
Date published: 2024-02-12
Rated 1 out of 5 by from Did not work for meThis did not work for me on Western blot with 50 ug of HeLa lysate at 1:250 dilution. I saw some weak non-specific bands and nothing on the size range of Sp1. It might work for other applications or with other tissues/cells. This is all I tested it with.
Date published: 2019-07-04
Rated 1 out of 5 by from Not workingAntibody for IF cryostat sections mouse retina does not work
Date published: 2018-03-27
Rated 5 out of 5 by from Nice qualityI ever used abcam antibody but very weak band .When I use this antibody detect human Gastric cancer by dilution 1:200,we successfully got strong band .
Date published: 2018-01-27
Rated 4 out of 5 by from nice bandtested on rat tissue samples in western blot and gave a nice band.
Date published: 2017-12-13
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were from human cells in culture
Date published: 2017-11-21
Rated 2 out of 5 by from Not particularly good, some nonspecific bandsa free sample！ Although some nonspecific bands are present, i hope to be able to perform well in the IP experiment
Date published: 2017-08-15
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Sp1 抗体 (1C6) is rated 4.0 out of 5 by 21.
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